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Akhwale J.K.,Leibniz Institute DSMZGerman Collection of Microorganisms and Cell Cultures | Akhwale J.K.,Jomo Kenyatta University of Agriculture and Technology | Goker M.,Leibniz Institute DSMZGerman Collection of Microorganisms and Cell Cultures | Rohde M.,elmholtz Center for Infection Research | And 4 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2016

During a screening for novel and biotechnologically useful bacteria in haloalkaline lakes, strain No.156T was isolated from a sediment sample from lake Elmenteita in the African Rift Valley and studied by a polyphasic taxonomic approach. The strain was observed to form yellow aerial and substrate mycelia; optimal growth was found to be at 30–35 °C in salt concentrations of 6–9 % (w/v) and at pH 7–9. The DNA G+C content of the novel strain was 71 mol%. Analysis of 16S rRNA sequences indicated that the isolate belongs to the genus Nocardiopsis with sequence similarities below 98 % to the type strains of all other representatives of the genus. Mycolic acids were not detected in whole cell methanolysates. The peptidoglycan was found to contain meso-diaminopimelic acid as the diamino acid with no diagnostic sugars. The main polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol but no diphosphatidylglycerol. The predominant menaquinones were MK-11(H8), MK-11(H6), MK-10(H8) and MK-10(H6). Cellular fatty acids were found to consist of saturated and monounsaturated iso- and anteiso-branched acids with 16–18 C-length, tuberculostearic acid (Me18:0), and straight-chain saturated (16:0, 18:0) acids. These characteristics match those of the genus Nocardiopsis. Based on 16S rRNA gene sequence analysis and phenotypic characteristics, a novel species with the name Nocardiopsis mwathae is proposed. The type strain is No.156T (=DSM 46659T = CECT 8552T). The INSDC accession number for the 16S rRNA gene sequence of strain No.156T is KF976731. © 2016 Springer International Publishing Switzerland


Ozdemir-Kocak F.,Bilecik University | Saygin H.,Ondokuz Mayis University | Saricaoglu S.,Ondokuz Mayis University | Cetin D.,Gazi University | And 6 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2016

A novel Gram-stain positive, rod-shaped, non-motile and mycolic acid containing strain, FMN18T, isolated from soil, was characterised using a polyphasic approach. The organism showed a combination of morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia and it formed a phyletic line in the Nocardia 16S rRNA gene tree. The cell wall contained meso-diaminopimelic acid (type IV) and whole cell sugars were galactose, glucose, arabinose and ribose. The predominant menaquinone was MK-8(H4ω-cyclo). The major phospholipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Major fatty acids are C16:0, 10-methyl C18:0 (TBSA), C18:1cis9 and C16:1trans9. These chemotaxonomic traits are in good agreement with those known for representatives of the genus Nocardia. The phylogenetic analysis based on the 16S rRNA gene sequence of strain FMN18T showed it to be closely related to Nocardia grenadensis GW5-5797T (99.2 %), Nocardia speluncae N2-11T (99.1 %), Nocardia jinanensis 04-5195T (99.0 %) and Nocardia rhamnosiphila 202GMOT (98.3 %). The phylogenetic analysis based on the gyrB gene sequence of strain FMN18T showed it to be closely related to N. rhamnosiphila 202GMOT (99.0 %), N. grenadensis DSM 45869T (96.6 %), N. jinanensis DSM 45048T (93.1 %), N. carnea IFM 0237T (89.7 %) and N. speluncae DSM 45078T (89.1 %). A combination of DNA–DNA hybridization results and phenotypic properties demonstrated that strain FMN18T was clearly distinguished from all closely related Nocardia species. It is proposed that the organism be classified as representing a novel species of the genus Nocardia, for which the name Nocardia zapadnayensis (type strain FMN18T = DSM 45872T = KCTC 29234T) is proposed. © 2015, Springer International Publishing Switzerland.


Veyisoglu A.,Sinop University | Carro L.,Northumbria University | Guven K.,Anadolu University | Cetin D.,Gazi University | And 5 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2016

A Micromonospora strain, designated DS3186T, isolated from sediment collected from the Black Sea off the Yason Peninsula, Ordu, Turkey, was examinated using a polyphasic approach. The strain was found to have chemotaxonomic, morphological and phylogenetic properties consistent with its clasification in the genus Micromonospora. A comparative 16S rRNA gene sequence analysis showed that the strain was closely related to the type strains of Micromonospora olivasterospora (99.0 %), Micromonospora equina (98.8 %), Micromonospora rhizosphaerae (98.8 %) and Micromonospora viridifaciens (98.8 %); low levels of DNA–DNA relatednes were found between the isolate and the M. olivasterospora and M. rhizosphaerae strains. Corresponding phylogenetic analysis based on partial gyrB gene sequences showed that strain DS3186T formed a subclade with the type strains of Micromonospora eburnea, M. equina, Micromonospora narathiwatensis and M. viridifaciens. Strain DS3186T was distinguished from its close phylogenetic neighbours using a combination of chemotaxonomic, morphological and physiological properties. Consequently, it is proposed that strain DS3186T represents a novel Micromonospora species for which the name Micromonosporayasonensis sp. nov. is proposed. The type strain is DS3186T (=DSM 45980T = KCTC 29433T). © 2016, Springer International Publishing Switzerland.

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