Lasbela University of Agriculture, Water and Marine Sciences

www.luawms.edu.pk
Uthal, Pakistan
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PubMed | University of Tübingen, Bryn Mawr College, Research Institute of Wildlife Ecology, Institute of Population Genetics and 14 more.
Type: Journal Article | Journal: Proceedings of the National Academy of Sciences of the United States of America | Year: 2016

Dromedaries have been fundamental to the development of human societies in arid landscapes and for long-distance trade across hostile hot terrains for 3,000 y. Today they continue to be an important livestock resource in marginal agro-ecological zones. However, the history of dromedary domestication and the influence of ancient trading networks on their genetic structure have remained elusive. We combined ancient DNA sequences of wild and early-domesticated dromedary samples from arid regions with nuclear microsatellite and mitochondrial genotype information from 1,083 extant animals collected across the species range. We observe little phylogeographic signal in the modern population, indicative of extensive gene flow and virtually affecting all regions except East Africa, where dromedary populations have remained relatively isolated. In agreement with archaeological findings, we identify wild dromedaries from the southeast Arabian Peninsula among the founders of the domestic dromedary gene pool. Approximate Bayesian computations further support the restocking from the wild hypothesis, with an initial domestication followed by introgression from individuals from wild, now-extinct populations. Compared with other livestock, which show a long history of gene flow with their wild ancestors, we find a high initial diversity relative to the native distribution of the wild ancestor on the Arabian Peninsula and to the brief coexistence of early-domesticated and wild individuals. This study also demonstrates the potential to retrieve ancient DNA sequences from osseous remains excavated in hot and dry desert environments.


PubMed | Shaheed Benazir Bhutto University, Baqai Medical University, Northwest University, China, Lasbela University of Agriculture, Water and Marine Sciences and University of Veterinary and Animal Sciences
Type: Journal Article | Journal: Veterinary world | Year: 2016

The aim of this study was to evaluate the impact of therapeutic and high doses of florfenicol on kidney and liver functional indicators in goat species.Six mature, healthy goats (combine breed and sex) with average weight 25 kg were selected for this study. The therapeutic (20 mg/kg b.w.) and high doses (40 and 60 mg) of florfenicol were administered for 3 days with 24 h interval. Blood samples were collected at 0, 24, 48, 72, 96, and 120 h following the each administered dose.The results showed that the therapeutic dose of florfenicol produced nonsignificant effect on serum urea, creatinine, total protein (TP), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT) and bilirubin on all timings, and increased (p<0.05) the serum glutamic oxaloacetic transaminase (SGOT) and serum glutamate-pyruvate transaminase (SGPT) levels for 48 h. Whereas the high doses of florfenicol (40 and 60 mg) significantly altered the kidney and liver functional indicators in the blood. In contrast with control, the serum urea level was (p<0.01) increased at all timing points. Creatinine values were altered (p<0.01, <0.05) in increasing manner from 24 to 96 h. The high dose of 40 mg decreased the TP (p<0.05) for 72 h and 60 mg persisted same effect (p<0.01) up to 120 h. The indices of ALP, GGT, SGOT, and SGPT were raised (p<0.01, <0.05) at all timings. The bilirubin indexes also (p<0.05) elevated from 48 to 72.It was concluded that the high doses of florfenicol produced reversible dose-dependent effects on functional indicators of kidney and liver such as urea, creatinine, TP, ALP, SGOT, SGPT, GGT, and bilirubin.


PubMed | Muhammad Nawaz Shareef Agriculture University, Lasbela University of Agriculture, Water and Marine Sciences, COMSATS Institute of Information Technology, Government College University at Faisalabad and Huazhong Agricultural University
Type: | Journal: Environmental science and pollution research international | Year: 2016

Knowledge of the dynamics of plant nitrogen (N) uptake at varying irrigation water levels is critical for strategizing increased N recovery efficiency (NRE), water use efficiency (WUE), and maize yield. The N dynamics were studied under various irrigation regimes to evaluate NRE, WUE, and maize yield. A pot experiment was conducted using three irrigation water regimes (50, 75, and 100% field capacity (FC)) and four N fertilizer rates (0, 1.6, 3.2, and 4.8g pot


Rehman H.U.,Lasbela University of Agriculture, Water and Marine Sciences | Aman A.,University of Karachi | Nawaz M.A.,University of Karachi | Qader S.A.U.,University of Karachi
Food Hydrocolloids | Year: 2015

