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Bocharova V.,Tairov National Research Center for Viticulture and Winemaking | Mulukina N.,Tairov National Research Center for Viticulture and Winemaking | Kovaliova I.,Tairov National Research Center for Viticulture and Winemaking | Regner F.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | And 2 more authors.
Mitteilungen Klosterneuburg | Year: 2012

A molecular-genetic analysis of polymorphism in grapevine varieties, clones and new selections from the collection of the Tairov National Research Centre for Viticulture and Winemaking was conducted by using 9 microsatellite loci. The data were N-coded and analysed with the application of the MEGA 4 programme in order to build a dendrogram of the genetic relationships. The bootstrap test was used to examine iidelity of the phylogenetic trees. The molecular-genetic polymorphism was investigated using chloroplast DNA markers. Three different haplotypes were identified by cpSSR locus ccmp10.


Maier C.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Maier C.,Austrian Institute of Technology | Bachinger K.,NO Landes Landwirtschaftskammer | Mortel J.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | And 3 more authors.
Journal of Phytopathology | Year: 2013

During the last 15 years, European stone fruit yellows (ESFY) has become a major concern in Austrian fruit production. Therefore, presence and temporal dynamics of its vector Cacopsylla pruni were investigated using a beating tray method and yellow sticky traps on Prunus armeniaca, Prunus domestica, Prunus spinosa and P. cerasifera nigra. Infection rates of C. pruni and Prunus spp. trees were assessed by direct, nested and real-time PCR. Movement of remigrants in a model apricot orchard was tracked by aid of a mark, release and recapture study. Insects were marked by fluorescent dyes. Movement of the marked insects and presence of naturally occurring insects were monitored by yellow sticky traps. In 2011, remigration of C. pruni to Prunus spp. started in calendar week 10 (8th of March) and in 2012, in calendar week 12 (18th of March). Remigrants were observed until calendar week 20 (middle of May), significant numbers of the springtime generation adults were present until week 26 (end of June). The phytoplasma was ascertained in 0-11.5% of the remigrants and in 0-3.44% of the springtime generation insects. About 9.8-63.3% of the apricot samples, 20-40% of the plum samples and single blackthorn samples were infected. The mark, release and recapture study proved a fast and frequent tree-to-tree movement of remigrated C. pruni adults. Insects easily covered distances from row to row or even farther (ca. 13 m) within 24 h after release and were present in a large part of the model orchard after 8 days (up to 24 m from release point). © 2013 Blackwell Verlag GmbH.


Hermann G.,University of Vienna | Jaitz L.,University of Vienna | Koellensperger G.,University of Vienna | Eder R.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Hann S.,University of Vienna
Mitteilungen Klosterneuburg | Year: 2012

Our aim was to verify if quantities in a selected phenolic profile, consisting of 20 phenols, can be used for geographical allocation and varietal specific characterisation of 50 commercially available red wines, with special interest in wines from the region of Vienna. The first task was the development of a fast, reproducible and accurate method for the chromatographic separation of (poly)phenols. The second task was their quantification by means of liquid chromatography coupled to a mass spectrometer (LC-MS) and the third task was data analysis by means of discriminant statistical methods. The used separation method was reversed phase high performance liquid chromatography (RP-HPLC). After the examination of several column materials our choice was a material with a particle size of 1.8 μm. It was superior to other samples regarding the most important characteristics for HPLC separation (capacity factor, height of a theoretical plate and chromatographic resolution). The detection via MS was performed on a time-of-flight and a triple quadrupole mass spectrometer, both equipped with electro spray ionization (ESI) as ionization method. The final measurements were performed with the triple quadrupole mass spectrometer, because of its higher signal to noise ratio and larger linear and dynamic range. The results of the statistical examination confirmed that most wine samples could be grouped properly according to their geographical origin and variety. It was even possible to distinguish between different producers within the region of Vienna.


