Stahnke H.,German Federal Institute for Risk Assessment |
Kittlaus S.,Carl Zeiss GmbH |
Kempe G.,Landesuntersuchungsanstalt fur das Gesundheits und Veterinarwesen Sachsen |
Hemmerling C.,Landeslabor Berlin Brandenburg |
Alder L.,German Federal Institute for Risk Assessment
Journal of Mass Spectrometry | Year: 2012
This study investigates to which extent the design of electrospray ion sources influences the susceptibility to matrix effects (MEs) in liquid chromatography-tandem mass spectrometry (LC-MS/MS). For this purpose, MEs were measured under comparable conditions (identical sample extracts, identical LC column, same chromatographic method and always positive ion mode) on four LC-MS/MS instrument platforms. The instruments were combined with five electrospray ion sources, viz. Turbo Ion Spray, Turbo VTM Source, Standard ESI, Jet Stream ESI and Standard Z-Spray Source. The comparison of MEs could be made at all retention times because the method of permanent postcolumn infusion was applied. The MEs ascertained for 45 pesticides showed for each electrospray ion source the same pattern, i.e. the same number of characteristic signal suppressions at equivalent retention times in the chromatogram. The Turbo Ion Spray (off-axis geometry), Turbo VTM Source (orthogonal geometry) and the Standard Z-Spray Source (double orthogonal geometry) did not differ much in their susceptibility to MEs. The Jet Stream ESI (orthogonal geometry) reaches a higher sensitivity by an additional heated sheath gas, but suffers at the same time from significantly stronger signal suppressions than the comparable Standard ESI (orthogonal geometry) without sheath gas. No relation between source geometry and extent of signal suppression was found in this study. Copyright © 2012 John Wiley & Sons, Ltd.
Forster A.,German Research Center for Geosciences |
Schoner R.,Friedrich - Schiller University of Jena |
Forster H.J.,German Research Center for Geosciences |
Norden B.,German Research Center for Geosciences |
And 5 more authors.
Marine and Petroleum Geology | Year: 2010
Ketzin, in the Northeast German Basin (NEGB), is the site for pilot injection of CO2 (CO2SINK project) into a saline aquifer (the Upper Triassic Stuttgart Formation) situated at a depth of about 630-700m. This paper reports the baseline characterization of the reservoir formation based on new core material and well-logs obtained from one injection well and two observations wells, drilled at a distance from 50m to 100m from each other. The reservoir is lithologically heterogeneous and made up by fluvial sandstones and siltstones interbedded with mudstones showing remarkable differences in porosity. The thickest sandstone units are associated with channel sandstone, whose thickness varies over short lateral distances. In-depth petrographic, mineralogical, mineral-chemical, and whole-rock geochemical analysis were performed focusing on the sandstone intervals, which display the best reservoir properties for CO2 injection. The dominantly fine-grained and well to moderately-well sorted, immature sandstones classify as feldspathic litharenites and lithic arkoses. Quartz (22-43wt.%), plagioclase (19-32wt.%), and K-feldspar (5-13wt.%) predominate mineralogically. Muscovite plus illite and mixed-layer minerals are omnipresent (4-13wt.%). Quartz, feldspar, as well as meta-sedimentary and volcanic rock fragments comprise the most abundant detrital components, which often are rimmed by thin, early diagenetic coatings of ferric oxides, and locally of clay minerals. Feldspar grains may be unaltered and optically clear, partially to completely dissolved, partially altered to sheet silicates (mainly illite), or albitized. Analcime and anhydrite constitute the most widespread, often spatially associated pore-filling cement minerals. Authigenic dolomite, barite, and coelestine is minor. The percentage of cements ranges in total from about 5vol.% to 32vol.%. Except of samples intensely cemented by anhydrite and analcime, total porosities of the sandstones range from 13% to 26%. The fraction of intergranular porosity varies between 12% and 21%. About 1-5% porosity has been generated by dissolution of detrital plagioclase, K-feldspar, and volcanic rock fragments. The comparatively large modal abundance of feldspars, micas, chlorite, clay minerals, Fe-Ti-oxides, and analcime account for the richness in Ti, Al, Fe, Mg, Na, and K, and the paucity in Si, of the Stuttgart sandstones relative to mature sandstones. Altogether, these sandstones are comparatively rich in minerals that may potentially react with the injected CO2. © 2010 Elsevier Ltd.
