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Lamphun, Thailand

Charnsil C.,Chiang Mai University | Sriapai P.,Lamphun Hospital
Journal of the Medical Association of Thailand | Year: 2011

Objective: (1) to examine the co-occurrence of attention deficit and hyperactivity symptoms in children with autistic disorder, and (2) to study the correlation between attention deficit hyperactivity symptoms and the severity of autistic disorder. Material and Method: This was a clinical based study. The authors used Childhood Autistic Rating scale (CARs) to evaluate the severity of autistic disorder. Swanson, Nolan, and Pelham Teacher and Parent Rating Scale, Version IV (SNAP-IV) was used to measure attention deficit and hyperactive symptoms in children with autism. Results: Thirty (n = 30) children enrolled in this study. All participants displayed attention deficit symptoms and 18 participants demonstrated hyperactivity as well. Nonparametric correlation showed a high positive correlation (Spa = 0.90, p = 0.00) between the severity of autistic disorder and hyperactivity and not the attention deficit symptoms (Spa = 0.29, p = 0.16). Conclusion: The authors finding shows a high comorbid rate of attention deficit and hyperactive symptoms among the participants. Source


Hanterdsith B.,Lamphun Hospital | Mahanupab P.,Chiang Mai University
American Journal of Forensic Medicine and Pathology | Year: 2010

A 20-year-old well nourished woman was found dead suddenly and unexpectedly in her bedroom. According to her medical history, Graves' disease was diagnosed in August 2000 and the symptoms of thyrotoxicosis were poorly controlled. Autopsy and histology revealed diffuse enlargement of the thyroid gland (125 g, diffuse hyperplastic goiter), pulmonary edema, cardiomegaly (440 g) without coronary artery occlusion or stenosis. The cardiac muscles showed interstitial fibrosis with hypertrophy of the myocardial fibers. The circumstances and scene of death as well as the detailed postmortem analysis indicated that thyroid crisis from Graves' disease was the cause of death. Copyright © 2010 by Lippincott Williams & Wilkins. Source


Pornprasert S.,Chiang Mai University | Jaiping K.,Lamphun Hospital
Hemoglobin | Year: 2014

A subject with Hb E (HBB: c.79G > A) trait is asymptomatic and can become a blood donor. However, a blood transfusion from a Hb E trait donor can affect β-thalassemia (β-thal) diagnosis. Blood samples from three Thai women were sent to the Associated Medical Sciences (AMS) Clinical Service Center, Chiang Mai, Thailand, for thalassemia diagnosis. Their Hb A2 levels, analyzed by high performance liquid chromatography (HPLC), were higher than 4.0%, thus they were diagnosed to have β-thal. However, elevated Hb A2 levels in these patients were not certain because the Hb A2 levels analyzed at the initial hospitalization and follow-up were controversial. In addition, there were some cases shown to have controversy between the increased Hb A2 level and red cell indices. The blood transfusion history was confirmed and hemoglobin (Hb) analysis was reanalyzed by capillary electrophoresis (CE). On the CE electrophoregram, Hb A2 levels were observed to be normal and Hb E peaks were present. Therefore, to rule out misdiagnosis and unnecessary genetic counseling, Hb analysis should be performed on the recipient prior to blood transfusions. Moreover, CE has a high efficiency to prevent the misinterpretation of Hb analysis in patients who receive blood transfusions from a donor carrying Hb E. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted. Source


Pornprasert S.,Chiang Mai University | Moriyama A.,Yamaguchi University | Kongthai K.,Health Promoting Hospital | Waneesorn J.,Regional Medical science Center 10 | And 3 more authors.
Clinical Laboratory | Year: 2013

Background: Differentiation of β-thalassemia/HbE disease from homozygous HbE in samples containing HbA2/E > 75% and HbF < 15% is difficult. The aim of this study is to observe the possibility of using Hb typing and hematological parameters to identify both disorders. Methods: Multiplex amplification refractory mutation system (MARMS)-PCR for β-thalassemia codons 17 (A > T), 41/42 (-TCTT), 71/72 (+A), and IVSI-nt1 (G > T) mutations and ARMS-PCR for HbE were performed in 67 samples that contained HbA2/E > 75% and HbF < 15%. Results: β-thalassemia/HbE disease was identified in 10 of 67 (14.93%) samples. Levels of hemoglobin, hematocrit, and mean corpuscular volume (MCV) of β-thalassemia/HbE disease were significantly lower than those of homozygous HbE whereas, levels of HbF were significantly higher. Conclusions: In places where the molecular analysis is not available, HbF > 5% in combination with MCV < 55 fL, hemoglobin < 100 g/L, and hematocrit < 0.30 L/L could be used for screening of β-thalassemia/HbE disease. Source


Pornprasert S.,Chiang Mai University | Tookjai M.,Chiang Mai University | Punyamung M.,Chiang Mai University | Pongpunyayuen P.,Chiang Mai University | Jaiping K.,Lamphun Hospital
Clinical Chemistry and Laboratory Medicine | Year: 2016

Background: To date, the hemoglobin (Hb) typing control materials for laboratory investigation of thalassemia with low (1.8%-3.2%) and high (4%-6%) levels of HbA2 are available but there are no Hb typing quality control materials for analysis of thalassemia and hemoglobinopathies which are highly prevalent in South-East Asian countries. The main aim of the present study was to develop the lyophilized Hb typing control materials for laboratory investigation of thalassemia and hemoglobinopathies that are commonly found in South-East Asia. Methods: Erythrocytes of blood samples containing Hb Bart's, HbH, HbE, HbF, Hb Constant Spring (CS), Hb Hope, and Hb Q-Thailand were washed and dialysed with 0.85% saline solution. The erythrocytes were then lysed in 5% sucrose solution. The lyophilized Hb typing control materials were prepared by using a freeze drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized Hb was performed after the storage at -20 °C for 1 year and also after reconstitution and storage at 4 or -20 °C for 30 days. In addition, the Hb analysis was compared between the three different methods of HPLC, low pressure liquid chromatography (LPLC) and capillary electrophoresis (CE). Results: Following a year of storage at -20 °C, the HPLC chromatograms of lyophilized Hb typing control materials showed similar patterns to the equivalent fresh whole blood. The stability of reconstituted Hb typing control materials was also observed through 30 days after reconstitution and storage at -20 °C. Moreover, the Hb typing control materials could be analyzed by three methods, HPLC, LPLC and CE. Even a degraded peak of HbCS was found on CE electropherogram. Conclusions: The lyophilized Hb typing control materials could be developed and used as control materials for investigation of thalassemia and hemoglobinopathies. © 2016 by De Gruyter 2016. Source

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