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Braunschweig, Germany

Kaden R.,National Veterinary Institute SVA | Sproer C.,Leibnitz Institute DSMZ | Beyer D.,Helmholtz Center for Environmental Research | Krolla-Sidenstein P.,Karlsruhe Institute of Technology
International Journal of Systematic and Evolutionary Microbiology

A Gram-stain-negative, oxidase and phosphatase-positive and catalase-negative, short rod-shaped bacterium was isolated from sediment of a drinking water reservoir in Germany. Based on 16S rRNA gene sequence and phenotypic properties, the bacterium belongs to the genus Rhodoferax within the family Comamonadaceae. The new taxon differed from related species mainly with respect to its fatty acid composition, low growth temperature, lack of pigments in young cultures and ability to utilize glycerol and d-mannose but not urea. The major fatty acids were C16: 1ω7c and/or iso-C15: 0 2-OH, C16: 0, and C18: 1ω7c. The only ubiquinone detected was ubiquinone Q-8. The DNA G+C content was 60.3-61 mol%. Because of the phenotypic and genotypic differences from the most closely related taxa, the new strain represents a novel species for which the name Rhodoferax saidenbachensis sp. nov. is proposed. The type strain is ED16T (= CCUG 57711T = ATCC BAA-1852T = DSM 22694T). An emended description of the genus Rhodoferax is proposed. Based on the results of this study, strain T118T (Albidiferax ferrireducens) is properly placed in the genus Rhodoferax as Rhodoferax ferrireducens. © 2014 IUMS. Source

Anderson I.,U.S. Department of Energy | Tindall B.J.,Leibnitz Institute DSMZ | Rohde M.,Helmholtz Center for Infection Research | Lucas S.,U.S. Department of Energy | And 20 more authors.
Standards in Genomic Sciences

Halopiger xanaduensis is the type species of the genus Halopiger and belongs to the euryarchaeal family Halobacteriaceae. H. xanaduensis strain SH-6, which is designated as the type strain, was isolated from the sediment of a salt lake in Inner Mongolia, Lake Shangmatala. Like other members of the family Halobacteriaceae, it is an extreme halophile requiring at least 2.5 M salt for growth. We report here the sequencing and annotation of the 4,355,268 bp genome, which includes one chromosome and three plasmids. This genome is part of a Joint Genome Institute (JGI) Community Sequencing Program (CSP) project to sequence diverse haloarchaeal genomes. Source

Han C.,U.S. Department of Energy | Han C.,Los Alamos National Laboratory | Kotsyurbenko O.,TU Braunschweig | Kotsyurbenko O.,Moscow State University | And 47 more authors.
Standards in Genomic Sciences

Sulfuricurvum kujiense Kodama and Watanabe 2004 is the type species of the monotypic genus Sulfuricurvum, which belongs to the family Helicobacteraceae in the class Epsilonproteobacteria. The species is of interest because it is frequently found in crude oil and oil sands where it utilizes various reduced sulfur compounds such as elemental sulfur, sulfide and thiosulfate as electron donors. Members of the species do not utilize sugars, organic acids or hydrocarbons as carbon and energy sources. This genome sequence represents the type strain of the only species in the genus Sulfuricurvum. The genome, which consists of a circular chromosome of 2,574,824 bp length and four plasmids of 118,585 bp, 71,513 bp, 51,014 bp, and 3,421 bp length, respectively, harboring a total of 2,879 protein-coding and 61 RNA genes and is a part of the Genomic Encyclopedia of Bacteria and Archaea project. Source

Kalfalah F.M.,Heinrich Heine University Dusseldorf | Berg E.,Heinrich Heine University Dusseldorf | Christensen M.O.,Heinrich Heine University Dusseldorf | Linka R.M.,Heinrich Heine University Dusseldorf | And 3 more authors.
Cell Cycle

To maintain genome stability, the thousands of replication origins of mammalian genomes must only initiate replication once per cell cycle. This is achieved by a strict temporal separation of ongoing replication in S phase, and the formation of pre-replicative complexes in the preceding G1 phase, which "licenses" each origin competent for replication. The contribution of the loading factor Cdc6 to the timing of the licensing process remained however elusive due to seemingly contradictory findings concerning stabilization, degradation and nuclear export of Cdc6. Using fluorescently tagged Cdc6 (Cdc6-YFP) expressed in living cycling cells, we demonstrate here that Cdc6-YFP is stable and chromatin-associated during mitosis and G1 phase. It undergoes rapid proteasomal degradation during S phase initiation followed by active export to the cytosol during S and G2 phases. Biochemical fractionation abolishes this nuclear exclusion, causing aberrant chromatin association of Cdc6-YFP and, likely, endogenous Cdc6, too. In addition, we demonstrate association of Cdc6 with centrosomes in late G2 and during mitosis. These results show that multiple Cdc6-regulatory mechanisms coexist but are tightly controlled in a cell cycle-specific manner. © 2015 Taylor & Francis Group, LLC. Source

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