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Li J.-Q.,Chinese Academy of Agricultural Sciences | Li J.-Q.,Lanzou Institute of Biological Products | Cheng J.,Chinese Academy of Agricultural Sciences | Lan X.,Chinese Academy of Agricultural Sciences | And 8 more authors.
Virologica Sinica | Year: 2010

The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5′ non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS. © 2010 Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg.

Li J.-Q.,Lanzou Institute of Biological Products | Yang J.-J.,Lanzou Institute of Biological Products | Fan X.-J.,Lanzou Institute of Biological Products | Sun Z.-P.,Lanzou Institute of Biological Products | And 4 more authors.
Virologica Sinica | Year: 2012

Enterovirus 71 (EV71) is a member of the Entero-virus genus of the Picornaviridae family and is the major cause of Hand, foot, and mouth disease (HFMD) in children. Different strains from Gansu were cloned and the P1 protein was sequenced and analysed. Results indicate that there are three kinds of EV71 infections prevalent in Gansu. The VP1 protein from one of these strains, 55F, was expressed. The recombinant protein was expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and after the fourth injection a high titer of antiserum was detected by ELISA assay. These data are useful for further clarification of prevalent EV71 strains in the north of China at the molecular level and provide a basis for EV71 diagnosis. © Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2012.

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