LEXVA Analytique


LEXVA Analytique

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Bayala B.,University of Ouagadougou | Bayala B.,University Blaise Pascal | Bayala B.,French National Center for Scientific Research | Bayala B.,French Institute of Health and Medical Research | And 15 more authors.
PLoS ONE | Year: 2014

This research highlights the chemical composition, antioxidant, anti-inflammatory and anti-proliferative activities of essential oils from leaves of Ocimum basilicum, Ocimum americanum, Hyptis spicigera, Lippia multiflora, Ageratum conyzoides, Eucalyptus camaldulensis and Zingiber officinale. Essential oils were analyzed by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector. Major constituents were α-terpineol (59.78%) and β-caryophyllene (10.54%) for Ocimum basilicum; 1, 8-cineol (31.22%), camphor (12.730%), α-pinene (6.87%) and trans α-bergamotene (5.32%) for Ocimum americanum; β-caryophyllene (21%), α-pinene (20.11%), sabinene (10.26%), β-pinene (9.22%) and α-phellandrene (7.03%) for Hyptis spicigera; p-cymene (25.27%), β-caryophyllene (12.70%), thymol (11.88), γ-terpinene (9.17%) and thymyle acetate (7.64%) for Lippia multiflora; precocene (82.10%)for Ageratum conyzoides; eucalyptol (59.55%), α-pinene (9.17%) and limonene (8.76%) for Eucalyptus camaldulensis; arcurcumene (16.67%), camphene (12.70%), zingiberene (8.40%), β-bisabolene (7.83%) and β-sesquiphellandrène (5.34%) for Zingiber officinale. Antioxidant activities were examined using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2′-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. O. basilicum and L. multiflora exhibited the highest antioxidant activity in DPPH and ABTS tests, respectively. Anti-inflammatory properties were evaluated by measuring the inhibition of lipoxygenase activity and essential oil of Z. officinale was the most active. Anti-proliferative effect was assayed by the measurement of MTT on LNCaP and PC-3 prostate cancer cell lines, and SF-763 and SF-767 glioblastoma cell lines. Essential oils from A. conyzoides and L. multiflora were the most active on LNCaP and PC-3 cell lines, respectively. The SF-767 glioblastoma cell line was the most sensitive to O. basilicum and L. multiflora EOs while essential oil of A. conyzoides showed the highest activity on SF-763 cells. Altogether these results justify the use of these plants in traditional medicine in Burkina Faso and open a new field of investigation in the characterization of the molecules involved in anti-proliferative processes. © 2014 Bayala et al.

Chalchat J.,LEXVA ANALYTIQUE | Ozcan M.,Selcuk University | Figueredo G.,LEXVA ANALYTIQUE | Chalard P.,Laboratoire Of Chimie Des Heterocycles Et Des Glucides
Acta Botanica Hungarica | Year: 2011

The essential oils of leaves of Echinophora tenuifolia subsp. sibthorpiana (Apiaceae) growing wild in Middle Anatolia were obtained by hydrodistillation, and analysed by gas chromatography (GC) and gas chromatography-mass spectrometer (GC-MS). The major constituents were affected depend on harvest years. Methyl-eugenol, δ-3-carene and p-cymene were established as the major components of pickling herb leaves collected between 2003 and 2009. The main constituents of leave oil were methyl-eugenol (24.99% to 90.16%), δ-3-carene (2.57% to 34.80%) and p-cymene (1.23% to 9.81%) depending on the harvest years.

Figueredo G.,Lexva Analytique | Chalchat J.C.,Lexva Analytique | Al Juhaimi F.Y.,King Saud University | Ozcan M.M.,Selcuk University
Asian Journal of Chemistry | Year: 2012

The essential oils of leave of Foeniculum vulgare subsp. piperitum (Apiaceae) growing wild in south Anatolia, Turkey were extracted by hydrodistillation and analyzed by GC and GC-MS. The percentage yields of the essential oils from leaves of bitter fennel harvested in different years (from 2003 to 2008) were 1.3, 3.09, 2.1, 1.95, 1.7 and 1.9 %, respectively. The major constituents varied depending on harvest years. Methyl chavicol, fenchone, limonene and fenchyl acetate (exo) were established as the major components of bitter fennel leaves collected in different years. The main constituents of leave oil were methyl chavicol (24.67-69.93 %), fenchone (7.82-14.50 %), limonene (2.39-9.85 %) and fenchyl acetate (exo) (0.17-14.21 %) among the years.

Lograda T.,Ferhat Abbas University Setif | Ramdani M.,Ferhat Abbas University Setif | Chalard P.,University Blaise Pascal | Figueredo G.,LEXVA Analytique | And 2 more authors.
Journal of Applied Pharmaceutical Science | Year: 2013

The chemical composition of essential oil isolated from Chrysanthemum fontanesii and C. coronarium by hydrodistillation, was analysed by GC and GC/MS. A total 66 compounds representing 97.9% of the oil were identified in C. fontanesii, and 44 components representing 97.2% of the total oil in C. coronarium. The chemical composition of C. fontanesii and C. coronarium, is very different, the only common components are α-pinene, β- pinene, myrcene and δ3-carene. The Essential oil of C. fontanesii and C. coronarium was tested for antibacterial activity against nine bacteria strains. The oil showed modest effect against Staphylococcus aureus ATCC 25923, and it has no significant antibacterial activity against the other bacteria tested. The population of C. fontanesii studied showed a diploid chromosome number 2n = 2x = 18 and a tetraploid 2n = 4x = 36 for C. coronarium. © 2013 Parvin Babaei and Bahram Soltani Tehrani.

