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Asnières-sur-Seine, France
Asnières-sur-Seine, France

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Ferey L.,Chimie Paristech | Ferey L.,Agro ParisTech | Ferey L.,French National Institute for Agricultural Research | Delaunay N.,Chimie Paristech | And 10 more authors.
Talanta | Year: 2014

For the first time, the separation of 19 polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants in environmental and food samples by the United States Environmental Protection Agency (US-EPA) and the European Food Safety Authority was developed in cyclodextrin (CD)-modified capillary zone electrophoresis with laser-induced fluorescence detection (excitation wavelength: 325 nm). The use of a dual CD system, involving a mixture of one neutral CD and one anionic CD, enabled to reach unique selectivity. As solutes were separated based on their differential partitioning between the two CDs, the CD relative concentrations were investigated to optimize selectivity. Separation of 19 PAHs with enhanced resolutions as compared with previous studies on the 16 US-EPA PAHs and efficiencies superior to 1.5×10 5 were achieved in 15 min using 10 mM sulfobutyl ether-β-CD and 20 mM methyl-β-CD. The use of an internal standard (umbelliferone) with appropriate electrolyte and sample compositions, rinse sequences and sample vial material resulted in a significant improvement in method repeatability. Typical RSD variations for 6 successive experiments were between 0.8% and 1.7% for peak migration times and between 1.2% and 4.9% for normalized corrected peak areas. LOQs in the low μg/L range were obtained. For the first time in capillary electrophoresis, applications to real vegetable oil extracts were successfully carried out using the separation method developed here. © 2013 Elsevier B.V.


Harbeby E.,French National Institute for Agricultural Research | Jouin M.,French National Institute for Agricultural Research | Alessandri J.-M.,French National Institute for Agricultural Research | Lallemand M.-S.,French National Institute for Agricultural Research | And 5 more authors.
Prostaglandins Leukotrienes and Essential Fatty Acids | Year: 2012

n-3 Polyunsaturated fatty acids (PUFA) support whole brain energy metabolism but their impact on neuroenergetics in specific brain areas and during neuronal activation is still poorly understood. We tested the effect of feeding rats as control, n-3 PUFA-deficient diet, or docosahexaenoic acid (DHA)-supplemented diet on the expression of key genes in fronto-parietal cortex and hippocampal neuroenergetics before and after neuronal stimulation (activated) by an enriched environment. Compared to control rats, n-3 deficiency specifically repressed GLUT1 gene expression in the fronto-parietal cortex in basal state and also during neuronal activation which specifically stimulated GLUT1. In contrast, in the CA1 area, n-3 deficiency improved the glutamatergic synapse function in both neuronal states (glutamate transporters, Na +/K + ATPase). DHA supplementation induced overexpression of genes encoding enzymes of the oxidative phosphorylation system and the F1F0 ATP synthase in the CA1 area. We conclude that n-3 deficiency repressed GLUT1 gene expression in the cerebral cortex, while DHA supplementation improved the mitochondrial ATP generation in the CA1 area of the hippocampus. © 2012 Elsevier Ltd.


Harbeby E.,French National Institute for Agricultural Research | Pifferi F.,CNRS Mechanical Adaptation and Evolution | Jouin M.,French National Institute for Agricultural Research | Pelerin H.,French National Institute for Agricultural Research | And 6 more authors.
OCL - Oleagineux Corps Gras Lipides | Year: 2012

The content of docosahexaenoic acid (DHA) in brain membranes is of crucial importance for the optimum development of brain functions. A lack of DHA accretion in the brain is accompanied by deficits in learning behavior linked to impairments in neurotransmission processes, which might result from alteration of brain fuel supply and hence energy metabolism. Experimental data we published support the hypothesis that n-3 fatty acids may modulate brain glucose utilization and metabolism. Indeed rats made deficient in DHA by severe depletion of total n-3 fatty acid intake have 1) a lower brain glucose utilization, 2) a decrease of the glucose transporter protein content GLUT1 both in endothelial cells and in astrocytes, 3) a repression of GLUT1 gene expression in basal state as well as upon neuronal activation. This could be due to the specific action of DHA on the regulation of GLUT1 expression since rat brain endothelial cells cultured with physiological doses of DHA had an increased GLUT1 protein content and glucose transport when compared to non-supplemented cells. These experimental data highlight the impact of n-3 fatty acids on the use of brain glucose, thereby constituting a key factor in the control of synaptic activity. This emerging role suggests that dietary intake of n-3 fatty acids can help to reduce the cognitive deficits in the elderly and possibly symptomatic cerebral metabolic alterations in Alzheimer disease by promoting brain glucose metabolism.


