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Rodriguez-Lazaro D.,University of Burgos | Cook N.,UK Environment Agency | Hernandez M.,Leon Institute of Technology
Current Issues in Molecular Biology | Year: 2013

A principal consumer demand is a guarantee of the safety and quality of food. The presence of foodborne pathogens and their potential hazard, the use of genetically modified organisms (GMOs) in food production, and the correct labelling in foods suitable for vegetarians are among the subjects where society demands total transparency. The application of controls within the quality assessment programmes of the food industry is a way to satisfy these demands, and is necessary to ensure efficient analytical methodologies are possessed and correctly applied by the Food Sector. The use of real-time PCR has become a promising alternative approach in food diagnostics. It possesses a number of advantages over conventional culturing approaches, including rapidity, excellent analytical sensitivity and selectivity, and potential for quantification. However, the use of expensive equipment and reagents, the need for qualified personnel, and the lack of standardized protocols are impairing its practical implementation for food monitoring and control.

Agency: Cordis | Branch: H2020 | Program: RIA | Phase: LCE-11-2015 | Award Amount: 5.99M | Year: 2016

WASTE2FUELS aims to develop next generation biofuel technologies capable of converting agrofood waste (AFW) streams into high quality biobutanol. Butanol is one of the most promising biofuels due to its superior fuel properties compared to current main biofuels, bioethanol and biodiesel. In addition to its ability to reduce carbon emissions, its higher energy content (almost 30% more than ethanol), its ability to blend with both gasoline and diesel, its lower risk of separation and corrosion, its resistance to water absorption, allowing it to be transported in pipes and carriers used by gasoline, it offers a very exciting advantage for adoption as engines require almost no modifications to use it. The main WASTE2FUELS innovations include: Development of novel pretreatment methods for converting AFW to an appropriate feedstock for biobutanol production thus dramatically enlarging current available biomass for biofuels production Genetically modified microorganisms for enhancing conversion efficiencies of the biobutanol fermentation process Coupled recovery and biofilm reactor systems for enhancing conversion efficiencies of Acetone-Butanol-Ethanol fermentation Development of new routes for biobutanol production via ethanol catalytic conversion Biobutanol engine tests and ecotoxicological assessment of the produced biobutanol Valorisation of the process by-products Development of an integrated model to optimise the waste-to-biofuel conversion and facilitate the industrial scale-up Process fingerprint analysis by environmental and techno-economic assessment Biomass supply chain study and design of a waste management strategy for rural development By valorising 50% of the unavoidable and undervalorised AFW as feedstock for biobutanol production, WASTE2FUELS could divert up to 45 M tonnes of food waste from EU landfills, preventing 18 M tonnes of GHG and saving almost 0.5 billion litres of fossil fuels.

Rodriguez-Lazaro D.,University of Burgos | Hernandez M.,Leon Institute of Technology
Current Issues in Molecular Biology | Year: 2013

Food safety and quality control programmes are increasingly applied throughout the production food chain in order to guarantee added value products as well as to minimize the risk of infection for the consumer. The development of real-time PCR has represented one of the most significant advances in food diagnostics as it provides rapid, reliable and quantitative results. These aspects become increasingly important for the agricultural and food industry. Different strategies for real-time PCR diagnostics have been developed including unspecific detection independent of the target sequence using fluorescent dyes such as SYBR Green, or by sequence-specific fluorescent oligonucleotide probes such as TaqMan probes or molecular beacons.

Avila M.,Instituto Nacional Of Investigacion Y Tecnologia Agraria Y Alimentaria Inia | Gomez-Torres N.,Instituto Nacional Of Investigacion Y Tecnologia Agraria Y Alimentaria Inia | Hernandez M.,Leon Institute of Technology | Garde S.,Instituto Nacional Of Investigacion Y Tecnologia Agraria Y Alimentaria Inia
International Journal of Food Microbiology | Year: 2014

The butyric acid fermentation, responsible for late blowing of cheese, is caused by the outgrowth in cheese of some species of Clostridium, resulting in texture and flavor defects and economical losses. The aim of this study was to evaluate the effectiveness of different antimicrobial compounds against vegetative cells and spores of C. tyrobutyricum, C. butyricum, C. beijerinckii and C. sporogenes strains isolated from cheeses with late blowing defect. Minimal inhibitory concentration (MIC) for reuterin, nisin, lysozyme and sodium nitrite were determined against Clostridium strains in milk and modified RCM (mRCM) after 7. d exposure. Although the sensitivity of Clostridium to the tested antimicrobials was strain-dependent, C. sporogenes and C. beijerinckii generally had higher MIC values than the rest of Clostridium species. The majority of Clostridium strains were more resistant to antimicrobials in milk than in mRCM, and vegetative cells exhibited higher sensitivity than spores. Reuterin (MIC values 0.51-32.5. mM) and nisin (MIC values 0.05-12.5. μg/ml) were able to inhibit the growth of vegetative cells and spores of all assayed Clostridium strains in milk and mRCM. Strains of C. tyrobutyricum exhibited the highest sensitivity to lysozyme (MIC values. <. 0.20-400. μg/ml) and sodium nitrite (MIC values 18.75-150. μg/ml). These results suggest that reuterin and nisin, with a broad inhibitory activity spectrum against Clostridium spp. spores and vegetative cells, may be the best options to control Clostridium growth in dairy products and to prevent associated spoilage, such as late blowing defect of cheese. However, further studies in cheese would be necessary to validate this hypothesis. © 2013 Elsevier B.V.

Agency: Cordis | Branch: FP7 | Program: CP-IP | Phase: KBBE-2007-2-4-05 | Award Amount: 6.75M | Year: 2009

Food Safety Objectives (FSO) and Performance Objectives (PO) are new criteria complementing the existing concepts of microbiological criteria and MRL for many chemical contaminants. However, to achieve these objectives it is critically important a harmonisation of food safety control procedures. BASELINE project intends to obtain the following objectives: 1) To review the sampling schemes currently available for food authorities and food producers to perform food safety quantitative risk assessment in a European level; 2) To assess the relevance and suitable limit values of POs and FSOs for biological and chemical risks; 3) To evaluate the need for new or adapted methods for sampling and testing of the risk factors identified. The selected protocols and methods should be able to produce suitable data for risk analysis; 4) To develop predictive mathematical models for biological risks and investigate and model sources and pathways of chemical contaminants to improve sampling schemes; 5) To validate and harmonise the sampling schemes developed in the project and alternative detection methods; 6) To share and disseminate the scientific knowledge deriving from the project to stakeholders. The BASELINE work plan has been divided in 9 work packages: WP1- management, WP2-WP6 sampling protocols for specific food matrixes, WP7-risk modelling, WP8-validation and harmonisation of sampling protocols, WP9-dissemination and training. The major output of the project is to generate new knowledge on sampling schemes for risk assessment by using a mathematical approach for different groups of food products as seafood, eggs and egg products, fresh meats, milk and dairy products and plant products. The project results will be translated in clear recommendations to the EC and end users and they will have a significant impact on protecting human and veterinary health.

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