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Shkoporov A.N.,Russian National Research Medical University | Chaplin A.V.,Russian National Research Medical University | Khokhlova E.V.,Russian National Research Medical University | Shcherbakova V.A.,Russian Academy of Sciences | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

Culture-based study of the faecal microbiome in two adult female subjects revealed the presence of two obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gramnegative bacterial strains that represent novel species. The first strain, designated 627T, was a fastidious, slow-growing, indole-positive bacterium with a non-fermentative type of metabolism. The strain was characterized by the production of acetic and succinic acids as metabolic end products, the prevalence of iso-C15 : 0 fatty acid and the presence of menaquinones MK-10 and MK-11. The DNA G+C content was found to be 56.6 mol%. The second strain, designated 177T, was capable of fermenting a rich collection of carbohydrate substrates, producing acetic acid as a terminal product. The strain was indole-negative and resistant to bile. The major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0(in a 1 : 1 ratio) and the predominant menaquinone was MK-11.The DNA G+C content was 37.8 mol%. A phylogenomic analysis of the draft genomes of strains 627T and 177T placed these bacteria in the genera Alistipes (family Rikenellaceae) and Coprobacter (family Porphyromonadaceae), respectively. On the basis of the phenotypic and genotypic properties of strains 627T and 177T, we conclude that these strains from human faeces represent two novel bacterial species, for which the names Alistipes inops sp. Nov. (type strain 627T=DSM 28863T=VKM B-2859T) and Coprobacter secundus sp. Nov. (type strain 177T=DSM 28864T=VKM B-2857T) are proposed. © 2015 IUMS. Source


Silachev D.N.,A N Belozersky Institute Of Physical And Chemical Biology | Plotnikov E.Y.,A N Belozersky Institute Of Physical And Chemical Biology | Babenko V.A.,lakov Research Center For Obstetrics | Zorova L.D.,Moscow State University | And 5 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2016

Here we studied the cytoprotective effect of lithium chloride and sodium valproate in the in vivo model of neonatal cerebral ischemia/hypoxia and analyzed the influence of these substances on the death of the major neurovascular unit components in experimental ischemia in vitro. Lithium chloride and sodium valproate effectively prevented death of neurons, astrocytes, and endothelial cells in the oxygen-glucose deprivation. This treatment protected the brain of newborn rats from ischemia/hypoxia injury. The results suggest that lithium and sodium valproate can be used for the treatment of neurodegenerative pathologies associated with hypoxia and ischemia in newborns. © 2016 Springer Science+Business Media New York Source


Shchyogolev A.I.,lakov Research Center For Obstetrics | Tumanova U.N.,lakov Research Center For Obstetrics | Dubova E.A.,lakov Research Center For Obstetrics
Bulletin of Experimental Biology and Medicine | Year: 2013

Comparative morphometric evaluation of the quantitative characteristics of vessels in hepatic focal nodular hyperplasia tissue was carried out on preparations stained with hematoxylin and eosin and by immunohistochemical detection of CD34 and CD105. Analysis of histological preparations stained with hematoxylin and eosin allowed the most ample characterization. Immunohistochemical detection of CD34 promoted better visualization of endotheliocytes, but just some sinusoids in the preparations were labeled. Expression of CD105 visualized the active endothelial cells and hence, helped to evaluate neoangiogenesis. © 2013 Springer Science+Business Media New York. Source


Gershtein E.S.,lakov Research Center For Obstetrics | Isaeva E.R.,okhin Russian Cancer Research Center | Kushlinsky D.N.,lakov Research Center For Obstetrics | Korotkova E.A.,okhin Russian Cancer Research Center | And 3 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2016

IGF-1, IGF-2, and IGFBP-1,2,3 were assayed in blood serum of patients with malignant ovarian tumors (n=44), borderline ovarian tumors (n=11), and benign ovarian tumors (n=12) as well as in healthy women (n=33). In blood serum of patients with malignant ovarian tumors, the level of IGF-1 was lower and IGFBP-1 was higher than in other groups. In patients with malignant and borderline ovarian tumors, the level of IGFBP-2 was higher than in healthy women and in patients with benign ovarian tumors. There was no correlation between most examined parameters and the clinical and morphological peculiarities of ovarian tumors. The study revealed IGF/IGFBP imbalance in patients with malignant ovarian tumor and showed that IGFBP-2 proved to be a potential diagnostic serological marker w with 90% sensitivity and 90% specificity. © 2016 Springer Science+Business Media New York Source


Dubova E.A.,lakov Research Center For Obstetrics | Pavlov K.A.,lakov Research Center For Obstetrics | Borovkova E.I.,Moscow State University | Bayramova M.A.,Moscow State University | And 2 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2011

Comparative morphological study of placentas from women with obesity and normal body weight was performed. Expression of vascular endothelial growth factor (VEGF) and its receptors (VEGFR-1, VEGFR-2, VEGFR-3) was detected by immunohistochemical methods. Nonbranching angiogenesis predominated in the placentas from obese women. Immunohistochemical analysis showed reduced intensity of the reaction to VEGF in the syncytiotrophoblast and vascular endothelium of stem villi and enhanced VEGF expression in non-villous cytotrophoblast and endothelial cells of capillaries of mature intermediate and terminal villi; reduced expression of VEGFR-1 and increased levels of VEGFR-2 and VEGFR-3 in the studied structures were also noted. © 2011 Springer Science+Business Media, Inc. Source

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