Geiss S.,Thuringer Landesanstalt fur Umwelt und Geologie |
Loffler D.,Bundesanstalt fur Gewasserkunde |
Korner B.,Karlsruhe Institute of Technology |
Engelke M.,Landeslabor Berlin Brandenburg |
And 2 more authors.
Microchemical Journal | Year: 2015
A method for the quantification of the sum of short chain chlorinated paraffins (SCCPs) by GC-ECNI-MS was developed. This method is suited to the special demands of environmental sediment analysis using an approach of sum determination by multiple regression quantification and is independent of the chlorine content as well as robust against changes in C-number distribution of the SCCPs to be analysed. The method is based on the integration of the signals for two selected m/z values (375 and 423) over the full retention range of the SCCPs. The calibration is performed using three synthetic mixtures representing the variety of SCCP-mixtures as found in environmental samples as well as in technical mixtures. Quantification is performed using a multiple linear regression calibration with two selected m/z value signals achieving an LOQ of 0.6. μg/g sediment.The proposed quantification procedure for sediments was verified by 16 independently prepared and differently composed SCCP-mixtures mimicking a variety of sediment samples quoted in literature.For the analysis of the sediment samples a two-step clean-up was applied comprising a column chromatographic clean-up and GPC.The method allows for a standardized analysis according to the European water framework directive, especially in the case of trend monitoring of sediments. © 2014 Elsevier B.V.
Eisenberg T.,Landesbetrieb Hessisches Landeslabor |
Kutzer P.,Landeslabor Berlin Brandenburg |
Peters M.,Chemisches und Veterinaruntersuchungsamt Westfalen |
Sing A.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit |
And 2 more authors.
Emerging Infectious Diseases | Year: 2014
Corynebacterium ulcerans may cause diphtheria in humans and caseous lymphadenitis in animals. We isolated nontoxigenic tox-bearing C. ulcerans from 13 game animals in Germany. Our results indicate a role for game animals as reservoirs for zoonotic C. ulcerans.
Pannwitz G.,Veterinaramt Landkreis Ostvorpommern |
Freuling C.,Institute for Epidemiology |
Denzin N.,Landesamt fur Verbraucherschutz des Landes Sachsen Anhalt |
Schaarschmidt U.,TU Chemnitz |
And 11 more authors.
Epidemiology and Infection | Year: 2012
Between 1985 and 2008, a total of 102 387 wild boar sera originating from Eastern Germany covering an area of 108 589 km2 were tested for the presence of Aujeszky's disease virus (ADV)-specific antibodies. From 1985 until 1991 and from 1992 until 2008, wild boar sera were exclusively investigated using either conventional seroneutralization assays (n=39 621) or commercial gB and full antigen ELISAs (n=62 766), respectively. Spatial-temporal analysis revealed an increasing ADV seroprevalence from 0.4% to 15.9%, on average, during the 24-year observation period that went along with a continuous spread of the infection in a western direction. During 2006 and 2008, 18% of the 66 affected districts had ADV seroprevalences >30%. There was a significant correlation between ADV seroprevalence and the hunting index of population density (HIPD) of wild boar in the entire study area, although this did not hold true for some regions. Seroprevalences did not differ between sexes but were age-dependent. East Germany has been officially free of Aujeszky's disease (pseudorabies) in domestic pigs since 1985. Although a risk for domestic pigs cannot be completely ruled out, experience has shown that ADV in domestic pigs could be eliminated although the virus was present in the wild boar population. Despite increasing ADV seroprevalence in the East German wild boar population no spillover infections from wild boar to domestic pigs have been reported. To further trace ADV infections in the wild boar population in Germany, a nationwide serological monitoring programme should be implemented. © 2011 Cambridge University Press.