Gossinger M.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Eitner C.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Vogl K.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg
Mitteilungen Klosterneuburg | Year: 2012

The determination of the point of tails' separation (N-point) works only sufficiently by tasting. The fractionation of the distillate round the N-point is very extensive, mainly if the range, where the N-point appears, is not known. A prediction of the N-point by means of distillation parameters is therefore desirable. Aim of the study was to calculate the effect of mash alcohol content, dephlegmator temperature and distillation speed on the N-point, temperature of the lyne arm just in time of the N-point, the percentage of yield of head, heart and tail fractions and amplification with counter-current distillation of apple mash. For characterising the distillation process properly the average increase of the temperature of the lyne arm and the alcohol content of the distillation fractions were measured. Results show that the N-point (between 85 and 77 %vol.) is affected mainly by the alcohol content of the mash and the temperature of the dephlegmator. Less important is the role of the distillation speed. The higher the alcohol content of the mash and the temperature of the dephlegmator, the lower the percentage of yield of heads. Only the temperature of the dephlegmator had a significant effect on the percentage of yield of heart. Yield increased with intensed amplification. The results are the basis for the calculation of the N-point before or during the distillation.


Riedle-Bauer M.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Mortel J.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Bauer H.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Knobling A.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg
Mitteilungen Klosterneuburg | Year: 2012

Several diagnostic procedures for detection of Agrobacterium vitis in grapevine material were compared. Infected and presumably latently infected vines were analyzed. Roots, tumour and shoot samples were collected all year round. DNA was either gained after an enrichment step on a semiselective medium or extracted directly from grapevine tissue. Standard and nested PCR protocols targeting chromosomal genes and Ti-plasmid borne genes were applied. Direct DNA preparation combined with nested PCR permitted the detection of the pathogen at a high rate in roots, shoots and tumours. The method, however, did not allow detection of bacteria in dormant canes. Th is was only possible by aid of an enrichment step on a selective medium followed by nested PCR. Analysis of infected vines by the latter protocol revealed the presence of the pathogen in 83 to 93 % of tumour samples, in 70 % of the shoot samples collected in autumn and in 80 % of the root samples taken in late spring. During summer low and erratic population densities were observed. In dormant canes the pathogen was identified at a rate of 16.7 to 50 %. An enrichment step followed by nested PCR was also used for examination of symptomless but presumably infected vines. The pathogen was identified ed in 33 to 40 % of the roots collected in spring or autumn and in 20 % of the roots collected in July. Analysis of shoots revealed considerable differences between years. In 2010 A. vitis was identified in 41 to 53 % of the samples, in 2011 only in 0 to 9.1 %.


Mehofer M.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Schmuckenschlager B.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Vitovec N.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Hanak K.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | And 2 more authors.
Mitteilungen Klosterneuburg | Year: 2011

On a site with carbonate containing brown soil on flysch-marl the effect of 31 rootstock varieties on the parameters must weight, acidity of the must, yield, bunch weight and pruning wood weight was determined with the grapevine variety 'Zweigelt'. On the average over the twelve experimental years (1998 to 2009) the rootstocks 41 B, Ru 140 and G 9 caused a significant reduction of the must weight by 0.47 °KMW, 0.26 °KMW and 1.13 °KMW, resp., compared to K 5BB. With the rootstocks 1616 C and 3309 C, however, the must weight was significantly increased by +0.4 °KMW and +0.34 °KMW. The annual mean values of the must weight ranged from a minimum of 16.6 °KMW in 2007 to a maximum of 19.1 °KMW in 2005. The acidity of the must was increased with the rootstocks 41 B, Binova and G 9 by about 0.49 g/l, 0.64 g/l and 0.5 g/l, resp., compared with K 5BB. The lowest annual mean value of titratable acids was 5.4 g/l in 2000 and the highest was 7.8 g/l in 2001 and 2004. The yield was significantly reduced with the rootstocks SO4, Cosmo 10, 99 R, G 9, Aripa, 14 MG, 1616 C, 3309 C, and Riparia Portalis. Binova and 225 Ru A2 caused a significant increase in yield by 0.5 kg/vine and 0.41 kg/vine, resp., compared to K 5BB. The annual mean values of the yield were between 0.58 kg/vine in the first harvest year in 1998 and 1.97 to 5.96 kg/vine in subsequent years. Bunch weight also showed a significant correlation with the rootstock. The average of all years showed that the bunch weight was positively influenced by K 125AA, G 26, 41 B and Binova. On the other hand the rootstocks G 9, 99 R, 101-14 MG and 3309 C caused a reduction of the bunch weight compared to K 5BB. The annual mean values ranged from a minimum of 149 grams in 1998 to a maximum of 318 g in 2007. The pruning wood weight was significantly affected by the rootstock, K 125AA, Binova, 225 Ru A2, 1103 P and Fercal causing increases between 0.014 kg/m2 and 0.03 kg/m2 compared to K 5BB; the rootstocks G 9, Riparia Portalis, 101-14 MG, 1616 C and Aripa, however, caused a reduction in pruning wood weight. The annual mean values were 0.03 kg/m2 and 0.094 kg/m2 in the first two years, and in subsequent years they were between 0.112 kg/m2 and 0.243 kg/m2. Microvinifications and tastings were carried out in a very limited extent with variants from selected rootstocks. In the sensory evaluation the rootstocks K 5BB, SO4 and T 5C, which are of importance in Austria, were rated the best.


