Lalahan Livestock Central Research Institute

Ankara, Turkey

Lalahan Livestock Central Research Institute

Ankara, Turkey

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Bucak M.N.,Selcuk University | Baspinar N.,Selcuk University | Tuncer P.B.,Lalahan Livestock Central Research Institute | Coyan K.,Selcuk University | And 4 more authors.
Andrologia | Year: 2012

The aim of this study was to determine the effects of curcumin and dithioerythritol added into bull semen extender on sperm parameters, lipid peroxidation, total glutathione and antioxidant potential levels of bull spermatozoa following the freeze/thawing process. Twenty-seven ejaculates obtained from three bulls were included in the study. Each ejaculate that was splitted into five equal groups and diluted in a Tris-based extender containing curcumin (0.5 and 2mm), dithioerythritol (0.5 and 2mm) and no additive (control) was cooled to 5°C and frozen in 0.25-ml French straws. The extender supplemented with 0.5mm dose of curcumin led to lower percentage of total abnormality (20.40±2.36%) when compared to the control (30.60±1.47%, P<0.05). Curcumin and dithioerythritol at 0.5mm provided a greater protective effect in the membrane functional integrity (54.40±2.09% and 50.00±2.68%), in comparison with control (37.20±1.77%, P<0.001). Supplementation with antioxidants did not significantly affect the lipid peroxidation and antioxidant potential levels, while the maintenance of total glutathione levels in curcumin 0.5mm was demonstrated to be higher than that of control, following the freeze/thawing (P<0.05). Supplementation with these antioxidants prior to the cryopreservation process may be recommended to facilitate the enhancement of sperm cryopreservation techniques. © 2011 Blackwell Verlag GmbH.


Tuncer P.B.,Lalahan Livestock Central Research Institute | Sarozkan S.,Erciyes University | Bucak M.N.,Lalahan Livestock Central Research Institute | Bucak M.N.,Selcuk University | And 4 more authors.
Theriogenology | Year: 2011

The objective of this study was to evaluate the effects of the addition of different sugars (raffinose, sucrose, and trehalose) on bull spermatozoa cryopreserved in a commercial extender (Optidyl) supplemented with glutamine on semen parameters, fertilizing ability and superoxide dismutase (SOD) activity. Nine ejaculates for each bull were used in the study. Semen was frozen in five different extenders: raffinose 25 mM plus glutamine 3 mM (RGO), sucrose 25 mM plus glutamine 3 mM (SGO), trehalose 25 mM plus glutamine 3 mM (TGO), glutamine 3 mM (GO) and control (O). Insemination doses were processed so that each 0.25 mL straw contained 15 x 106sperm. Groups of GO and RGO resulted in the higher rates of subjective (54.0 1.7% and 64.0 1.1%; P < 0.01) and CASA motilities (53.0 2.7% and 61.0 4.4%; P < 0.001), respectively compared to the other groups. The supplementation of additives did not provide an effect on the level of post-thaw sperm CASA progressive motilities, the sperm motion characteristics and pregnancy rates. GO and RGO provided the better protective effect for sperm acrosome (4.0 0.5% and 12.0 0.6%) and total abnormalities (5.0 0.3% and 13.0 0.7%; P < 0.001), respectively. At the HOST values, the additives did not give to result the protective effect in comparison to Optydil extender without additives (P > 0.05). For pregnancy rates, there were no significant differences among the groups. The supplementation of additives did not provide any significant difference on the level of SOD activity (P > 0.05). It can be also thought that these sugars might have worked with glutamine in a synergy. Thereby, sugars such as raffinose and sucrose with glutamine in freezing extender may be recommended to facilitate bull semen freezability. © 2011 Elsevier Inc.


