Martinsried, Germany
Martinsried, Germany
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The present invention, inter alia, relates to a closed sterilized container comprising at least one carrier which is a stabilizer; and at least one biomolecule reversibly attached to the carrier, wherein said carrier partially or completely covers the attached biomolecules and wherein said at least one carrier is selected from the group consisting of (poly)peptides such as dipeptides or tripeptides, amino acids, polyalcohols, polyethyleneglycols, ionic liquids, compatible solutes, saponins and a mixture thereof. The invention also relates to methods for producing sterilized containers according to the invention and uses thereof.


Grant
Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: HEALTH.2012.1.2-1 | Award Amount: 8.32M | Year: 2012

Chemotherapy is slowly being supplemented by a new generation of drugs that recognize specific targets in or on cancer cells and has proven to be more effective with markedly fewer side effects. During the course of the disease alternative oncogenic signaling pathways take over inevitably leading to drug resistance. As a consequence renewed tumor analysis is required to redefine the optimal treatment regiment. However a biopsy can frequently not be obtained without risk and or discomfort to the patient. Circulating tumor cells (CTC) may circumvent this problem. CTC refer to cells that detach from a primary tumor or metastatic site, circulate in the peripheral blood and may form metastasis. CTC represent a liquid biopsy that can be used to tailor treatment for individual patients. CTC are however rare and can only be obtained for further characterization in a small fraction of patients. In the CTCtrap consortium universities, research institutions and SMEs are linked in a common effort, starting from the simple, but innovative view of using Therapeutic Apheresis (TA), as a way to collect CTC from peripheral blood in cancer patients. A new TA column will be developed to capture CTC and then reintroduce the blood devoid of tumor cells back into the body with the promise to obtain CTC in all patients at risk for recurrence or diagnosed with metastatic disease. The molecular characterization of these CTC is expected to gather new knowledge on metastasis mechanism, provide a risk assessment and the optimal therapy choice during the course of the disease of cancer patients. The new knowledge on CTC heterogeneity within cancer type and within individuals will allow for the tuning of CTCapheresis to specific cancer types. Prospective pilot studies will be setup to investigate the feasibility of the CTCapheresis in the clinic and their potential therapeutic benefit. Success of CTCapheresis will lead to a radical change in the diagnosis and treatment of solid tumors.


Patent
Leukocare | Date: 2013-08-28

The present invention relates to a kit comprising a blood filter for the production of plasma or serum from a blood sample and a syringe, the blood filter comprising a filter membrane having opposite first and second sides, a receiving compartment defining a hollow space for receiving a blood sample to be filtered from said syringe, said receiving compartment having at least one opening covered with said filter membrane, wherein said first side is facing the receiving compartment, and wherein the hollow space of said receiving compartment has a first volume; and a sampling compartment being arranged on said second side of said filter membrane; wherein the syringe is adapted to receive a predetermined second volume of blood sample, and wherein the first volume is 3 to 20 times larger than the second volume.


The present invention, inter alia, relates to a closed sterilized container comprising at least one carrier which is a stabilizer; and at least one biomolecule reversibly attached to the carrier, wherein said carrier partially or completely covers the attached biomolecules and wherein said at least one carrier is selected from the group consisting of (poly)peptides such as dipeptides or tripeptides, amino acids, polyalcohols, polyethyleneglycols, ionic liquids, compatible solutes, saponins and a mixture thereof. The invention also relates to methods for producing sterilized containers according to the invention and uses thereof.


Patent
Leukocare | Date: 2012-06-28

The present invention relates to a method for stabilising viruses or bacteria, the method comprising embedding the viruses or bacteria in an aqueous solution, wherein the solution comprises: (i) at least three different amino acids; or (ii) at least one dipeptide or tripeptide and wherein the solution is free or substantially free of sugar(s), silanes and protein(s).


The present invention relates to a method for preventing the unfolding of a (poly)peptide during drying and/or inducing the (re-)folding of a (poly)peptide after drying, comprising the step of embedding the (poly)peptide in an aqueous solution, wherein the solution comprises (i) at least three different amino acids; or (ii) at least one dipeptide or tripeptide; and wherein the solution is free or substantially free of (a) sugar; and (b-i) protein; and/or (b-ii) denaturing compounds; and (c) silanes.


Patent
Leukocare | Date: 2010-03-31

The present invention relates to the use of a composition comprising (a) at least three different amino acids, (b) at least two different amino acids and a saponin or (c) at least one dipeptide or tripeptide for stabilizing biomolecules immobilized on a solid carrier. The invention furthermore relates to a method for producing stabilized biomolecules, comprising embedding the biomolecules in the composition according to the invention and a method of producing a solid carrier having biomolecules attached thereto. The invention furthermore relates to a solid carrier producible or produced by the method of the invention and a method of diagnosing a disease using the carrier of the invention.


The present invention relates to a method for preventing the unfolding of a (poly)peptide during drying and/or inducing the (re-)folding of a (poly)peptide after drying, comprising the step of embedding the (poly)peptide in an aqueous solution, wherein the solution comprises (i) at least three different amino acids; or (ii) at least one dipeptide or tripeptide; and wherein the solution is free or substantially free of (a) sugar; and (b-i) protein; and/or (b-ii) denaturing compounds; and (c) silanes.


Patent
Leukocare | Date: 2010-06-17

The present invention relates to a method for filtering blood to produce plasma or serum and to a blood filter for the production of plasma or serum from a blood sample. The invention further relates to a kit comprising a blood filter for the production of plasma or serum from a blood sample and a syringe.


The present invention relates to a method of producing a solid carrier, to which at least one affinity-reagent is coupled that binds specifically to a target molecule, comprising the steps of (i) coupling at least one affinity-reagent to a solid carrier, (ii) overlaying the affinity-reagent-coupled carrier obtained in (i) by incubation with a composition comprising heparin and/or a functional derivative thereof, and (iii) overlaying the affinity-reagent-coupled carrier by incubation with a composition comprising stabilising excipients, wherein steps (ii) and (iii) are carried out subsequently or simultaneously and subsequently to step (i). The invention further relates to a solid carrier comprising (i) at least one affinity-reagent, wherein the at least one affinity-reagent is coupled to the solid carrier, (ii) an overlay comprising heparin and/or a functional derivative thereof, and (ii) an overlay comprising stabilising excipients. In addition, the invention relates to the use of the carrier of the invention or of the carrier produced by the method of the invention to specifically bind and enrich a target molecule or a target cell which carries a biological target molecule on its surface from a liquid sample. Further, the invention relates to the use of the carrier of the invention or of the carrier produced by the method of the invention to specifically induce a biological response in a target cell. Furthermore, the invention relates to an ex-vivo or in-vitro method for specifically binding and enriching a target cell out of a liquid sample comprising contacting the liquid sample with the carrier of the invention or the carrier produced by the method of the invention, wherein the contacting is effected by inducing the liquid sample to flow over the surface of the carrier to which the at least one affinity-reagent is coupled, and wherein the target cell carries a biological target molecule on its surface to which the at least one affinity-reagent specifically binds. Moreover, the invention relates to the carrier of the invention or the carrier produced by the methods of the invention for use as an implant or as an extracorporeal device.

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