LEXVA Analytique

France

LEXVA Analytique

France
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Silou T.,Marien Ngouabi University | Bikanga R.,Université des Sciences et Techniques de Masuku | Nsikabaka S.,Marien Ngouabi University | Nombault J.,Marien Ngouabi University | And 3 more authors.
Biotechnology, Agronomy and Society and Environment | Year: 2017

Description. The essential oils of Cymbopogon nardus (L.) Rendle, Cymbopogon winterianus Jowitt, Cymbopogon flexuosus (Nees ex Steud.) Wats and Cymbopogon citratus L. (DC.) Stapf (lemongrass) consist of more than 80% of the following constituents: Citronellal, geraniol, citral, citronellol, geranyl acetate, and limonene. For the purposes of business transactions, it is therefore important to know the exact chemical composition of the essential oils produced. Objectives. Chemotype evaluation of C. nardus acclimatized on the Plateau des Cataractes. Method. The essential oils of C. nardus were extracted by hydrodistillation and analyzed by chromatography (GC and GC/ MS) over a period of more than ten years. Results. Cymbopogon nardus was found to produce a Java type citronella essential oil (C. winterianus) with the following profile: Citronellal (40-48%), geraniol (10-22%), citronellol (10-12%), limonene (2-3%), geranyl acetate (1-2%), linalool (1%). The difference between these two types of citronella, Java and Ceylon, was based on the relative proportions of their three main constituents: Citronellal, geraniol and citronellol. Cymbopogon nardus is known for its high variability, with two varieties and seven subvarieties. Conclusions. The citronella established in West and Central Africa under the name of Cymbopogon nardus (Java type) could be considered a Cymbopogon winterianus Jowitt, based on the chemical composition of its essential oil. © 2017, FAC UNIV SCIENCES AGRONOMIQUES GEMBLOUX. All rights reserved.


Bouchaala M.,Ferhat Abbas University Setif | Ramdani M.,Ferhat Abbas University Setif | Chalard P.,Institute of Chemistry of Clermont-Ferrand | Chalard P.,CNRS Institute of Chemistry | And 2 more authors.
International Journal of Pharmacognosy and Phytochemical Research | Year: 2016

The hydro-distillation of the essential oil of Helichrysum italicum variety numidicum Pomel gave a viscous liquid with pale yellow oil. The average yield of essential oil of the samples is 0.03%. The chemical composition of essential oil was analyzed by GC and GC/MS. A total 43 compounds representing 92.9% of the oil were identified in H. italicum. The chemical composition is dominated by the presence of major products, Isopropyl tetradecanote (12.10%), α-pinene (12.02%), Hexadecanoic acid (9.96%), Caryophyllene (E) (9.22%), Ledol (9.11%), Palustrol (5.55%), α-Humulene (4.28%), Caryophyllene <9-epi> (3.75%), α-copaene (3.72%), cis-Calamenene (2.18%). The Essential oil of H. italicum was tested for six microorganisms. The oil showed modest effect against all the bacteria tested and it has no significant antifungal activity against the Candida albicans. The population of helichrysum italicum shows a tertraploïde chromosome number of 2n = 28 with basic number x = 7. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.


Kenoufi M.,Ferhat Abbas University Setif | Lograda T.,Ferhat Abbas University Setif | Chalard P.,Institute of Chemistry of Clermont-Ferrand | Chalard P.,CNRS Institute of Chemistry | And 2 more authors.
International Journal of Pharmacognosy and Phytochemical Research | Year: 2016

The species Senecio giganteus is endemic to North Africa, especially in streams of mound. The essential oil isolated from aerial parts of S. giganteus from Ain Roua (Setif) region was submitted to the hydrodistillation; the yield obtained is very low 0.02% (v/w). The oil was analysed by GC and GC/MS. The chemical analysis has allowed identifying 40 compounds corresponding to 92.38 %. The oxygenated sesquiterpenes are dominant in the essential oil of S. giganteus with 21.22% and the hexadecanoic acid is the major component (17.80%), followed by isophytol (12.43%), pentanol 3 methyl (7.28%) and phytol (6.66%). The Essential oil of S. giganteus was tested for antimicrobial activity; it showed a modest effect against the strains tested. The karyological investigation of the population of S. giganteus has revealed a tetraploid chromosome number 2n = 4x = (20 + 2B), this number is reported for the first time in Algeria. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.


