Yu W.,Qingdao University |
Guo F.,Laiwu City Peoples Hospital |
Song X.,Qingdao University
Pharmaceutical biology | Year: 2017
CONTEXT: Previous studies have reported that caveolin-1 (Cav-1) is associated with lung fibrosis. However, the role of Cav-1 expression in pirfenidone-treated idiopathic pulmonary fibrosis (IPF) is unknown.OBJECTIVE: This study investigated Cav-1 expression in pirfenidone-treated IPF, and compared the effects of pirfenidone with acetylcysteine and prednisone on IPF.MATERIALS AND METHODS: Rat IPF model was established by endotracheal injection of 5 mg/kg bleomycin A5 into the specific pathogen-free Wistar male rats. Pirfenidone (P, 100 mg/kg once daily), prednisone (H, 5 mg/kg once daily) and acetylcysteine (N, 4 mg/kg 3 times per day) were used to treat the rat model by intragastric administration for 45 consecutive days, respectively. The normal rats without IPF were used as the controls. After 15, 30 and 45 days of drug treatment, lung histopathology was assessed. The expression of Cav-1 was determined using real-time quantitative PCR and Western blot; the expression of tumour necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor (PDGF) was determined by enzyme-linked immunosorbent assay.RESULTS: After 15, 30 and 45 days of drug treatment, comparison of the three drug-treated groups with the model group showed significantly lower (p < 0.05) significance of airsacculitis and fibrosis scores of lung tissues, as well as expression of TGF-β1, TNF-α and PDGF, but the expression of Cav-1 was higher (p < 0.05). Compared with the N group, the fibrosis score was significantly lower and the protein expression of Cav-1 was significantly higher in the P group (p < 0.05). Additionally, the expression of Cav-1 was negatively correlated with the airsacculitis and fibrosis scores (r = -0.506, p < 0.01; r = -0.676, p < 0.01) as well as expression of TGF-β1, TNF-α and PDGF (r = -0.590, p < 0.01; r = -0.530, p < 0.01; r = -0.553, p < 0.01).DISCUSSION AND CONCLUSION: Pirfenidone, prednisone and acetylcysteine can inhibit airsacculitis and pulmonary fibrosis in rat IPF models, which may be related with enhanced caveolin-1, reduced TNF-α, TGF-β1, PDGF.
Gu J.,Laiwu City Peoples Hospital |
Hao C.,Maternal and Child Health Care Hospital of Laiwu |
Yan X.,Maternal and Child Health Care Hospital of Laiwu |
Xuan S.,Laiwu City Peoples Hospital
Oncology Letters | Year: 2017
In the present study, we evaluated the effects of ultra- sound-guided ilioinguinal and iliohypogastric nerve blocks and intratracheal general anesthesia in the radical surgery of aged cervical cancer. From June 2014 to December 2014, 62 patients diagnosed with aged cervical cancer were enrolled in this study. The patients conformed to the indication of cervical cancer radical surgery. Patients were randomly divided into the test group and the control group according to the random number table with 31 individuals in each group. General anesthesia and compound ultrasound-guided ilioinguinal and iliohypogastric nerve blocks were applied in the test group, while intratracheal general anesthesia was used in the control group. The results showed that the operative time, awake time and the total dosage of propofol and sufentanil in the test group were significantly lower than those in the control group. The postoperative VAS score, the times to press the automatic control bump and contin- uous application time of the automatic control bump in the test group were significantly less than those in the control group. The occurrence rate of anesthesia-related adverse reactions in the test group during the perioperative period was significantly lower than that in the control group. Differences were statistically significant (P<0.05). We concluded that ultrasound-guided ilioinguinal and iliohypogastric nerve blocks significantly improved the analgesic effects during the perioperative and postoperative period in cervical cancer radical surgeries. © 2017, Spandidos Publications. All rights reserved.
