Lemonex Inc.

Seoul, South Korea

Lemonex Inc.

Seoul, South Korea
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Patent
Lemonex Inc. and Won | Date: 2017-05-31

The present invention relates to a composition for delivering a protein, and a method for delivering a bioactive material or a protein by using the same.


A composition for delivering a bioactive material include a porous silica nanoparticle containing pores with an average pore diameter ranging from 1 nm to 100 nm, at least one of (i) a functional group which binds to the pore surface of the porous silica nanoparticle and gives the pore surface a negative charge or a positive charge, (ii) a ligand which binds to the pore surface of the porous silica nanoparticle and specifically binds to the bioactive material, and (iii) a combination of the functional group and the ligand, and a bioactive material having a size to be accommodated within the pores of the porous silica nanoparticle, the bioactive material bound to said at least one of the functional group and the ligand bound to the pore surface of the mesoporous silica nanoparticle and accommodated within the pores of the porous silica nanoparticle.


Lee J.-S.,Seoul National University | Kim S.,Seoul National University | Min D.-H.,Seoul National University | Min D.-H.,Lemonex Inc.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 | Year: 2016

One aim of the recent researches on photodynamic therapy (PDT) is to overcome the limitation of conventional PDT by improving selective activation of photosensitizer on targeted region. Here, we develop a miRNA-responsive drug release system which explore the role of miRNA as an internal initiator of drug in cancer treatment. We demonstrate that released drug can show the sequencespecific fluorescence signal responding to target miRNA and be activated under near IR (NIR) irradiation generating cytotoxic singlet oxygen for selective PDT. The present study fulfilled an effective nanomedicine enabling imaging-guided photodynamic therapy in vitro and in vivo.


Lee J.,Seoul National University | Park G.,Seoul National University | Min D.-H.,Seoul National University | Min D.-H.,Lemonex Inc.
Chemical Communications | Year: 2015

Graphene oxide quenches fluorescence corresponding to only a mismatched target due to selective denaturing of the thermo-unstable duplex composed of probe peptide nucleic acid and single base mismatched target RNA and thus, the fluorescence signal only from perfectly matched target RNA is measured. © The Royal Society of Chemistry 2015.


Lee J.,Seoul National University | Park I.-S.,Seoul National University | Kim H.,Korea Advanced Institute of Science and Technology | Woo J.-S.,Seoul National University | And 3 more authors.
Biosensors and Bioelectronics | Year: 2015

Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds. © 2015 Elsevier B.V.


Jang H.,Seoul National University | Choi M.-H.,Seoul National University | Yim Y.,Seoul National University | Kim Y.-K.,Seoul National University | And 2 more authors.
Advanced Healthcare Materials | Year: 2015

Herein, hybrid nanocomposite of praseodymium doped TiO2 nanocrystals and graphene oxide nanosheets are prepared by facile hydrothermal treatment. As-synthesized Pr-TiO2/NGO hybrid nanocomposite exhibits enhanced photocatalytic activity under visible light irradiation by the intact graphene oxide and doped lanthanide mediated band gap narrowing compared to TiO2. Moreover, high payload and controlled release of doxorubicin by charge reversal of hybrid nanocomposite at endosomal pH and near-infrared irradiation mediated efficient photothermal conversion provide highly favorable features in therapeutic applications. Through the combination of these three distinctive therapeutic modalities, highly efficient trimodal cancer cell ablation is demonstrated. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Jang H.,Korea Basic Science Institute | Kim D.-E.,Konkuk University | Min D.-H.,Korea Basic Science Institute | Min D.-H.,Lemonex Inc.
ACS Applied Materials and Interfaces | Year: 2015

Hollow Au-Ag bimetallic nanoshell possessing hydrophobic interior space and hydrophilic exterior surface was prepared and its application as a chemo-thermo-gene therapeutic agent based on its high payload of multiple drugs having different water solubility was demonstrated. The multifunctional drug delivery system is based on the hydrophobic interior created by the self-assembled monolayer (SAM) of hexanethiol onto the inner surface of the hollow metallic nanoshells whereas the outer surface was mostly coated by hydrophilic biocompatible polymer. The nanoshells having surface environment modified by hexanethiol SAMs provided high capacity both for hydrophilic DNAzyme (Dz) to induce gene silencing and for hydrophobic SN38 (7-ethyl-10-hydroxycamptothecin), anticancer drug. The release of the loaded Dz and SN38 was independently triggered by an acidic environment and by photothermal temperature elevation upon irradiation, respectively. The chemo-thermo-gene multitherapy based on the present nanoshells having modified surface environment showed high efficacy in quantitative cell-based assays using Huh7 human liver cell containing hepatitis C viral NS3 gene replicon RNA. © 2015 American Chemical Society.

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