A commercially available pectin degrading enzyme preparation is not usually pure and may contain several other auxiliary enzymes including pectin lyase and pectin methyl esterase with reasonably trace quantities of cellulases as well. Current work is focused to characterize an industrially important pectin degrading enzyme known as polygalacturonase produced by Bacillus licheniformis KIBGE-IB21 in term of its catalytic activity. The enzyme showed maximum activity when it was incubated for 05min at 45°C in an alkaline pH environment of pH-10.0. This enzyme is stable at a broad pH range and retained almost 100% of its initial activity in between pH 8.0 and 10.0 after 60min. It also exhibited high stability against different temperatures and 100% residual activity was measured at 30°C and 40°C up to 1h. The apparent Km and Vmax values for pectin degradation were 1.017mgml-1 and 23,800μMmin-1, respectively with an approximate molecular weight of 153kDa. Polygalacturonase also demonstrated exceptional storage stability at →20°C even after 30 days. © 2014 Elsevier Ltd.


Rajput I.R.,Zhejiang University | Rajput I.R.,Lasbela University of Agriculture, Water and Marine Sciences | Hussain A.,Zhejiang University | Li Y.L.,Zhejiang University | And 5 more authors.
Journal of Cellular Biochemistry | Year: 2014

Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that play a critical role to activate immune response. They may be targeted for immunomodulation by microbes, including probiotics. In this study, chicken bone marrow dendrite cells (chi-BMDCs) were stimulated with lipopolysachride (LPS), Saccharomyces boulardii (Sb), Bacillus subtilis B10 (Bs), co-culture of Sb + Bs and phosphate buffer saline (PBS) as a control group (Ctr) at 3, 6, and 12 h intervals. Results revealed that treatment groups modulated the phenotype and biological functions of chi-BMDCs. Scan electron microscopy showed attachment of probiotics on the surface of chi-BMDCs. Additionally transmission electron microscopy (TEM) revealed efficiently engulfing and degradation of probiotics. Gene expression levels of MHC-II, CD40, CD80 and CD86 up-regulated in stimulated groups. Furthermore, toll-like receptors TLR1, TLR2, TLR4, and chicken specific TLR15 expressions were improved and downstream associated factors MyD88, TRAF6, TAB1, and NFκ-B mRNA levels increased in all treatment groups as compared to control. Surprisingly, NFκ-B response was noted significant higher in LPS treatment among all groups. Moreover, IL-1β, IL-17, IL-4, TGF-β, and IL-10 production levels were found higher, and lower concentration of INF-γ and IL-8 were observed in Sb, Bs, and Sb + Bs treatment groups. In contrast, LPS groups showed prominent increase in IL-12, INF-γ, and IL-8 concentration levels as compared to control group. Altogether, these results emphasize a potentially important role of Saccharomyces boulardii and Bacillus subtilis B10 in modulating immunological functions of chi-BMDCs by targeting specific toll like receptors (TLRs) and associated factors. The role of probiotics on chi-BMDCs functionality in a non-mammalian species have been presented for the first time. © 2013 Wiley Periodicals, Inc. © 2013 Wiley Periodicals, Inc.


Bhutto B.,Sindh Agriculture University | Arijo A.,Sindh Agriculture University | Phullan M.S.,Lasbela University of Agriculture, Water and Marine Sciences | Rind R.,Sindh Agriculture University
International Journal of Agriculture and Biology | Year: 2012

Buffalo is considered as " black gold" in livestock herders in Pakistan, contributing 65% of the milk production and 26% of the total meat production of the country. Buffaloes suffer from a number of diseases, but a parasitic disease namely fascioliasis is more common and causing huge economic losses. A survey was carried out to determine the prevalence of fascioliasis in buffaloes of different agro-climatic conditions of Sindh province of Pakistan. A total of 1800 fecal samples were randomly collected from buffaloes of different sex and age groups. The overall prevalence of fascioliasis was 42.06%; and all positive samples were infected with Fasciola gigantica. District-wise prevalence of fascioliasis in buffaloes was recorded as 41.83, 30.83 and 53.50%, in Larkana, Hyderabad and Badin districts, respectively selected from three zones of Sindh province namely Northern, Mid and Southern Sindh, respectively. Month-wise prevalence reflected that the higher rate in colder months viz. December (58.67%) and January (61.33%), while the lowest in the warm months: May (31.33%), June (24.67%) and July (26.00%). Sex-wise data revealed more than double incidence in female (45.08%) in comparison of male (20.89%). The highest level of infection was found in older group i.e., above 6 years (62.62%) followed by in age groups of 4-6 years (57.28%), 2-4 years (42.56%) and up to 2 year (17.87%). Significant correlation was recorded in between humidity and prevalence of fascioliasis in buffaloes; however no any significant relationship could be recorded between rainfall and infection. © 2012 Friends Science Publishers.


