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Lentini V.,Messina University | Gugliandolo C.,Messina University | Bunk B.,Leibniz Institute DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig | Overmann J.,Leibniz Institute DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig | Maugeri T.L.,Messina University
Current Microbiology | Year: 2014

To investigate the prokaryotic community structure and composition in an active hydrothermal site, named Black Point, off Panarea Island (Eolian Islands, Italy), we examined sediment and fluid samples, differing in temperature, by a massive parallel sequencing (Illumina) technique targeting the V3 region of the 16S rRNA gene. The used technique enabled us to detect a greater prokaryotic diversity than that until now observed and to reveal also microorganisms occurring at very low abundance (B0.01 %). Most of sequences were assigned to Bacteria while Archaea were a minor component of the microbial community in both low- and high-temperature samples. Proteobacteria (mainly consisting of Alpha-, Gamma-, and Epsilonproteobacteria) dominated among all samples followed by Actinobacteria and Bacteroidetes. Analyzed DNA obtained from samples taken at different temperatures indicated the presence of members of different dominant genera. The main differences were observed between sediment samples where Rhodovulum and Thiohalospira prevailed at high temperature, while Thalassomonas and Sulfurimonas at low temperature. Chlorobium, Acinetobacter, Sulfurimonas, and Brevundimonas were abundant in both low- and high-temperature fluid samples. Euryarchaeota dominated the archaeal community in all samples. Classes of Euryarchaeota embracing hyperthermophilic members (Thermococci and Thermoplasmata) and of Crenarchaeota (Thermoprotei) were more abundant in high-temperature samples. A great number of sequences referred to Bacteria and Archaea still remained unaffiliated, indicating that Black Point site represents a rich source of so-far uncharted prokaryotic diversity. © Springer Science+Business Media New York 2014. Source

Chen H.,Ludwig Maximilians University of Munich | Chen H.,Leibniz Institute DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig | Jogler M.,Ludwig Maximilians University of Munich | Jogler M.,Leibniz Institute DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig | And 6 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A novel type of freshwater bacterium was isolated from the prealpine mesotrophic Starnberger See (Bavaria, southern Germany). Cells of strain 382 T were Gram-negative and rod-shaped, motile and creamy-white. The isolate was strictly aerobic, catalase- and oxidase-positive, and grew at pH values of 6-9 (optimum, pH 7) and temperatures of 10-37 °C (optimum, 28 °C). The genomic G+C content of strain 382 T was 64.1 mol%. Based on 16S rRNA gene sequence analyses, strain 382 T belongs to the family Sphingomonadaceae and clusters within the genus Sphingomonas. Sphingomonas histidinilytica UM 2 T and Sphingomonas wittichii DSM 6014 T were the closest relatives, as indicated by the highest 16S rRNA gene sequence similarities (97.1 % and 96.8 %, respectively). Sphingomonas paucimobilis DSM 1098 T (the type species of the genus Sphingomonas) exhibited 95.3 % sequence similarity. This affiliation of strain 382 T to the genus Sphingomonas is confirmed by the presence of Q-10 as the major respiratory quinone, two sphingoglycolipids, C 14: 0 2-OH as the major 2-hydroxy fatty acid and sym-homospermidine as the major polyamine. The main cellular fatty acids of strain 382 T were C 18: 1ω7c (39 %), C 16: 1ω7c (21 %), C 16: 0 (10 %) and C 14: 0 2-OH (10 %). Based on the phylogenetic distance from other species of the genus Sphingomonas and its unusually high C16: 1ω7c content, strain 382 T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas starnbergensis is proposed. The type strain is 382 T (= DSM 25077 T = LMG 26763 T). © 2013 IUMS. Source

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