Chalchat J.,LEXVA ANALYTIQUE | Ozcan M.,Selcuk University | Figueredo G.,LEXVA ANALYTIQUE | Chalard P.,Laboratoire Of Chimie Des Heterocycles Et Des Glucides
Acta Botanica Hungarica | Year: 2011

The essential oils of leaves of Echinophora tenuifolia subsp. sibthorpiana (Apiaceae) growing wild in Middle Anatolia were obtained by hydrodistillation, and analysed by gas chromatography (GC) and gas chromatography-mass spectrometer (GC-MS). The major constituents were affected depend on harvest years. Methyl-eugenol, δ-3-carene and p-cymene were established as the major components of pickling herb leaves collected between 2003 and 2009. The main constituents of leave oil were methyl-eugenol (24.99% to 90.16%), δ-3-carene (2.57% to 34.80%) and p-cymene (1.23% to 9.81%) depending on the harvest years.


Figueredo G.,Lexva Analytique | Chalchat J.C.,Lexva Analytique | Al Juhaimi F.Y.,King Saud University | Ozcan M.M.,Selcuk University
Asian Journal of Chemistry | Year: 2012

The essential oils of leave of Foeniculum vulgare subsp. piperitum (Apiaceae) growing wild in south Anatolia, Turkey were extracted by hydrodistillation and analyzed by GC and GC-MS. The percentage yields of the essential oils from leaves of bitter fennel harvested in different years (from 2003 to 2008) were 1.3, 3.09, 2.1, 1.95, 1.7 and 1.9 %, respectively. The major constituents varied depending on harvest years. Methyl chavicol, fenchone, limonene and fenchyl acetate (exo) were established as the major components of bitter fennel leaves collected in different years. The main constituents of leave oil were methyl chavicol (24.67-69.93 %), fenchone (7.82-14.50 %), limonene (2.39-9.85 %) and fenchyl acetate (exo) (0.17-14.21 %) among the years.


Lograda T.,Ferhat Abbas University Setif | Ramdani M.,Ferhat Abbas University Setif | Chalard P.,University Blaise Pascal | Figueredo G.,LEXVA Analytique | And 2 more authors.
Journal of Applied Pharmaceutical Science | Year: 2013

The chemical composition of essential oil isolated from Chrysanthemum fontanesii and C. coronarium by hydrodistillation, was analysed by GC and GC/MS. A total 66 compounds representing 97.9% of the oil were identified in C. fontanesii, and 44 components representing 97.2% of the total oil in C. coronarium. The chemical composition of C. fontanesii and C. coronarium, is very different, the only common components are α-pinene, β- pinene, myrcene and δ3-carene. The Essential oil of C. fontanesii and C. coronarium was tested for antibacterial activity against nine bacteria strains. The oil showed modest effect against Staphylococcus aureus ATCC 25923, and it has no significant antibacterial activity against the other bacteria tested. The population of C. fontanesii studied showed a diploid chromosome number 2n = 2x = 18 and a tetraploid 2n = 4x = 36 for C. coronarium. © 2013 Parvin Babaei and Bahram Soltani Tehrani.