Lograda T.,Ferhat Abbas University Setif | Chaker A.N.,Ferhat Abbas University Setif | Chalchat J.C.,University Blaise Pascal | Ramdani M.,Ferhat Abbas University Setif | And 3 more authors.
Natural Product Communications | Year: 2010

The hydrodistilled oils from the aerial parts of Genista ulicina Spach. and G. vepres Pomel., which are endemic to Algeria, were analyzed by gas chromatography-mass spectrometry (GC-MS). In the oil of G. ulicina, 41 compounds were identified representing 90.8% of the total oil, and in G. vepres, 61 compounds representing 84.5% of the total oil. The analyses showed that the major constituents of the oils were lauric acid (14.3% - 8.5%), myristic acid (11.5% - 5%), linoleic acid (3.1% - 11.7%) and palmitic acid (18.6% - 26.4%). Using a diffusion method, the oils showed significant antibacterial activities against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923).

Ramdani M.,Ferhat Abbas University Setif | Lograda T.,Ferhat Abbas University Setif | Chalard P.,National Graduate School of Chemistry of Clermont Ferrand | Chalchat J.C.,University Blaise Pascal | Figueredo G.,LEXVA Analytique
Natural Product Communications | Year: 2011

Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Central Sahara of Algeria), were analyzed by gas chomatography coupled to mass spectrometry (GC-MS). Analyses were carried out on 164 trees of 26 natural populations in order to determine the intra-specific variability. Thirty-two terpenoïds were identified, the major ones being α-pinene (11.5-44.2), Δ3-carene (5.7-31.7) and germacrene-D (15.7-54.1). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variability confirmed that genetic factors were not responsible for the decrease in the number of this species, the main reason probably being the Tassili n'Ajjer desertification.

Figueredo G.,Lexva Analytique | Unver A.,Selcuk University | Chalchat J.C.,Lexva Analytique | Arslan D.,Selcuk University | Ozcan M.M.,Selcuk University
Journal of Food Biochemistry | Year: 2012

Chemical compositions of essential oils obtained by hydrodistillation and microwave extraction systems from sage (Salvia fruticosa Mill.), rosemary (Rosmarinus officinalis L.), basil (Ocimum basilicum L.), summer savory (Satureja hortensis L.), winter savory (Satureja thymbra L.) and mint (Mentha spicata L.) were determined with gas chromatography and gas chromatography-mass spectrometry. Carvacrol (38.72 and 34.03%), thymol (17.15 and 14.36%) and p-cymene (12.26 and 18.32%) were determined in high concentrations in the savory oil extracted by both microwave and hydrodistillation extraction systems. The most important ones for rosemary were 1,8-cineole, camphene and borneol. The main components of essential oil of sage obtained by both methods were 52.96 and 51.25% 1,8-cineole, 3.11 and 4.41% alfa-pinene. The major components in essential oil of mint were determined as 80.63 and 68.46% carvone, in decreasing order. The dominant components of essential oil of basil obtained by both microwave distillation or clevenger distillation method were; 30.44 and 34.90% linalool, 25.48 and 31.40% methyl chavicol, and 19.31 and 13.19% (E)-methyl cinnamate. The major components of Satureja hortensis were identified as p-cymene (10.95 and 19.93%), γ-terpinene (8.33 and 20.53%) and carvacrol (59.35 and 38.83%). © 2011 Wiley Periodicals, Inc.

Kossouoh C.,University Abomey Calavi | Moudachirou M.,University Abomey Calavi | Adjakidje V.,University Abomey Calavi | Chalchat J.-C.,University Blaise Pascal | Figueredo G.,LEXVA Analytique
Journal of Essential Oil Research | Year: 2010

The steam-distilled essential oils from fresh leaves and fruits of wild Hyptis suaveolens (L.) Poit. were obtained with a very low percentage and analyzed by GC and GC/MS. Sixty-five compounds were identified. The main components of the oils were β-caryophyllene (43.7%), trans-α- bergamotene (6.3%), caryophyllene oxide (4.4%), 6-hydroxycarvotanacetone (4.4%), bicyclogermacrene (4.2%), (Z)-frans-α-bergamotol (3.9%), terpinen-4-ol (3.5%). © 2010 Allured Business Media.

Traore N.,University Of Bamako | Sidibe L.,University Of Bamako | Figueredo G.,LEXVA Analytique | Chalchat J.-C.,AVAHEA | Chalchat J.-C.,University Blaise Pascal
Journal of Essential Oil Research | Year: 2010

The chemical composition of essential oils isolated from leaves of Eucalyptus houseana, E. citriodora, E. raveretiana, E. robusta and E. urophylla collected at N'Débougou (Mali) was determined by gas chromatography (GC) and GC coupled with mass spectrometry (GC/MS). Sixty-seven compounds were identified. The main constituents were α-pinene (1.3-23.9%), p-cymene (0.3-30.6%), 1,8-cineole (12.3-32.1%), γ-terpinene (0.2-22.3%) and citronellal, present only in E. citriodora (76.3%). © 2010 Allured Business Media.

Type: Journal Article | Journal: Natural product research | Year: 2012

The essential oils of sage leaves (Salvia aucheri Bentham var. canescens Boiss. & Heldr.), growing wild in South Anatolia, were extracted by hydrodistillation and analysed by GC and GC-MS. The percentage yields of the essential oils from sage leaves harvested at different years were 1.0%, 1.3%, 1.3%, 1.0%, 1.4%, 1.5% and 1.2%, respectively. In this study, 1,8-cineole, camphre, camphene -pinene and -pinene were identified as the major components of sage leaves collected at different periods. The main constituents of sage oil collected over the years were 1,8-cineole (35.01-48.06%), camphre (13.58-23.92%), camphene (6.77-8.82%), -pinene (5.79-8.54%) and -pinene (4.32-6.28%).

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