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Liu H.,Southwest University | Liu H.,China Institute of Technology | Du R.,Liangshan Branch of Sichuan Provincial Tobacco Company | Zhao J.,Zunyi Branch of Guizhou Provincial Tobacco Company | And 3 more authors.
Tobacco Science and Technology | Year: 2016

To investigate the conversion and supply mechanism of soil nutrients in tobacco field, determine scientific fertilization procedure and construct healthy soil environment, a field experiment was conducted with three soil treatments: without fertilizers (CK), with chemical fertilizer (CF), with organic manure and chemical fertilizer combination (MCF). Soil enzyme activities and bacterial community structures in the rhizosphere of flue-cured tobacco were studied. The results showed that comparing with CK, there was an increment of microbial carbon and nitrogen and a stimulation of enzymatic activities including dehydrogenase, invertase and urease in the soil of MCF treatment. The numbers of OTUs of 16S rDNA in the soils of MCF, CF and CK treatments were 291, 245 and 208, respectively; which belonged to 17 bacterial phyla, such as Proteobacteria, Actinobacteria, Firmicutes, etc. There were a lot of Nitrobacter and Nitrosospira. Among 20 dominant bacteria, the abundance of Cellulomonas, Fibrobacter, Coprococcus and Enterococcus was higher in the soil of MCF treatment than that of CK treatment, which was beneficial to organic matter mineralization and organic fertilizer utilization. Comparing with CK treatment, the abundance of Desulfobacter and Nocrdioides increased, and that of Acinetobacter decreased, while Rhizobium disappeared in the rhizosphere soil of CF treatment. The uniformity index and diversity index of bacterial community in the soil of MCF treatment was significantly promoted, the dominance index significantly reduced. In conclusion, MCF treatment improved soil environment and increased bacterial biodiversity, which were helpful to inhibiting the over reproduction of pathogenic bacteria and decreasing the disease risk of tobacco plants. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Pu D.,Sichuan Academy of Agricultural science | Wang Y.,Liangshan Branch of Sichuan Provincial Tobacco Company | Liu H.,Sichuan Academy of Agricultural science | Ji P.,Sichuan Academy of Agricultural science | And 8 more authors.
Tobacco Science and Technology | Year: 2016

In order to clarify the resource and dominant categories of natural enemies of aphid in different tobacco planting areas of Sichuan, the natural enemies of aphid were investigated, collected and identified from 2014 to 2015 crop years. The results showed that there were 29 species of natural enemies for Myzus persicae (Sulzer) in total, included 10 families of 6 orders. The dominant species were Aphidius gifuensis Ashmaed, Coccinella septempunctata Linnaeus, Harmonia axyridis (Pallas), Chrysopa pallens (Rambur) and Episyrphus balteatus(De Geer). Aphidius gifuensis Ashmaed was the dominant enemy in all areas. In addition, Coccinella septempunctata Linnaeus, Harmonia axyridis (Pallas), Chrysopa pallens (Rambur) and Episyrphus balteatus (De Geer)were the dominant enemies in Liangshan, Panzhihua, Dazhou and Guangyuan, as did Harmonia axyridis (Pallas)in Luzhou, Yibin and Deyang. © 2016, Editorial Office of Tobacco Science & Technology. All right reserved.


Zhang L.,Tobacco Research Institute of CAAS | Liu H.,Tobacco Research Institute of CAAS | Gu J.,Chinese Academy of Agricultural Sciences | Lei Q.,Sichuan Tobacco Monopoly Administration | And 4 more authors.
Tobacco Science and Technology | Year: 2013

In order to investigate the co-effects of compound microbial inoculum and organic-inorganic fertilizer on the resistance, growth, yield and quality of flue-cured tobacco, the activity of various defensive enzymes in tobacco roots and leaves at different growing stages as well as the changes of tobacco yield and quality were determined with field trials. The results indicated that proportionate application of compound microbial inoculum with organic and inorganic fertilizers significantly improved the agronomic character of tobacco, enhanced the activities of defensive enzymes, such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and phenylalanine ammonia lyase (PAL) in tobacco roots and leaves, and reduced the morbidity and disease index relative to tobacco black shank disease, tobacco mosaic virus disease and tobacco brown spot disease. Correlation analysis showed that the activities of defensive enzymes (PAL, SOD, and POD) in tobacco roots positively correlated to those in tobacco leaves. Proportionate application increased the contents of total sugar and reducing sugar in tobacco leaves, especially increased potassium content significantly, which made the chemical composition in tobacco leaves more harmonious. The experiments indicated that the application of compound microbial inoculum together with 75% organic and inorganic fertilizers was beneficial to the growth, development of flue-cured tobacco and increasing its yield, quality and resistance to stress.


Zhan H.,Tobacco Research Institute of CAAS | Shi J.,Tobacco Research Institute of CAAS | Meng K.,Tobacco Research Institute of CAAS | Luo Z.,Tobacco Research Institute of CAAS | And 6 more authors.
Tobacco Science and Technology | Year: 2015

For effectively controlling weeds in tobacco fields, the seed pools of weeds in main tobacco growing areas in Sichuan was studied in terms of composition and population with germination method in 2011-2014. The results showed that there included 24 weed species, belonged to 15 families, spread mainly in 0-15 cm soil layer. The dominant species were of Gramineae, followed by those of Compositae and then of Chenopodiaceae. The species of dicotyledonous weeds exceeded the species of monocotyledonous weeds in number, but the density of seed was obviously lower. The highest density (19119 seeds/m2) seed pool was found in the sample from Guangyuan, while the lowest (6177 seeds/m2) was found in the sample from Panzhihua. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Yao X.,Liangshan Branch of Sichuan Provincial Tobacco Company | Guo Q.,Liangshan Branch of Sichuan Provincial Tobacco Company | Niu X.,Liangshan Branch of Sichuan Provincial Tobacco Company | Zhang X.,Liangshan Branch of Sichuan Provincial Tobacco Company
Tobacco Science and Technology | Year: 2015

In order to shorten the period of seedling culturing and improve the quality of tobacco seedlings, a contrast experiment was conducted by applying seedling fertilizers at different rates into seedling culturing substrates. The results showed that comparing with the control (CK), the period of seedling culturing reduced by 8 days when seedling fertilizers were applied into the substrates at a rate of 520 g/m3. All of seedling growing stages were shortened, particularly the duration from seedling germination to little cross stage decreased by 3.38 days. The number and weight of roots, the agriculture traits, root vitality and the chlorophyll content and nitrate reductase activity of leaves reached to their highest. Therefore, applying appropriate amount (520 g/m3) seedling fertilizers into substrates can promote the growth and development of tobacco seedlings, obviously shorten seedling growing period, and the cultured seedlings possess vigorous and well developed root systems. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

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