Ramdani M.,Ferhat Abbas University Setif | Lograda T.,Ferhat Abbas University Setif | Chalard P.,National Graduate School of Chemistry of Clermont Ferrand | Chalchat J.C.,University Blaise Pascal | Figueredo G.,LEXVA Analytique
Natural Product Communications | Year: 2011

Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Central Sahara of Algeria), were analyzed by gas chomatography coupled to mass spectrometry (GC-MS). Analyses were carried out on 164 trees of 26 natural populations in order to determine the intra-specific variability. Thirty-two terpenoïds were identified, the major ones being α-pinene (11.5-44.2), Δ3-carene (5.7-31.7) and germacrene-D (15.7-54.1). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variability confirmed that genetic factors were not responsible for the decrease in the number of this species, the main reason probably being the Tassili n'Ajjer desertification.


Figueredo G.,Lexva Analytique | Unver A.,Selcuk University | Chalchat J.C.,Lexva Analytique | Arslan D.,Selcuk University | Ozcan M.M.,Selcuk University
Journal of Food Biochemistry | Year: 2012

Chemical compositions of essential oils obtained by hydrodistillation and microwave extraction systems from sage (Salvia fruticosa Mill.), rosemary (Rosmarinus officinalis L.), basil (Ocimum basilicum L.), summer savory (Satureja hortensis L.), winter savory (Satureja thymbra L.) and mint (Mentha spicata L.) were determined with gas chromatography and gas chromatography-mass spectrometry. Carvacrol (38.72 and 34.03%), thymol (17.15 and 14.36%) and p-cymene (12.26 and 18.32%) were determined in high concentrations in the savory oil extracted by both microwave and hydrodistillation extraction systems. The most important ones for rosemary were 1,8-cineole, camphene and borneol. The main components of essential oil of sage obtained by both methods were 52.96 and 51.25% 1,8-cineole, 3.11 and 4.41% alfa-pinene. The major components in essential oil of mint were determined as 80.63 and 68.46% carvone, in decreasing order. The dominant components of essential oil of basil obtained by both microwave distillation or clevenger distillation method were; 30.44 and 34.90% linalool, 25.48 and 31.40% methyl chavicol, and 19.31 and 13.19% (E)-methyl cinnamate. The major components of Satureja hortensis were identified as p-cymene (10.95 and 19.93%), γ-terpinene (8.33 and 20.53%) and carvacrol (59.35 and 38.83%). © 2011 Wiley Periodicals, Inc.


Traore N.,University Of Bamako | Sidibe L.,University Of Bamako | Figueredo G.,LEXVA Analytique | Chalchat J.-C.,AVAHEA | Chalchat J.-C.,University Blaise Pascal
Journal of Essential Oil Research | Year: 2010

The chemical composition of essential oils isolated from leaves of Eucalyptus houseana, E. citriodora, E. raveretiana, E. robusta and E. urophylla collected at N'Débougou (Mali) was determined by gas chromatography (GC) and GC coupled with mass spectrometry (GC/MS). Sixty-seven compounds were identified. The main constituents were α-pinene (1.3-23.9%), p-cymene (0.3-30.6%), 1,8-cineole (12.3-32.1%), γ-terpinene (0.2-22.3%) and citronellal, present only in E. citriodora (76.3%). © 2010 Allured Business Media.


PubMed | LEXVA ANALYTIQUE
Type: Journal Article | Journal: Natural product research | Year: 2012

The essential oils of sage leaves (Salvia aucheri Bentham var. canescens Boiss. & Heldr.), growing wild in South Anatolia, were extracted by hydrodistillation and analysed by GC and GC-MS. The percentage yields of the essential oils from sage leaves harvested at different years were 1.0%, 1.3%, 1.3%, 1.0%, 1.4%, 1.5% and 1.2%, respectively. In this study, 1,8-cineole, camphre, camphene -pinene and -pinene were identified as the major components of sage leaves collected at different periods. The main constituents of sage oil collected over the years were 1,8-cineole (35.01-48.06%), camphre (13.58-23.92%), camphene (6.77-8.82%), -pinene (5.79-8.54%) and -pinene (4.32-6.28%).

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