Ferey L.,Chimie Paristech | Ferey L.,ESPCI ParisTech | Ferey L.,French National Institute for Agricultural Research | Ferey L.,CNRS Physical Chemistry of Electrolytes and Interfacial Nanosystems | And 13 more authors.
Journal of Chromatography A | Year: 2013

Polycyclic aromatic hydrocarbons (PAHs) are among the most targeted contaminants by international regulatory institutions. There is thus a need for fast, selective and sensitive analytical methods to quantify these compounds at trace levels in complex samples. This article focuses on the optimization by means of an experimental design of a CE method with laser-induced fluorescence detection for the fast simultaneous separation of 8 heavy PAHs among food and environmental priority pollutants: benzo(a)pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, dibenzo(a,h)anthracene, indeno(1,2,3-cd)pyrene, benzo(k)fluoranthene, and benzo(ghi)perylene. In this method, capillary zone electrophoresis with a mixture of an anionic sulfobutyl ether-β-cyclodextrin (SBE-β-CD) and a neutral methyl-β-cyclodextrin (Me-β-CD) was used to separate PAHs, on the basis of their differential distribution between the two CDs. First, the factors most affecting PAH electrophoretic behavior were identified: SBE-β-CD and Me-β-CD concentrations and percentage of methanol added to the background electrolyte. Then, a response surface strategy using a central composite design was carried out to model the effects of the selected factors on the normalized migration times. To optimize the separation, desirability functions were applied on modeled responses: normalized migration time differences between peak end and peak start of two consecutive peaks, and overall analysis time. From the model, predicted optimum conditions were experimentally validated and full resolution of all 8 PAHs was achieved in less than 7. min using a borate buffer composed of 5.3. mM SBE-β-CD, 21.5. mM Me-β-CD and 10.3% MeOH. This CE separation method was successfully applied to real edible oil analysis. © 2013 Elsevier B.V.


Goncalves A.,French National Institute for Agricultural Research | Goncalves A.,French Institute of Health and Medical Research | Goncalves A.,Aix - Marseille University | Roi S.,French National Institute for Agricultural Research | And 13 more authors.
Food Chemistry | Year: 2014

The interactions occurring at the intestinal level between the fat-soluble vitamins A, D, E and K (FSVs) are poorly documented. We first determined each FSV absorption profile along the duodenal-colonic axis of mouse intestine to clarify their respective absorption sites. We then investigated the interactions between FSVs during their uptake by Caco-2 cells. Our data show that vitamin A was mostly absorbed in the mouse proximal intestine, while vitamin D was absorbed in the median intestine, and vitamin E and K in the distal intestine. Significant competitive interactions for uptake were then elucidated among vitamin D, E and K, supporting the hypothesis of common absorption pathways. Vitamin A also significantly decreased the uptake of the other FSVs but, conversely, its uptake was not impaired by vitamins D and K and even promoted by vitamin E. These results should be taken into account, especially for supplement formulation, to optimise FSV absorption. © 2014 Elsevier Ltd. All rights reserved.


Gladine C.,Clermont University | Gladine C.,French National Institute for Agricultural Research | Combe N.,French National Center for Scientific Research | Vaysse C.,French National Center for Scientific Research | And 8 more authors.
Journal of Nutritional Biochemistry | Year: 2013

Rapeseeds are naturally rich in cardioprotective micronutrients but refining leads to substantial losses or the production of undesirable compounds. The Optim'Oils European project proposed innovative refining conditions to produce an optimized rapeseed oil enriched in micronutrients and low in trans linolenic acid. We aimed to investigate cardioprotective properties of this Optimized oil. In a randomized, double-blind, controlled, cross-over study, 59 healthy normolipidaemic men consumed either Optimized or Standard rapeseed oils (20. g/d) and margarines (22. g/d) for 3. weeks. The Optimized oil reduced the trans FA concentration (p = 0.009) and increased the contents of alpha-tocopherol (p = 0.022) and coenzyme Q10 (p < 0.001) in comparison with the Standard oil. Over the 3-week trial, Total-/HDL-cholesterol and LDL-/HDL-cholesterol were increased by 4% (p < 0.05) with the Standard oil consumption whereas none of them rose with the Optimized rapeseed oil which increased the HDL-cholesterol and ApoA1 plasma content (+. 2%, NS and +. 3%, p < 0.05 respectively). The effects observed on the plasma HDL-cholesterol levels (p = 0.059), the Total-/HDL-cholesterol ratio (p = 0.092), and on the ApoA1 concentrations (p = 0.060) suggest an improvement of the cholesterol profile with the Optimized rapeseed oil. Finally, the Optimized oil reduced the plasma content of LDLox (-6%, NS), this effect being significantly different from the Standard oil (p = 0.050). In conclusion, reasonable intake of an Optimized rapeseed oil resulting from innovative refining processes and enriched in cardioprotective micronutrients represent a relevant nutritional approach to prevent the risk of cardiovascular diseases by improving the cholesterol profile and reducing LDL oxidation. © 2013 Elsevier Inc.