Sariozkan S.,Erciyes University | Bucak M.N.,Lalahan Livestock Central Research Institute | Tuncer P.B.,Lalahan Livestock Central Research Institute | Tasdemir U.,Lalahan Livestock Central Research Institute | And 2 more authors.
Theriogenology | Year: 2010

The main objective of the current study was to evaluate the effects of extender type and centrifugation/washing prior to cryopreservation on the postthaw sperm parameters, lipid peroxidation, and superoxide dismutase activity of Angora buck (Capra hircus ancryrensis) sperm. Ejaculates collected from three Angora bucks were used in this study. Two consecutive ejaculates from each buck were pooled and split into equal parts in four Falcon tubes. Two tubes were diluted at 37 °C and then centrifuged to remove semen plasma. After centrifugation, two sediment parts were diluted with a Tris-based extender and commercial Bioxcell extender, respectively. The remaining two parts, which were not centrifuged/washed, were diluted with the above-mentioned extenders, respectively. Diluted samples were cooled to 5 °C and frozen in 0.25-mL French straws to be stored in liquid nitrogen. Frozen straws were thawed individually at 37 °C for 20 sec in a water bath for evaluation. The semen part with centrifugation/washing in the Bioxcell extender (BC) demonstrated a higher rate of subjective motility (58.1 ± 3.0%) compared with that of groups with (TC) or without (T) centrifugation/washing in the Tris-based extender (P < 0.01). Angora buck sperm frozen with (BC) or without (B) centrifugation/washing in the Bioxcell extender demonstrated higher percentages of motility (60.6 ± 2.7% and 54.3 ± 4.8%, respectively) compared with that of groups T and TC. The postthaw progressive motility rate (22.3 ± 2.7%) was significantly greater for semen parts diluted in B compared with that of other groups. BC gave rise to a lower value of average path velocity (90.0 ± 5.2 μm/sec) compared with that of other groups (P < 0.01). For straight linear velocity and linearity index, the highest values (103.2 ± 4.7 μm/sec, 47.5 ± 1.6% and 94.8 ± 3.0 μm/sec, 44.8 ± 1.1%, respectively) were obtained from B and TC (P < 0.001). For sperm acrosome and total abnormalities, TC gave the highest values (11.2 ± 0.6% and 26.6 ± 1.5%, respectively, P < 0.01). In the group frozen in BC, the percentage of membrane integrity assessed by hypo-osmotic swelling test was higher (61.2 ± 2.2%) than that of the other groups (P < 0.001). With respect to fertility results based on 35-d pregnancy rates, BC gave a higher rate (76.5%) than that of TC (27.8%, P < 0.05). Malondialdehyde formation was found to be lower (1.64 ± 0.26 nmol/L) in BC than in the other groups after the freeze-thawing process (P < 0.001). In the semen part frozen in BC, superoxide dismutase activity was higher (0.18 ± 0.02 U/mg protein) compared with that of the other groups (P < 0.05). Further studies are required to obtain more precise results for the characterization of oxidative stress parameters and fertilizing ability in cryopreserved buck spermatozoa. Crown Copyright © 2010.


Karakaya E.,Uludag University | Yilmazbas-Mecitoglu G.,Uludag University | Keskin A.,Uludag University | Alkan A.,TARFAS Co. | And 3 more authors.
Reproduction in Domestic Animals | Year: 2014

Contents: The aim of this study was to compare pregnancy per artificial insemination (P/AI) after timed AI with sex-sorted sperm (SS) or conventional semen (CS) in lactating dairy cows. Cyclic cows (n = 302) were synchronized by Ovsynch and randomly assigned into two groups at the time of AI. Cows with a follicle size between 12 and 18 mm and clear vaginal discharge at the time of AI were inseminated with either frozen-thawed SS (n = 148) or CS (n = 154) of the same bull. A shallow uterine insemination was performed into the uterine horn ipsilateral to the side of probable impending ovulation. Pregnancy per AI on Day 31 tended (p = 0.09) to be less for SS (31.8%) than CS (40.9%). Similarly, P/AI on Day 62 was less (p = 0.01) for cows inseminated with SS (25.7%) compared with CS (39.0%). The increased difference in fertility between treatments from Days 31 to 62 was caused by the greater (p = 0.02) pregnancy loss for cows receiving SS (19.2%) than CS (4.8%). Cow parity (p = 0.02) and season (p < 0.01) when AI was performed were additional factors affecting fertility. Primiparous cows had greater P/AI than multiparous cows both on Day 31 (41.7% vs 25.0% in SS and 53.0% vs 31.8% in CS groups) and on Day 62 (33.3% vs 20.5% in SS and 48.5% vs 31.8% in CS groups). During the hot season of the year, P/AI on Day 31 was reduced (p = 0.01) in the SS group (19.6%) when compared with the rates during the cool season (38.1%). In conclusion, sex-sorted sperm produced lower fertility results compared to conventional semen even after using some selection criteria to select most fertile cows. © 2014 Blackwell Verlag GmbH.


