Las Tunas, Cuba

Las Tunas University

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Las Tunas, Cuba
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Perez Guzman R.E.,Las Tunas University | Noriega Aleman M.,University of the East of Cuba | Cespedes Perez A.,Las Tunas University
Revista Cubana de Investigaciones Biomedicas | Year: 2016

Introduction: Cardiovascular diseases represent the most frequent cause of premature death and disability worldwide. They are the second cause of death in Cuba, with sizeable increase in a short period of time. Objectives: To make a comparative study of the main automatic delineators of electrocardiographic signals based on the Transformed Wavelet in order to confirm the effectiveness of each of them at the time of detecting and delineating the start of the QRS complex, the main peak of the wave in QRS complex and the end of the T-wave. Methods: The study was performed by using the delineation of a set of simulated signals affected by the mechanical effect of respiration and noise taken from real registers of stress tests. Additionally, the different uniderivational and multiderivational automatic systems of electrocardiographic signal delineation based on Wavelet Transform and they were applied to the referred signals. Results: The variability of the uniderivational method errors for the different leads was confirmed; hence it is not easy to choose one of the 12 leads as the most suitable for the wave delineation. The multiderivational strategies perform better in the wave peak and in the waves with lower signal-to-noise ratio. Conclusions: The delineation methods based on Wavelet Transform do not require any prefiltering or preprocessing for noise elimination. The multiderivational method is the one that makes the best use of the spatial information provided by the orthogonal leads, thus allowing a more precise delineation of the electrocardiographic signal in the waves with lower signal/noise ratio. © 2016, Editorial Ciencias Medicas. All rights reserved.


Acosta K.,Las Tunas University | Silva F.N.,Federal University of Viçosa | Silva F.N.,Santa Catarina State University | Zamora L.,National Center for Animal and Plant Health | And 6 more authors.
Journal of Plant Pathology | Year: 2015

An emerging soybean disease observed in Cuba was investigated in view of the possibility that a phytoplasma could be involved in its aetiology. Thirty-five soybean plants showing symptoms of stunting, chlorosis, crinkle and aborted seed pods were collected in Las Tunas and Holguin provinces during 2013, and analysed by nested- PCR with primers targeting the 16S ribosomal DNA (16S rDNA). Phytoplasmas were detected in 83% of symptomatic soybean plants. Conventional and virtual RFLP analyses of 16S rDNA sequences revealed the presence of strains of ‘Candidatus Phytoplasma asteris’ (16SrI group). Phytoplasmas belonging to ribosomal subgroup 16SrI-B and two putative new ribosomal subgroups 16SrI-W and 16SrI-Y were identified. Phylogenetic analysis corroborated the RFLP analyses, in which the Cuban strains formed a clade with representative sequences of the 16SrI group. 16SrI-B was the most prevalent subgroup (45% of positive samples) and mixed infections of different subgroups were observed (10% of the positive samples). The partial 16S rDNA sequences of three strains showed 99% nucleotide identity to several GenBank sequences of group 16SrI phytoplasmas, including a phytoplasma associated with a soybean disease in Brazil. This is the first report of a phytoplasma belonging to the 16SrI subgroup occurring in soybean in Cuba. © 2015, Edizioni ETS. All rights reserved.


Acosta K.,Las Tunas University | Zamora L.,National Center for Animal and Plant Health | Pinol B.,National Center for Animal and Plant Health | Fernandez A.,Las Tunas University | And 6 more authors.
Crop Protection | Year: 2013

