Laizhou Traditional Chinese Medicine Hospital

Weichanglu, China

Laizhou Traditional Chinese Medicine Hospital

Weichanglu, China
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Wang P.,Shenyang Pharmaceutical University | Wang B.,CAS Dalian Institute of Chemical Physics | Xu J.,Shenyang Pharmaceutical University | Sun J.,China Pharmaceutical University | And 4 more authors.
Journal of Chromatographic Science | Year: 2014

Ling-Gui-Zhu-Gan decoction (LGZGD), a well-known traditional Chinese medicine (TCM) formula, has been extensively used for the treatment of cardiovascular disease in clinic. However, the chemical constituents in LGZGD had not been investigated so far. In this study, an ultra performance liquid chromatography-hybrid electrospray ionization linear ion trap-Orbitrap mass spectrometry (UPLC-LTQOribitrap-MS/MS) method was established for rapid separation and structural identification of the constituents in LGZGD. Separation was performed on an ACQUITY™ UPLC BEH C18 column (50 × 2.1 mm, 1.7 μm) by gradient elution mode, using acetonitrile-water containing 0.1% formic acid as mobile phase at the flow rate of 0.2 mL/min. Accurate mass measurement for molecular ions and characteristic fragment ions could represent identification criteria for these compounds. As a result, 95 compounds including triterpene acids, triterpene saponins, flavonoids, coumarins, coumestans, benzofurans, phenylpropanoids and sesquiterpenoid lactones were detected, and 90 of them were tentatively identified. All compounds were further assigned in the individual raw material. In conclusion, the UPLCLTQ-Orbitrap-MS/MS is a highly efficient technique to separate and identify constituents in complex matrices of TCMs. These results obtained in this research will provide a basis for quality control and further in vivo study of LGZGD. © The Author 2014. Published by Oxford University Press. All rights reserved.


PubMed | Laizhou Traditional Chinese Medicine Hospital, China Pharmaceutical University, Shenyang Pharmaceutical University and CAS Dalian Institute of Chemical Physics
Type: Journal Article | Journal: Journal of chromatographic science | Year: 2015

Ling-Gui-Zhu-Gan decoction (LGZGD), a well-known traditional Chinese medicine (TCM) formula, has been extensively used for the treatment of cardiovascular disease in clinic. However, the chemical constituents in LGZGD had not been investigated so far. In this study, an ultra performance liquid chromatography-hybrid electrospray ionization linear ion trap-Orbitrap mass spectrometry (UPLC-LTQ-Oribitrap-MS/MS) method was established for rapid separation and structural identification of the constituents in LGZGD. Separation was performed on an ACQUITY(TM) UPLC BEH C18 column (50 2.1 mm, 1.7 m) by gradient elution mode, using acetonitrile-water containing 0.1% formic acid as mobile phase at the flow rate of 0.2 mL/min. Accurate mass measurement for molecular ions and characteristic fragment ions could represent identification criteria for these compounds. As a result, 95 compounds including triterpene acids, triterpene saponins, flavonoids, coumarins, coumestans, benzofurans, phenylpropanoids and sesquiterpenoid lactones were detected, and 90 of them were tentatively identified. All compounds were further assigned in the individual raw material. In conclusion, the UPLC-LTQ-Orbitrap-MS/MS is a highly efficient technique to separate and identify constituents in complex matrices of TCMs. These results obtained in this research will provide a basis for quality control and further in vivo study of LGZGD.


Ma D.-N.,Laizhou Traditional Chinese Medicine Hospital | Chi X.-C.,Laizhou Traditional Chinese Medicine Hospital | Song G.-Z.,Laizhou Traditional Chinese Medicine Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2010

The objective of this study was to explore the characteristics and treatment experience of cancer of the renal pelvis and ureter. The data of 31 cases of primary neoplasm of the renal pelvis and ureter were retrospectively analyzed. Most of the clinical manifestations were obvious hematuria or associated with urinary tract irritation, lumbago and hydronephrosis, whose main examinations were type-B ultrasonic, CT, contrast retrograde ureteropelvic and urine cytology. Twenty-eight cases were confirmed before surgery. Surgical excision was the main treatment. For the clinical manifestations of unexplained gross hematuria and lumbago, doctors should find out the reasons in time, have relevant examination and careful analysis, confirm the cases and have surgery as soon as possible.


Chi X.-C.,Laizhou Traditional Chinese Medicine Hospital | Chen Y.-G.,Laizhou Traditional Chinese Medicine Hospital | Qin L.-X.,Laizhou Traditional Chinese Medicine Hospital | Liu X.-L.,Laizhou Traditional Chinese Medicine Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2010

OBJECTIVE: To explore the indication and the procedure of reoperation for patients with locally recurrent rectal cancer. METHODS: A total of 58 patients with locally recurrent of rectal cancer and with reoperation were analyzed retrospectively from January 1998 to December 2004. RESULTS: Twenty patients underwent radical resection, 20 palliative resection and 10 patients colostomy. The 5-year survival rates of the radical, palliative and colostomy patients were 21.2%, 11.1% and 0 respectively. The median survival time was 42, 19 and 7 months respectively. CONCLUSION: The reoperation for the patients with locally recurrent of rectal cancer can improve the survival rate and quality of life.


Wang P.,Shenyang Pharmaceutical University | Sun J.,China Pharmaceutical University | Xu J.,Shenyang Pharmaceutical University | Yan Q.,Laizhou Traditional Chinese Medicine Hospital | And 4 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

Dictamnine is an herbal ingredient isolated from the root bark of Dictamnus dasycarpus Turcz. (Rutaceae). The present study was aimed at the development of an ultra-high performance liquid chromatography-tandem mass spectrometry method to quantify the concentration of dictamnine in rat plasma and tissues for the in vivo pharmacokinetics, tissue distribution and excretion study. Biological samples were processed with protein precipitation. Skimmianine was chosen as internal standard. The chromatographic separation was carried out on a Thermo Syncronis C18 column (2.1mm×50mm, 1.7μm) with an isocratic mobile phase consisting of methanol and 0.1% formic acid water (75:25, v/v). The detection was accomplished by using positive ion electrospray ionization in multiple reaction monitoring (MRM) mode. The MS/MS ion transitions were monitored at m/z 200.0→129.0 for dictamnine and 260.3→227.1 for IS, respectively. An excellent linearity was observed over the concentration range from 0.5 to 250ng/mL. The lower limit of quantification (LLOQ) was 0.5ng/mL for dictamnine. The developed method was rapid, accurate, and highly sensitive and selective. It was successfully applied to the in vivo pharmacokinetics, tissue distribution and excretion study of dictamnine in rats after oral or intravenous administration of dictamnine. © 2013 Elsevier B.V.

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