Tuncer P.B.,Lalahan Livestock Central Research Institute | Bucak M.N.,Lalahan Livestock Central Research Institute | Sariozkan S.,Erciyes University | Sakin F.,Mustafa Kemal University | And 6 more authors.
Cryobiology | Year: 2010

The aim of the present study was to determine the effects of different doses of raffinose and methionine on post-thawed semen quality, lipid peroxidation and antioxidant enzyme activities of Angora buck (Capra hircus ancryrensis) sperm following cryopreservation.Ejaculates collected from three Angora bucks were evaluated and pooled at 37 °C. Semen samples, which were diluted with a Tris-based extender containing the additives raffinose (2.5, 5, 10. mM) and methionine (2.5, 5, 10. mM) and an extender containing no antioxidants (control), were cooled to 5 °C and frozen in 0.25. ml French straws. Frozen straws were thawed individually at 37 °C for 20. s in a water bath for evaluation. The freezing extender supplemented with 2.5 and 5. mM methionine led to higher percentages of CASA motility (63.6 ± 7.0; 63.4 ± 3.1%, respectively), in comparison to the controls (P< 0.01) following the freeze-thawing process. The addition of antioxidants did not provide any significant effect on the percentages of post-thaw subjective and CASA progressive motilities as well as sperm motion characteristics (VSL and VCL), compared to the control groups (P> 0.05). The freezing extender with raffinose (5 and 10. mM) and methionine at three different doses (2.5, 5 and 10. mM) led to lower percentages of acrosome abnormalities, in comparison to the controls (P< 0.001). In the comet test, raffinose (5 and 10. mM) and methionine (10. mM) gave scores lower than those of the controls, and thereby reduced DNA damage (P< 0.05). Malondialdehyde formation was found to be lower (1.8 ± 0.1. nmol/L) in the group of 5. mM raffinose, compared to the controls following the freeze-thawing process (P< 0.01). The additives did not show any effectiveness on the maintenance of SOD, GSH-PX and GSH activities, when compared to the controls (P> 0.05). In conclusion, methionine and raffinose play a cryoprotective role against sperm CASA motility, acrosome abnormality and DNA damage. Raffinose 5. mM exhibited antioxidative properties, decreasing MDA levels. Further studies are required to obtain more concrete results on the characterization of microscopic parameters and antioxidant activities in cryopreserved goat sperm with different additives. © 2010.


Tasdemir U.,Lalahan Livestock Central Research Institute | Agaoglu A.R.,Mehmet Akif Ersoy University | Kaymaz M.,Ankara University | Karakas K.,Ankara University
Tropical Animal Health and Production | Year: 2011

The aim of this study was to compare ovarian response and embryo yield of Day 0 protocol in Angora goats (AG) and indigenous Kilis goats (KG) in the non-breeding season. A total of 16 Angora goats (AG group) and 11 Kilis goats (KG group) were used in this study. In the synchronization process, after controlled internal drug release withdrawal, when estrus signs were observed, natural mating was performed. Ovarian response was determined by synchronized laparotomy 6 days after natural mating, and number of corpora lutea (CL) was recorded. Embryos were collected and morphologically evaluated by stereomicroscope. Synchronization rates did not differ between AG (88%, 14/16) and KG group (91%, 10/11). In AG and KG groups, the proportion of CL on the right (44% and 53%, respectively) and left (56% and 47%, respectively) ovaries were similar. The CL number per animal did not differ significantly between the two breeds and was determined as 4.4 ± 0.90 in AG group and 6.4 ± 1.44 in KG group. Transferable embryo yields were significantly higher in AG group (31/42, 74%) compared to KG group (16/46, 35%) in the non-breeding season (P < 0.01). In conclusion, it is suggested that the day 0 protocol can be used for goat superovulation in the non-breeding season; however, transferable embryo yields are affected by the breed. © 2011 Springer Science+Business Media B.V.