Waqar-Ul-Haq,Lasbela University of Agriculture, Water and Marine Sciences | Munir M.,Pmas Arid Agriculture University | Akram Z.,Pmas Arid Agriculture University
Pakistan Journal of Botany | Year: 2010

The experimental material was sown in the experimental area of Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi during 2004-2005, comprising of 10 wheat genotypes viz., Chakwal-86, Iqbal-2000, Uqab-2000, GA-2002, 00FJ03, IC- 001, IC-002, NR-234, 3C061 and 3C062 for determination of interrelationships among yield and yield related characters. Analysis of variance showed highly significant differences among the genotypes for all the traits. The correlation coefficient indicated that spike length, number of spikes per plant, number of spikelets per spike, number of grains per spike, number of tillers per m2, 1000grain weight were significantly and positively correlated with grain yield per plant, while days to heading, days to maturity and plant height showed non significant correlation with grain yield per plant.


PubMed | Lasbela University of Agriculture, Water and Marine Sciences
Type: Journal Article | Journal: Asian Pacific journal of cancer prevention : APJCP | Year: 2016

Breast cancer is very common and the leading cause of cancer deaths among women globally. Hereditary cases account for 510% of the total burden and CHEK2, which plays crucial role in response to DNA damage to promote cell cycle arrest and repair or induce apoptosis, is considered as a moderate penetrance breast cancer risk gene. Our objective in the current study was to analyze mutations in related to breast cancer. A total of 271 individuals including breast cancer patients and normal subjects were enrolled and all 14 exons of CHEK2 were amplified and sequenced. The majority of the patients (>95%) were affected with invasive ductal carcinoma (IDC), 52.1% were diagnosed with grade III tumors and 56.2% and 27.5% with advanced stages III and IV. Two novel nonsense variants i.e. c.58C>T (P.Q20X) and c.256G>T (p.E85X) at exon 1 and 2 in two breast cancer patients were identified, both novel and not reported elsewhere.


PubMed | Lasbela University of Agriculture, Water and Marine Sciences and Huazhong University of Science and Technology
Type: Journal Article | Journal: Nanoscale | Year: 2016

Herein, we design novel cellulose conjugated mesoporous silica nanoparticle (CLS-MSP) based nanotherapeutics for stimuli responsive intracellular doxorubicin (DOX) delivery. DOX molecules are entrapped in pores of the fabricated mesoporous silica nanoparticles (MSPs) while cellulose is used as an encapsulating material through esterification on the outlet of the pores of the MSPs to avoid premature DOX release under physiological conditions. In in vitro studies, stimuli responsive DOX release is successfully achieved from DOX loaded cellulose conjugated mesoporous silica nanoparticles (DOX/CLS-MSPs) by pH and cellulase triggers. Intracellular accumulation of DOX/CLS-MSPs in human liver cancer cells (HepG2 cells) is investigated through confocal microscope magnification. Cell viability of HepG2 cells is determined as the percentage of the cells incubated with DOX/CLS-MSPs compared with that of non-incubated cells through an MTT assay.


PubMed | Lasbela University of Agriculture, Water and Marine Sciences
Type: Journal Article | Journal: Asian Pacific journal of cancer prevention : APJCP | Year: 2016

Breast cancer is the most commonly occurring and leading cause of cancer deaths among women globally. Hereditary cases account 5-10% of all the cases and CHEK2 is considered as a moderate penetrance breast cancer risk gene. CHEK2 plays a crucial role in response to DNA damage to promote cell cycle arrest and repair DNA damage or induce apoptosis. Our objective in the current study was to analyze mutations in the CHEK2 gene related to breast cancer in Balochistan. A total of 271 individuals including breast cancer patients and normal subjects were enrolled. All 14 exons of CHEK2 were amplified and sequenced. The majority of the patients (>95%) had invasive ductal carcinomas (IDCs), 52.1% were diagnosed with tumor grade III and 56.1% and 27.5% were diagnosed with advance stages III and IV. Two novel nonsense variants i.e. c.58C>T (P.Q20X) and c.256G>T (p.E85X) at exon 1 and 2 in two breast cancer patients were identified in the current study. Both the variants identified were novel and have not been reported elsewhere.

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