Ferey L.,Sofiproteol | Ferey L.,French National Institute for Agricultural Research | Ferey L.,Chimie Paristech | Ferey L.,ESPCI ParisTech | And 14 more authors.
Analytica Chimica Acta | Year: 2014

Because of their high toxicity, international regulatory institutions recommend monitoring specific polycyclic aromatic hydrocarbons (PAHs) in environmental and food samples. A fast, selective and sensitive method is therefore required for their quantitation in such complex samples. This article deals with the optimization, based on an experimental design strategy, of a cyclodextrin (CD) modified capillary zone electrophoresis separation method for the simultaneous separation of 19 PAHs listed as priority pollutants. First, using a central composite design, the normalized peak-start and peak-end times were modelled as functions of the factors that most affect PAH electrophoretic behavior: the concentrations of the anionic sulfobutylether-β-CD and neutral methyl-β-CD, and the percentage of MeOH in the background electrolyte. Then, to circumvent computational difficulties resulting from the changes in migration order likely to occur while varying experimental conditions, an original approach based on the systematic evaluation of the time intervals between all the possible pairs of peaks was used. Finally, a desirability analysis based on the smallest time interval between two consecutive peaks and on the overall analysis time, allowed us to achieve, for the first time in CE, full resolution of all 19 PAHs in less than 18. min. Using this optimized capillary electrophoresis method, a vegetable oil was successfully analyzed, proving its suitability for real complex sample analysis. © 2014 Elsevier B.V.


PubMed | Lesieur and French National Institute for Agricultural Research
Type: Journal Article | Journal: European journal of nutrition | Year: 2015

The aim of this study was to evaluate and compare the musculoskeletal effects induced by ovariectomy-related fat mass deposition against the musculoskeletal effects caused by a high-fat diet.A group of adult female rats was ovariectomized and fed a control diet. Two additional groups were sham-operated and fed a control or a high-fat diet for 19 weeks. Distal femur and serum bone parameters were measured to assess bone metabolism. Muscle protein metabolism, mitochondrial markers and triglyceride content were evaluated in tibialis anterior. Triglyceride content was evaluated in liver. Circulating inflammatory and metabolic markers were determined.The high-fat diet and ovariectomy led to similar increases in fat mass (+36.6-56.7%; p < 0.05) but had different impacts on bone and muscle tissues and inflammatory markers. Consumption of the high-fat diet led to decreased bone formation (-38.4%; p < 0.05), impaired muscle mitochondrial metabolism, muscle lipotoxicity and a 20.9% increase in tibialis anterior protein synthesis rate (p < 0.05). Ovariectomy was associated with higher bone turnover as bone formation increased +72.7% (p < 0.05) and bone resorption increased +76.4% (p < 0.05), leading to bone loss, a 17.9% decrease in muscle protein synthesis rate (p < 0.05) and liver lipotoxicity.In female rats, high-fat diet and ovariectomy triggered similar gains in fat mass but had different impacts on bone and muscle metabolism. The ovariectomy-induced mechanisms affecting the musculoskeletal system are mainly caused by estrogen depletion, which surpasses the potential-independent effect of adiposity.


PubMed | Lesieur, Chimie Paristech, French National Institute for Agricultural Research, ESPCI ParisTech and Sofiproteol
Type: | Journal: Analytica chimica acta | Year: 2014

Because of their high toxicity, international regulatory institutions recommend monitoring specific polycyclic aromatic hydrocarbons (PAHs) in environmental and food samples. A fast, selective and sensitive method is therefore required for their quantitation in such complex samples. This article deals with the optimization, based on an experimental design strategy, of a cyclodextrin (CD) modified capillary zone electrophoresis separation method for the simultaneous separation of 19 PAHs listed as priority pollutants. First, using a central composite design, the normalized peak-start and peak-end times were modelled as functions of the factors that most affect PAH electrophoretic behavior: the concentrations of the anionic sulfobutylether--CD and neutral methyl--CD, and the percentage of MeOH in the background electrolyte. Then, to circumvent computational difficulties resulting from the changes in migration order likely to occur while varying experimental conditions, an original approach based on the systematic evaluation of the time intervals between all the possible pairs of peaks was used. Finally, a desirability analysis based on the smallest time interval between two consecutive peaks and on the overall analysis time, allowed us to achieve, for the first time in CE, full resolution of all 19 PAHs in less than 18 min. Using this optimized capillary electrophoresis method, a vegetable oil was successfully analyzed, proving its suitability for real complex sample analysis.


Dhaussy A.,LESIEUR
OCL - Oilseeds and fats, crops and lipids | Year: 2014

The vitamin D has recently been the subject of many researches, demonstrating new effects, reinforcing the interest for this molecule. The interest to develop products fortified with vitamin D is thus very important. Regarding references values, it is necessary to distinguish the RDAs (Recommended Daily Allowances), single value set up at 5 μg/d for vitamin D at the European level, in order to be a reference and a labeling value, from the nutritional recommendations. These recommendations are defined by groups of population. The values vary according to the countries and the dates on which they were set, the most recent being usually higher. The vitamin D fortification is less constrained in France today than before 2006. To fortify a food with vitamin D, it is necessary to follow the rules set by the Regulation 1925/2006/EC, and use the formulations authorized, demonstrate the nutritional benefit of the fortification, and ensure that the fortification does not lead to a risk for the health of the consumers. In some countries, the vitamin D fortification has been made mandatory for some foods. In France, several products are fortified with vitamin D, especially dairy products, but also some breakfast cereals and vegetable oils. Finally, the communication that can be made on vitamin D in food or advertising is strictly regulated by the Regulation 1924/2006/EC. © A. Dhaussy, published by EDP Sciences, 2014.

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