Bucak M.N.,Lalahan Livestock Central Research Institute | Sariozkan S.,Erciyes University | Tuncer P.B.,Lalahan Livestock Central Research Institute | Sakin F.,Dicle University | And 3 more authors.
Small Ruminant Research | Year: 2010

The aim of this study was to determine the effects of the antioxidants curcumin, inositol and carnitine on microscopic seminal parameters, lipid peroxidation (LPO) and the antioxidant activities of sperm, following the freeze-thawing of Angora goat semen. Ejaculates were collected via artificial vagina from three Angora goats and microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including curcumin (2.5, 5 or 10 mM), inositol (2.5, 5 or 10 mM), carnitine (2.5, 5 or 10 mM) and no antioxidant (control). The diluted semen was slowly (at a rate of 0.2-0.3 °C/min) cooled to 5 °C and then cryopreserved in 0.25 mL French straws. Frozen straws were thawed individually at 37 °C for 20 s in a water bath, for microscopic sperm evaluation. The freezing extender supplemented with 2.5 mM curcumin led to higher percentage of computer-assisted semen analyzer (CASA) sperm motility (65 ± 3%), when compared to the control, inositol and the 10 mM carnitine (P < 0.01) groups, following the freeze-thawing process. The addition of antioxidants did not provide any significant effect on the percentages of post-thaw subjective analyses and CASA progressive motilities, as well as sperm motility characteristics (VAP, VSL, LIN and ALH), compared to the controls. Freezing extenders with antioxidants at three different doses led to lower percentages of acrosome and total sperm abnormalities, when compared to the controls (P < 0.001). However, the addition of 5 mM inositol did not induce any difference in total sperm abnormalities, when compared to the controls. The antioxidants also did not show any effectiveness in the elimination of malondialdehyde (MDA) formation and the maintenance of glutathione peroxidase (GSH-PX) activity, when compared to the controls. Superoxide dismutase (SOD) activity was found to be higher in the presence of curcumin at all three dose levels and carnitine at 5 mM, compared to the other groups. Glutathione (GSH) concentration was demonstrated to be maintained at a higher level with the addition of inositol, compared to the other groups. However, these differences in SOD and GSH levels were not significant, compared to the controls. All the antioxidants at all three dose levels resulted in a better protection of the sperm morphology (except for 5 mM inositol with respect to the total sperm abnormalities), compared to the control samples. According to CASA, the best post-thawing sperm motility rate was recorded when the freezing extender was supplemented with 2.5 mM curcumin. Further studies are required to obtain more conclusive results regarding the characterization of microscopic and oxidative stress parameters in cryopreserved goat sperm, using the different antioxidants. Crown Copyright © 2009.


Bayrak Z.,Gulhane Military Medical Academy | Tas C.,Gulhane Military Medical Academy | Tasdemir U.,Lalahan Livestock Central Research Institute | Erol H.,Lalahan Livestock Central Research Institute | And 3 more authors.
European Journal of Pharmaceutics and Biopharmaceutics | Year: 2011