Two different papaya diseases have been previously reported in Cuba, Bunchy Top Symptom (BTS) associated with a phytoplasma of group 16SrII '. Candidatus Phytoplasma aurantifolia' and Papaya Bunchy Top (PBT), associated with a rickettsia. Regarding the regional phytosanitary impact of both diseases for the papaya crop, the present study investigated the occurrence of BTS and PBT in papaya fields in Cuba, and the possible mixed infection of phytoplasma and rickettsia pathogens associated. Papaya plants showing symptoms of BTS or PBT or both, were collected in Las Tunas and Havana provinces from January 2009 to February 2010, and evaluated for phytoplasma and rickettsia by PCR with primers targeting the 16S ribosomal RNA and the rickettsial succinate deshydrogenase (sdhA) genes, respectively. Phytoplasmas and rickettsia were individually detected in 76/86 BTS-symptomatic and 22/22 PBT-symptomatic papaya plants, and simultaneously detected in 5/86 (5.81%) of the BTS-symptomatic and 17/22 (77.27%) of the PBT-symptomatic plants. Conventional and virtual RFLP analyses of the 16S rDNA sequences revealed the presence of phytoplasmas of group 16SrI '. Candidatus Phytoplasma asteris' and 16SrII in papaya plants affected by BTS and PBT, and identified two new phytoplasma subgroups, 16SrI-X and 16SrII-N in papayas fields of Las Tunas, which was confirmed by phylogenetic analysis. The partial rickettsia sdhA gene sequences were 100% identical to that of the rickettsia associated with PBT in Puerto Rico. Results confirm that phytoplasmas are consistently associated with both BTS and PBT symptoms, and that mixed infections of phytoplasma and rickettsia pathogens can occur in either BTS or PBT-affected papaya fields, which implies new epidemiological constraints for the disease control. © 2012 Elsevier Ltd.


Perez K.A.,Las Tunas University | Pinol B.,National Center for Animal and Plant Health | Rosete Y.A.,Rothamsted Research | Rosete Y.A.,CABI Inc | And 3 more authors.
Journal of Phytopathology | Year: 2010

Transmission tests were conducted with field-collected Bunchy Top Symptoms (BTS) phytoplasma-infected specimens of Empoasca papayae. BTS developed in all eight inoculated papayas 3 months later. The BTS phytoplasma was identified in six of eight inoculated papayas, whose partial 16S rRNA sequence (GenBank Accession no. FJ6492000) was 99.9% identical with those from the collected papayas (GenBank Accession no FJ649198) and E. papayae (GenBank Accession no. FJ649199), all of which are members of group 16SrII, '. Candidatus Phytoplasma aurantifolia'. Results confirmed the ability of E. papayae to transmit the BTS phytoplasma. © 2009 Blackwell Verlag GmbH.


Estevez M.,National Institute of Neurology and Neurosurgery | Machado C.,National Institute of Neurology and Neurosurgery | Leisman G.,National Institute of Brain and Rehabilitation science | Leisman G.,University of Habana | And 4 more authors.
International Journal on Disability and Human Development | Year: 2016

Spectral analysis (SA) has been extensively applied to the assessment of heart rate variability. Traditional methods require the transformation of the original non-uniformly spaced electrocardiogram RR interval series into regularly spaced ones using interpolation or other approaches. The Lomb-Scargle (L-S) method uses the raw original RR series, avoiding different artifacts introduced by traditional SA methods, but it has been scarcely used in clinical settings. An RR series was recorded from 120 healthy participants (17-25 years) of both genders during a resting condition using four SA methods, including the Classic modified periodogram, the Welch procedure, the autoregressive Burg method and the L-S method. The efficient implementation of the L-S algorithm with the added possibility of the application of a set of options for the RR series pre-processing developed by Eleuteri et al., and also the results obtained in this study, show that the L-S method can be a good choice for future clinical studies. The L-S method seems particularly useful when the heart rates of studied participants will be below 60 or over 120 beats per minute. Nevertheless, it is important to the development of a smoothing procedure for the L-S spectra to avoid the picky behavior of the L-S power spectrum. The implementation of the L-S algorithm used in this study has been recently published by other authors included in our references, and brings some particular filtering features. The results obtained, comparing the four spectral methods, show that this implementation seems particularly useful when the heart rates of studied participants will be below 60 or over 120 beats per minute. Nevertheless, it is important to recommend for all existing L-S software implementations, the development of a smoothing procedure to avoid the picky behavior of the L-S power spectrum. © 2016 by De Gruyter.


Galindo J.,Institute Ciencia Animal | Gutierrez O.,Institute Ciencia Animal | Ramayo M.,Empresa Agropecuaria MINAZ | Leyva L.,Las Tunas University
Cuban Journal of Agricultural Science | Year: 2014

The mineral status of cows and its relationship with the soil-pasture system in a dairy unit of the Eastern region of Cuba was studied. For soil analysis, Na, K, Mg, Ca and P contents were determined in the different animal feed production areas. The chemical composition of pastures was established and blood samples were taken to 10 % of the herd for analyzing their macro and microelement composition. The mineral content of soils varied regarding the agroproductive system where animals grazed. Pastures presented CP contents lower than those reported for the species. Cows showed severe Cu and Zn deficiencies, below the contents considered as normal. The Ca/P relationship was not adequate. It is concluded that the productive systems under study show mineral deficiencies. The use of correction mineral formulas is recommended.