Lan F.,Tianjin Hospital |
Pan Q.,Laiwu City Peoples Hospital |
Yu H.,Peking University |
Yue X.,Tianjin Huanhu Hospital
Journal of Neurochemistry | Year: 2015
Temozolomide (TMZ) has been widely used in the treatment of glioblastoma (GBM), although inherent or acquired resistance restricts the application. This study was aimed to evaluate the efficacy of sulforaphane (SFN) to TMZ-induced apoptosis in GBM cells and the potential mechanism. Biochemical assays and subcutaneous tumor establishment were used to characterize the function of SFN in TMZ-induced apoptosis. Our results revealed that β-catenin and miR-21 were concordantly expressed in GBM cell lines, and SFN significantly reduced miR-21 expression through inhibiting the Wnt/β-catenin/TCF4 pathway. Furthermore, down-regulation of miR-21 enhanced the pro-apoptotic efficacy of TMZ in GBM cells. Finally, we observed that SFN strengthened TMZ-mediated apoptosis in a miR-21-dependent manner. In conclusion, SFN effectively enhances TMZ-induced apoptosis by inhibiting miR-21 via Wnt/β-catenin signaling in GBM cells. These findings support the use of SFN for potential therapeutic approach to overcome TMZ resistance in GBM treatment. © 2015 International Society for Neurochemistry.
Li F.,Shandong University |
Xu J.,Laiwu City Peoples Hospital |
Zheng J.,Shandong University |
Sokolove J.,Stanford University |
And 6 more authors.
Scientific Reports | Year: 2014
The aim of this study was to investigate the possible association in the interleukin-6 (IL-6) gene with Rheumatoid arthritis (RA) in Chinese Han population from Shandong Province. Target regions of IL-6 gene were amplified by polymerase chain reaction (PCR) and genotyped. A logistic regression analysis was performed to detect potential associations in our case-control sample, the odd ratio(OR) and 95% confidence intervals(CIs) were calculated. Furthermore, we systematically tracked all the published studies in the field and performed a meta-analysis for the single nucleotide polymorphisms (SNPs) under study. 256 RA patients and 331 healthy controls were recruited into the case-control study. We found allele frequencies of rs1800795, rs1800797 and rs1474347 in RA patients differ from control subjects (P = 0.016, 0.024, 0.020, respectively). Significant difference was observed in haplotype frequencies of GCCGCT between RA patients and controls (P = 0.0001, OR = 4.066, 95%CI = 1.891 ∼ 8.746), while GGCGCT frequencies was found lower in RA than controls (P = 0.006, OR = 0.669, 95%CI = 0.501 ∼ 0.894). The results of the meta-analysis showed association polymorphism within the IL-6 promoter with RA. These findings suggest that rare IL-6 gene polymorphisms may associate with RA susceptibility in Han Chinese populations; however further studies are needed to assess the validity of the association of IL-6 with RA.
Zhang R.,Jinan Military General Hospital |
Liu K.,Jinan Military General Hospital |
Yang L.,Jinan Maternity and Child Care Hospital |
Zhou T.,Laiwu City Peoples Hospital |
And 7 more authors.
European Radiology | Year: 2014
Objectives: The purpose of this investigation was to examine the association between cognition disorders and microstructural white matter (WM) changes in maintenance hemodialysis end-stage renal disease (ESRD) patients. Methods: Twenty-six maintenance hemodialysis ESRD patients and 28 healthy controls underwent diffusion tensor imaging (DTI), Mini Mental State Examination (MMSE), Trial Marking Test-A&B (TMT-A&B), and white matter hyperintensity (WMH) assessment. Tract-based spatial statistics (TBSS) analyses was performed to evaluate WM changes in the patients. Relationships between behavioural performances, clinical data, and the DTI index were tested, respectively, by correlation analysis at the voxel level. Results: ESRD patients showed significant decreased fractional anisotropy (FA) in 14 WM regions, and increased mean diffusivity (MD) and radial diffusivity (RD) in widespread regions. Significant positive correlations between FA values and MMSE scores were found in the right anterior corona radiata and the left anterior thalamic radiation; significant negative correlations between the TMT-B time consumption and FA values were identified in the bilateral superior longitudinal fasciculus. Positive linear relationships between MD, RD values, and the duration of hemodialysis were found in several WM regions. Conclusion: Structural damages to radiation and associative fibre tracts, caused by brain oedema and WM demyelination, may account for the cognitive deficits in ESRD patients. Key Points: • Lower FA, higher MD and RD values were found in ESRD patients. • Cognitive deficits in ESRD patients mainly focused on executive dysfunction. • Cognitive impairment in ESRD patients may be associated with damages to WM. • MD and RD are valuable in monitoring WM alteration in ESRD patients. © 2014, European Society of Radiology.