First-pass metabolism can be overcome by sublingual drug delivery, and quick drug entry into the systemic circulation can be obtained. In certain diseases such as migraine therapy, taking fast pharmacological response is an important criteria. In this study, zolmitriptan sublingual tablets were prepared by direct compression method using different mucoadhesive polymers such as hydroxypropyl methyl cellulose, chitosan and sodium carboxy methyl cellulose at a concentration range of 0.5-5% to reduce flushing action of saliva and provide enough time for drug to be absorbed. Tablets were evaluated for the physical properties, and optimum formulations were chosen for in vivo studies to carry on sheep model. The tablets disintegrated rapidly, and dissolution tests revealed that zolmitriptan was dissolved from the formulation within the compendial limits. This especially showed us that the concentration range of polymers is in acceptable limit. It was also concluded that microcrystalline cellulose, spray-dried lactose and sodium starch glycolate are the appropriate excipient and formulated in good proportions. In vivo studies indicated that formulation containing 5% chitosan has the maximum C max and AUC and minimum t max values (p < 0.05). As a result, sublingual tablet administration of zolmitriptan formulated with appropriate excipients and especially with chitosan seems promising alternative to traditional routes. © 2011 Elsevier B.V. All rights reserved.


PubMed | Lalahan Livestock Central Research Institute
Type: Evaluation Studies | Journal: Tropical animal health and production | Year: 2011

The aim of this study was to compare ovarian response and embryo yield of Day0 protocol in Angora goats (AG) and indigenous Kilis goats (KG) in the non-breeding season. A total of 16 Angora goats (AG group) and 11 Kilis goats (KG group) were used in this study. In the synchronization process, after controlled internal drug release withdrawal, when estrus signs were observed, natural mating was performed. Ovarian response was determined by synchronized laparotomy 6days after natural mating, and number of corpora lutea (CL) was recorded. Embryos were collected and morphologically evaluated by stereomicroscope. Synchronization rates did not differ between AG (88%, 14/16) and KG group (91%, 10/11). In AG and KG groups, the proportion of CL on the right (44% and 53%, respectively) and left (56% and 47%, respectively) ovaries were similar. The CL number per animal did not differ significantly between the two breeds and was determined as 4.40.90 in AG group and 6.41.44 in KG group. Transferable embryo yields were significantly higher in AG group (31/42, 74%) compared to KG group (16/46, 35%) in the non-breeding season (P<0.01). In conclusion, it is suggested that the day0 protocol can be used for goat superovulation in the non-breeding season; however, transferable embryo yields are affected by the breed.


PubMed | Lalahan Livestock Central Research Institute
Type: Journal Article | Journal: Theriogenology | Year: 2011

The objective of this study was to evaluate the effects of the addition of different sugars (raffinose, sucrose, and trehalose) on bull spermatozoa cryopreserved in a commercial extender (Optidyl) supplemented with glutamine on semen parameters, fertilizing ability and superoxide dismutase (SOD) activity. Nine ejaculates for each bull were used in the study. Semen was frozen in five different extenders: raffinose 25 mM plus glutamine 3 mM (RGO), sucrose 25 mM plus glutamine 3 mM (SGO), trehalose 25 mM plus glutamine 3 mM (TGO), glutamine 3 mM (GO) and control (O). Insemination doses were processed so that each 0.25 mL straw contained 15 x 10(6)sperm. Groups of GO and RGO resulted in the higher rates of subjective (54.0 1.7% and 64.0 1.1%; P < 0.01) and CASA motilities (53.0 2.7% and 61.0 4.4%; P < 0.001), respectively compared to the other groups. The supplementation of additives did not provide an effect on the level of post-thaw sperm CASA progressive motilities, the sperm motion characteristics and pregnancy rates. GO and RGO provided the better protective effect for sperm acrosome (4.0 0.5% and 12.0 0.6%) and total abnormalities (5.0 0.3% and 13.0 0.7%; P < 0.001), respectively. At the HOST values, the additives did not give to result the protective effect in comparison to Optydil extender without additives (P > 0.05). For pregnancy rates, there were no significant differences among the groups. The supplementation of additives did not provide any significant difference on the level of SOD activity (P > 0.05). It can be also thought that these sugars might have worked with glutamine in a synergy. Thereby, sugars such as raffinose and sucrose with glutamine in freezing extender may be recommended to facilitate bull semen freezability.

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