Objective: Determine the usefulness of immunoinformatics tools to detect potentially immunodominant peptides, and evaluate the differences between the immune responses provided by the animal models used in preclinical and human studies. Methods: Modeling was conducted of the response to two exogenous proteins: recombinant streptokinase and hepatitis B surface antigen. Based on their primary sequences, algorithms were used to identify B and T epitopes against HLA class I and II molecules (HLA-A*0201, HLA-DRB1*0301 and HLA-DRB1*0701), and murine haplotypes H2-Kd and H2-Kk. The highest scoring peptides were chosen. Results: ABCPred algorithm showed a better prediction capacity for B epitopes, whereas coincidence was greater in modeling programs for the T response. The epitopes generated for haplotype H2-Kk had greater similitude with those presented by the HLA molecules selected. Conclusions: A methodology is presented which is applicable to the development of subunit and multiepitope vaccines, as well as other peptidic biotechnological drugs. This methodology allows optimization of the preclinical and clinical phases at a very low cost, with minimal technological requirements, optimal use of media, and resources and human capital available at any institution of the national health system.


For directive and nutritionists in food factories balanced for the animal consumption is of interest the minimization of the use level in formula of ingredients of nutritious high value and cost of acquisition and that the balance of nutritious in formula remains in permissible ranges. The objective is generally to diminish the consumption of Soya, expensive and present ingredient in most of the mixtures with level of use average superior to 18% for Egg-laying Hens. The investigation is developed with the objective of determining nutritional and economic differences among formulas where the use of an ingredient is minimized accurately in the coefficients of the function objective and precision and imprecisión in the nutritious Gross Total Protein of ingredients regarding formulas, at minimum cost, with precise data in the objective and precise and imprecise in the nutritional contribution of ingredients. For such purposes mathematical models of Lineal Programming and Lineal Programming Fuzzy of the Diet Problem was built. The phenomenon of the imprecisión was introduced starting from the inherent variability of the Gross Total Protein of the ingredients in formula, being represented through Numbers Fuzzy associated to the nutrient in question. The solution of the different models allowed to obtain feasible solutions with the established satisfied nutritional requirements. From the economic point of view increase takes place in the cost, being concluded that the biggest increments in the cost happen when you don't take in consideration the variability of the Gross Total Protein of the ingredients.


Historically, in Cuba, the tendency exists of carrying out the biggest productions of blended foods balanced for the Poultry keeping and the biggest expenses caused by this concept they take place in productions dedicated to Egg-laying Hens. With the objective of determining relationships that link the cost of production of the egg, in Egg-laying Hens, with indicators related with the zootechny and nutrition was carried out a study about the cost in productions of the egg associated to these animals. In the period of one year, for different farms and regions of the country, Multiple Regression was used to obtain statistical important models that involve an important number of observed variables related with the nutrients of the offered portion and the cost of the egg. Inside the variables it also figures the ambient temperature and you were determined statistically that this enters in direct relationship with the percentage of setting of the hens, all that which are of importance and interest for the personnel that are in charge of of the handling of the animals. Finally you concludes that the cost of the egg can be calculated starting from zootechny elements and nutrition, what indicate the importance of the formula of the portion and being inferred that a reduction of your cost, maintaining the nutritional parameters and required quality, you will produce a decrease in the cost of the animal associated production. You also concludes that the cost of the egg depends on the setting percentage and this of the ambient temperature, for what are important to have this element in consideration for the corresponding analyses of productivity. The final conclusions of the work are: - the cost of the egg can be calculated starting from zootechny and nutrition elements, what indicate the importance of the formula of the portion and being inferred that a reduction of your cost, maintaining the nutritional parameters and required quality, you will produce a decrease in the cost of the associated animal production. - the cost of the egg depends on the setting percentage and this of the ambient temperature, for what are important to have this element in consideration for the corresponding analyses of productivity.

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