Kuehn A.,Robert Koch Institute |
Kuehn A.,Helmholtz Center Munich |
Schulze C.,Landeslabor Berlin Brandenburg |
Kutzer P.,Landeslabor Berlin Brandenburg |
And 4 more authors.
Epidemiology and Infection | Year: 2013
SUMMARY A total of 2475 animals from Germany, both captive and wild, were tested for antibodies against Francisella tularensis to obtain more knowledge about the presence of this pathogen in Germany. An indirect and a competitive ELISA served as screening methods, positive and inconclusive samples were confirmed by Western blot. Of the zoo animals sampled between 1992 and 2007 (nÂ =Â 1122), three (0.3%) were seropositive. The seroconversion of a hippopotamus in Berlin Zoo was documented. From 1353 serum samples of wild foxes (Vulpes vulpes), raccoon dogs (Nyctereutes procyonoides) and wild boars (Sus scrofa), collected between 2005 and 2009 in the federal state of Brandenburg (surrounding Berlin), a total of 101 (7.5%) tested positive for antibodies to F. tularensis lipopolysaccharide. Our results indicate a higher seroprevalence of F. tularensis in wildlife in eastern Germany than commonly assumed. Furthermore, we found foxes and raccoon dogs to be biological indicators for tularaemia. Copyright © Cambridge University Press 2012.
PubMed | LABOKLIN GmbH & Co. KG, Free University of Berlin, Bayer Vital GmbH, Landeslabor Berlin Brandenburg and Bayer Animal Health GmbH
Type: | Journal: Veterinary parasitology | Year: 2016
Dirofilaria repens is endemic in eastern and southern European regions but was recently found in Germany in dogs, mosquitoes and one human patient. Since some of the positive dog and mosquito samples were collected in Brandenburg, it was aimed to systematically assess the prevalence of D. repens and other canine vector-borne pathogens in Brandenburg. Dog owners also received a questionnaire and were asked to provide more information about the dogs including travel history. In total, 1023 dog blood samples as well as 195 fox spleen and 179 fox blood samples were collected. DNA was analysed by PCR for the presence of filariae, piroplasms, anaplasmataceae and Rickettsia spp. Filariae were detected in six dogs (0.6%), two were positive for DNA from D. repens, two from Dirofilaria immitis and two from Acanthocheilonema reconditum. One of the D. repens positive dogs originated from an animal shelter in Brandenburg, but the origin of the other one remained unknown. Interestingly, both D. repens ITS-1 sequences showed 100% identity to a D. repens sample obtained from a Japanese woman that travelled in Europe and were 97% identical to a newly proposed species Dirofilaria sp. hongkongensis described from Hong Kong. However, identity to other D. repens sequences from Thailand was considerably lower (81%). Identity of 12S rRNA and cytochrome oxidase I to D. repens samples from southern Europe was 99%. Due to the low number of Dirofilaria spp. positive dogs and since the origin of these was unknown, endemic occurrence of Dirofilaria in Brandenburg could not be confirmed. Anaplasma phagocytophilum was found in 15 dogs (1.5%), Candidatus Neoehrlichia mikurensis in three dogs (0.3%) and E. canis in one dog (0.1%), which was co-infected with D. repens. Rickettsia spp. were detected in 8 dogs (0.8%), seven were Rickettsia raoultii and one was Rickettsia felis. To the authors knowledge, R. raoultii DNA was detected for the first time in dogs in Germany in this study and Candidatus N. mikurensis for the second time. In spleen samples of red foxes with 47.5% a high prevalence of piroplasms was found. Sequencing of 11 samples identified 10 as Theileria annae. Despite the high prevalence of this pathogen in its reservoir host, it was absent in dog samples. In one dog (0.1%), Babesia canis was detected but there was no further information about the dogs origin. Evaluation of the questionnaire identified a high proportion of dogs (74.2%, n=233) which was not protected by ectoparasiticides. Moreover, 21.2% (n=236) of the dogs originated from inland or abroad shelters, and therefore might potentially come from areas endemic for dirofilariosis or babesiosis.
