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Patent
LaserGen | Date: 2016-08-08

Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.


Next Generation Sequencing Market is Expected to Reach US$ 7,527.0 Mn by 2021 SA-BRC is pleased to announce the release of its market research report on the "Global Endoscopy Devices Market Assessment & Forecast 2017-2021." Houston, TX, May 25, 2017 --( Illumina entered the NGS market through the acquisition of Solexa, Inc. in 2006. Since then, the company has introduced numerous variants of the sequencing-by-synthesis (SBS) technology. By far, Illumina has the largest installed base as compared to all other manufacturers worldwide. Illumina has developed strong distribution channels throughout the globe for effective distribution of its sequencing systems. Currently 54% of the company’s revenue is generated from the U.S., while 24% and 17% from Europe and Asia-Pacific regions respectively. In 2011, the company opened new office in Sao Paolo, Brazil to target the Latin America region where it has sparse market presence. In 2016, the company signed reseller agreement with GenDx; which allows the company to become a non-exclusive reseller of Illumina products in Benelux, Italy, United Kingdom, Germany and Finland. In 2015, Illumina announced its plans to invest in building a new European headquarters in Cambridge, U.K. This would strengthen its hold on the steadily growing Europe market. Request Free Report Sample@ http://www.sa-brc.com/Global-Next-Generation-Sequencing-Market-Assessment--Forecast-2017---2021/sample32 The report also maintains major focus on key player analysis in next generation sequencing market. Next generation sequencing (NGS) technology market is in a constant flux of change due to continuous technology upgrades. At least five companies are currently developing novel NGS technology which may commercialize during the forecast period. Numerous companies are investing in development of novel methods for efficient and cost effective sequencing. Agilent Technologies invested US$ 80 million in LaserGen for development and commercialization of the proprietary lightning terminators technology. Genapsys is currently developing Gene Electronic Nano-Integrated Ultra-Sensitive (GENIUS) technology, a portable sequencer targeted for research and diagnostic laboratory purposes. Although the company opened early access registration in 2014, commercial launch has not yet been announced officially. Although Nabsys had closed operations in 2015 after 10 years of struggle in development of its next generation sequencing technology, the company was relaunched as Nabsys 2.0 in 2016. The company also received US$ 42 million in funding from a Chinese company Zixin Pharma. Majority of the sequencing technologies in pipeline are nanopore based. Key players mentioned in the report include Illumina, Inc., Thermo Fisher Scientific, Inc., Oxford Nanopore, Pacific Biosciences, Qiagen N.V., Roche Sequencing, Stratos Genomics, GnuBio and others. More Report Description And TOC@ http://www.sa-brc.com/report/Global-Next-Generation-Sequencing-Market-Assessment--Forecast-2017---2021/32 Houston, TX, May 25, 2017 --( PR.com )-- According to the new report "Global Next Generation Sequencing Market Assessment & Forecast: 2017-2021" by SA-Business Research & Consulting Group, Illumina leads the market with more than 75% market share. The global next generation sequencing market was valued at US$ 3,509.6 Million in 2016 and is expected to reach US$ 7,527.0 Million by 2021 growing at a compounded annual growth rate of 16.02%.Illumina entered the NGS market through the acquisition of Solexa, Inc. in 2006. Since then, the company has introduced numerous variants of the sequencing-by-synthesis (SBS) technology. By far, Illumina has the largest installed base as compared to all other manufacturers worldwide. Illumina has developed strong distribution channels throughout the globe for effective distribution of its sequencing systems. Currently 54% of the company’s revenue is generated from the U.S., while 24% and 17% from Europe and Asia-Pacific regions respectively. In 2011, the company opened new office in Sao Paolo, Brazil to target the Latin America region where it has sparse market presence. In 2016, the company signed reseller agreement with GenDx; which allows the company to become a non-exclusive reseller of Illumina products in Benelux, Italy, United Kingdom, Germany and Finland. In 2015, Illumina announced its plans to invest in building a new European headquarters in Cambridge, U.K. This would strengthen its hold on the steadily growing Europe market.Request Free Report Sample@ http://www.sa-brc.com/Global-Next-Generation-Sequencing-Market-Assessment--Forecast-2017---2021/sample32The report also maintains major focus on key player analysis in next generation sequencing market. Next generation sequencing (NGS) technology market is in a constant flux of change due to continuous technology upgrades. At least five companies are currently developing novel NGS technology which may commercialize during the forecast period. Numerous companies are investing in development of novel methods for efficient and cost effective sequencing. Agilent Technologies invested US$ 80 million in LaserGen for development and commercialization of the proprietary lightning terminators technology. Genapsys is currently developing Gene Electronic Nano-Integrated Ultra-Sensitive (GENIUS) technology, a portable sequencer targeted for research and diagnostic laboratory purposes. Although the company opened early access registration in 2014, commercial launch has not yet been announced officially. Although Nabsys had closed operations in 2015 after 10 years of struggle in development of its next generation sequencing technology, the company was relaunched as Nabsys 2.0 in 2016. The company also received US$ 42 million in funding from a Chinese company Zixin Pharma. Majority of the sequencing technologies in pipeline are nanopore based.Key players mentioned in the report include Illumina, Inc., Thermo Fisher Scientific, Inc., Oxford Nanopore, Pacific Biosciences, Qiagen N.V., Roche Sequencing, Stratos Genomics, GnuBio and others.More Report Description And TOC@ http://www.sa-brc.com/report/Global-Next-Generation-Sequencing-Market-Assessment--Forecast-2017---2021/32 Click here to view the list of recent Press Releases from SA-BRC


Metzker M.L.,Baylor College of Medicine | Metzker M.L.,LaserGen
Nature Reviews Genetics | Year: 2010

Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest. © 2010 Macmillan Publishers Limited. All rights reserved.


The present invention relates generally to 3-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.


Grant
Agency: Department of Defense | Branch: Defense Health Program | Program: SBIR | Phase: Phase I | Award Amount: 150.00K | Year: 2012

Pathogen detection involving microbial forensics is an emerging field that presents enormous challenges for both the scientific and legal communities. Microbial pathogens of humans represent a highly diverse set of organisms known to cause disease and death. Microbes have also developed a number of elaborate mechanisms for generating natural genetic diversity. One major goal of microbial forensics is to use this genetic diversity to identify the source of a pathogen used to commit a crime or war offensive. Recent advances in next-generation sequencing (NGS) technologies have the potential to significantly alter the technological approaches used in characterizing field samples. This Phase I SBIR grant application proposes three aims: (i) identify the most efficient NGS platform by sequencing E. coli MG1655 using six platforms, (ii), conduct mixing experiments using purified gram negative and gram positive bacteria using the platform selected in aim (i), and (iii) conduct mixing experiments described in aim (ii) in the presence of human blood to simulate animal wound models. The outcome of the Phase I study lays the foundation for a Phase II proposal to develop method/system that would be portable and useful as a point-of-care device for pathogen identification in the field.


Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.


The present invention relates generally to 3-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.


The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.


The present invention relates generally to labeled and unlabeled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.


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