Wurm L.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Gossinger M.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Wendelin S.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Korntheuer K.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg
Mitteilungen Klosterneuburg | Year: 2011

To test the yield performance and must quality of fourteen typical Austrian perry pear varieties (trained as spindle; planting system 4 x 2 m; rootstocks 'Pyrodwarf' (Pyrus communis) and 'Quitte A' (Cydonia oblonga) with interstem grafting), an experimental plot was planted in spring 2002 at the Haschhof experimental orchard of the Educational and Research Center for Viticulture and Pomology Klosterneuburg. Cultivation was conducted according to guidelines of organic production. 2006, in the fifth year after planting, the trees bore fruit for the first time. The highest cumulative yields were found with 'Dorschbirne'; 'Grüne Pichelbirne' and 'Speckbirne' also showed good yields. 'Machländer', 'Gelbe Wasserbirne', 'Grüne Winawitz', 'Rote Pichelbirne' and 'Schweizer Wasserbirne' were less fertile and showed a high tendency to biennial bearing. All varieties bore more fruit on the quince rootstock and grew significantly weaker than on the pear rootstock. Yield- or fruit quality-related attack by diseases and pests did not occur. The labour-intensive multiple picking by hand brought no significant improvement in must quality.


Gossinger M.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Eitner C.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Brandes W.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg | Vogl K.,Lehr und Forschungszentrum fur Wein und Obstbau Klosterneuburg
Mitteilungen Klosterneuburg | Year: 2011

The objective of this study was to calculate the influence of the low wine alcohol content (19 to 31 %vol.) and distillation speed (8 to 16 l/h) on the point of tails' separation (N-point) and the percentage rate of yield of head, heart and tail fractions with a concurrent distillation of apple mash. Furthermore some physical-chemical parameters (pH-value, conductivity, volatile acid) were tested in order to determine the N-point. For characterising the distillation process properly the average changes of the temperature of the lyne arm and the alcohol content of the distillation fractions were measured. Chemical analyses of the volatile substances in the fractions around the N-point by means of a gaschromatography were done as well. The results show that the alcohol content of the low wine as well as the distillation speed had a significant influence on the tails' separation point. The N-point was between 78 and 60 %vol. Due to the big ranges of the parameters pH-value, conductivity and volatile acid in the fractions around the N-point an exact measurement of the N-point by means of these parameters is not possible. The yield of heads, heart and tails depended significantly on the low wine alcohol content and ranged from 12 to 21 %, 18 to 30 % and 33 to 57 %, respectively. The lower the low wine alcohol content, the higher the percentage yield in the heart fractions. The average changes of the temperature of the lyne arm was between 0.04 and 0.32 °C per minute, those of the alcohol content between 0.08 and 0.80 %vol. per minute. No volatile substance (character impact compound) in the fractions around the N-point is qualified to determine the point of tails' separation.

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