Methicillin-resistant staphylococcus aureus (MRSA) in meat and meat products results of a suivey study [Methicillin-resistente staphylococcus aureus in fleisch und fleischerzeugnissen: Ergebnisse einer übersichtsuntersuchung]
Schilling C.,Landeslabor Berlin Brandenburg |
Tenhagen B.-A.,Bundesinstitut For Risikobewertung |
Guerra B.,Bundesinstitut For Risikobewertung |
Fetsch A.,Bundesinstitut For Risikobewertung
Fleischwirtschaft | Year: 2010
In the course of the present study, we detected MRSA in 2.0% of the examined 844 samples of the categories raw meat, minced meat, meat preparations and raw sausage/raw pickled products. The detection was particularly successful from unseasoned minced meat wholly or partially made of pork. Most of the MRSA isolates (15/17) belonged to the clonal complex CC398. However, two of the strains isolated from minced meat were of a human-associated type with colonized personnel as the most likely source. The reason for the comparatively low detection rates is assumed to be the low sample weight of 1 g. Further investigations with the conventional sample weight of 25 g are needed to gain closer insights regarding the MRSA load of meat and meat products, especially of minced meat.
Rapid and simple determination of residues of the aniline-forming pesticides amitraz, vinclozolin and chlorpropham in plant-based foodstuffs and honey by means of HS-SPME and GC-MS according to residue definition [Schnelle und einfache Rückstandsbestimmung der anilinbildenden Pflanzenschutzmittel Amitraz, Vinclozolin und Chlorpropham in pflanzlichen Lebensmitteln und Honig durch HS-SPME und GC-MS gemäß Rückstandsdefinition]
Hemmerling C.,Lorbeerweg 22 |
Witt G.,Landeslabor Berlin Brandenburg
Deutsche Lebensmittel-Rundschau | Year: 2015
We describe development and validation of a method, which allows the determination of the total residues of the pesticides Amitraz, Vinclozolin, and Chorpropham, including their degradation products as required by the definition of residues, in phytogenic foodstuffs and honey. The effort in terms of work force and equipment needed to carry out these measurements is extremely small. The standard procedures based on steam distillation were replaced by performing the hydrolysis in a screws head-space vial overnight in a drying oven at 90°C. The extraction is carried out by headspace solid-phase extraction at 60°C for 30 min. Desorption happens in the injector of the gas Chromatograph. As detector any MSD or a GC-MS-MS are suitable. The method tested using pear, potato, kiwi, and honey matrices. The procedure shows a very good linearity for all four analytes over the range 10-2000 µg/kg, it is therefore perfectly suited for monitoring the statutory maximum levels. The recovery rates amounted to 88-100 [%] for adding 50 µg/kg to a pear matrix. The relative standard deviation of the measured values was 5-18 [%] with the MSD and 2-6 [%] with the GC-MS-MS. In diesem Beitrag kann nur eine Abbildung gezeigt werden. Weitere Chromatogramme (Kartoffel, Kiwi, Birne und Honig) können jedoch beim Autor eingesehen werden.
Determination of ochratoxin A in liquorice root by HPLC-FLD after solid-phase extraction with immunoaffinity columns [Bestimmung von ochratoxin a in süßholz mittels HPLC-FLD nach reinigung über immunoaffinitätssäulen]
Barricelli M.,Landeslabor Berlin Brandenburg |
Schmidt K.,Landeslabor Berlin Brandenburg |
Borner B.,Landeslabor Berlin Brandenburg
Deutsche Lebensmittel-Rundschau | Year: 2010
This paper describes a method of the determination of Ochratoxin A in Liquorice root by high-performance liquid chromatography using fluorescence detection after solid-phase extraction with immunoaffinity columns. Interference matrix was eliminated by treating the matrix extract with Dichloromethane before IAC cleanup.