Entity

Time filter

Source Type

Prague, Czech Republic

Jelinek M.,ASCR Institute of Physics Prague | Jelinek M.,Czech Technical University | Weiserova M.,Academy of Sciences of the Czech Republic | Kocourek T.,ASCR Institute of Physics Prague | And 4 more authors.
Laser Physics | Year: 2011

Thin films of hydroxyapatite (HA) and silver-doped HA were synthesized using KrF excimer laser deposition. Material was ablated from one target composed from silver and HA segments. Layers properties as silver content, structure, color, FTIR spectra and antibacterial properties (Gram-positive Bacillus subtilis) were measured. Silver concentration in HA layers of 0.06, 0.3, 1.2, 4.4, 8.3, and 13.7 at % was detected. The antibacterial efficacy changed with silver dopation from 71.0 to 99.9%. The focus is on investigation of minimum Ag concentration needed to reach a high antibacterial efficacy. © 2011 Pleiades Publishing, Ltd. Source


Jelinek M.,ASCR Institute of Physics Prague | Jelinek M.,Czech Technical University | Kocourek T.,ASCR Institute of Physics Prague | Kocourek T.,Czech Technical University | And 7 more authors.
Applied Physics A: Materials Science and Processing | Year: 2010

Thin hydroxyapatite (HA), silver-doped HA and silver layers were prepared using a pulsed laser deposition method. Doped layers were ablated from silver/HA targets. Amorphous and crystalline films of silver concentrations of 0.06 at.%, 1.2 at.%, 4.4 at.%, 8.3 at.% and 13.7 at.% were synthesized. Topology was studied using scanning electron microscopy and atomic force microscopy. Contact angle and zeta potential measurements were conducted to determine the wettability, surface free energy and electric surface properties. In vivo measurement (using Escherichia coli cells) of antibacterial properties of the HA, silver-doped HA and silver layers was carried out. The best antibacterial results were achieved for silver-doped HA layers of silver concentration higher than 1.2 at.%. © 2010 Springer-Verlag. Source


Jelinek M.,ASCR Institute of Physics Prague | Jelinek M.,Czech Technical University | Kocourek T.,ASCR Institute of Physics Prague | Kocourek T.,Czech Technical University | And 9 more authors.
Materials Science and Engineering C | Year: 2013

Hydroxyapatite layers with silver doping from 0.06 at.% to 14 at.% were prepared by laser deposition. The films' physical properties such as morphology, composition, crystallinity, Young's modulus and microhardness were measured. Films were amorphous or polycrystalline in dependence on deposition temperature (from RT to 600 C). Antibacterial properties were tested using Escherichia coli and Bacillus subtilis cells. The antibacterial efficacy changed with silver doping from 4% to 100%. Cytotoxicity was studied by a direct contact test. Depending on doping and crystallinity the films were either non-toxic or mildly toxic. © 2012 Elsevier B.V. All Rights Reserved. Source


Jelinek M.,ASCR Institute of Physics Prague | Jelinek M.,Czech Technical University | Weiserova M.,Academy of Sciences of the Czech Republic | Kocourek T.,ASCR Institute of Physics Prague | And 3 more authors.
Laser Physics | Year: 2010

The contribution deals with KrF laser synthesis and study of doped biocompatible materials with focus on diamond-like carbon (DLC) and hydroxyapatite (HA). Overview of materials used for dopation is given. Experimental results of study of HA layers doped with silver are presented. Films properties were characterized using profilometer, SEM, WDX, XRD and optical transmission. Content of silver in layers moved from 0.06 to 13.7 at %. The antibacterial properties of HA, silver and doped HA layers were studied in vivo using Escherichia coli cells. © 2010 Pleiades Publishing, Ltd. Source


Strnadova M.,Lasak Ltd. | Strnad Z.,Lasak Ltd. | Sponer P.,Charles University | Jirosova J.,Lasak Ltd. | Strnad J.,Lasak Ltd.
Key Engineering Materials | Year: 2012

The aim of this study was to evaluate the osteoconductive properties of synthetic porous hydroxyapatite prepared by low-temperature microwave processing OssaBase® HA (SPHA) in comparison with biological apatite, non-sintered deproteinized bovine bone Bio-Oss® (DBB). The materials were implanted into the bone sockets of the tibia of Beagle dogs for 3 and 6 months. The bone response to granules of the materials of similar sizes was compared. Histological analysis of the specimens with histomorphometry was performed at different times after in vivo implantation. Based on the histological analysis, the level of bone formation in the spaces between the implanted granules and through the interconnected pores of both implanted materials within a cortical region was significantly higher (bone area ingrowth 72-85%) than within a cancellous bone site (bone area ingrowth 16-28%) at three and six months after implantation. According to our study, the bioactive and osteoconductive properties (bone implant contact and bone area ingrown) of the synthetic porous hydroxyapatite are very high and comparable with the biological apatite, non-sintered deproteinized bovine bone. The favourable influence of the high specific surface area and carbonate content of the synthetic, porous hydroxyapatite on bone formation was confirmed. © (2012) Trans Tech Publications. Source

Discover hidden collaborations