PubMed | Laiwu City Peoples Hospital and Qingdao University
Type: Journal Article | Journal: Pharmaceutical biology | Year: 2016
Previous studies have reported that caveolin-1 (Cav-1) is associated with lung fibrosis. However, the role of Cav-1 expression in pirfenidone-treated idiopathic pulmonary fibrosis (IPF) is unknown.This study investigated Cav-1 expression in pirfenidone-treated IPF, and compared the effects of pirfenidone with acetylcysteine and prednisone on IPF.Rat IPF model was established by endotracheal injection of 5mg/kg bleomycin A5 into the specific pathogen-free Wistar male rats. Pirfenidone (P, 100mg/kg once daily), prednisone (H, 5mg/kg once daily) and acetylcysteine (N, 4mg/kg 3 times per day) were used to treat the rat model by intragastric administration for 45 consecutive days, respectively. The normal rats without IPF were used as the controls. After 15, 30 and 45 days of drug treatment, lung histopathology was assessed. The expression of Cav-1 was determined using real-time quantitative PCR and Western blot; the expression of tumour necrosis factor- (TNF-), transforming growth factor-1 (TGF-1) and platelet-derived growth factor (PDGF) was determined by enzyme-linked immunosorbent assay.After 15, 30 and 45 days of drug treatment, comparison of the three drug-treated groups with the model group showed significantly lower (p<0.05) significance of airsacculitis and fibrosis scores of lung tissues, as well as expression of TGF-1, TNF- and PDGF, but the expression of Cav-1 was higher (p<0.05). Compared with the N group, the fibrosis score was significantly lower and the protein expression of Cav-1 was significantly higher in the P group (p<0.05). Additionally, the expression of Cav-1 was negatively correlated with the airsacculitis and fibrosis scores (r=-0.506, p<0.01; r=-0.676, p<0.01) as well as expression of TGF-1, TNF- and PDGF (r=-0.590, p<0.01; r=-0.530, p<0.01; r=-0.553, p<0.01).Pirfenidone, prednisone and acetylcysteine can inhibit airsacculitis and pulmonary fibrosis in rat IPF models, which may be related with enhanced caveolin-1, reduced TNF-, TGF-1, PDGF.
PubMed | Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Laiwu City Peoples Hospital and Shandong University
Type: Journal Article | Journal: Oncology reports | Year: 2016
The present study aimed to evaluate whether bromodomain4 (BRD4) is expressed in Cal27 cells and to assess the effect of JQ1 on cell proliferation, apoptosis, invasion and BRD4, C-Myc and Twist expression in Cal27 cells. Immunofluorescence staining was used to determine whether BRD4 was expressed in Cal27 cells. Cell viability and proliferation were evaluated using CCK-8 assay. Flow cytometry was used to determine the apoptosis and cell cycle distribution. The cell invasion was evaluated using Transwell plate. The expression levels of BRD4, C-Myc and Twist were determined by quantitative RT-PCR (qRT-PCR) and western blotting. BRD4 was highly expressed in Cal27 cells. JQ1 inhibited cell proliferation, induced cell apoptosis, induced cell cycle arrest, and inhibited cell invasion. Gene and protein expression levels of BRD4, C-Myc and Twist were downregulated in cells treated with JQ1. JQ1 inhibited Cal27 cell growth and invasion, and downregulated expression of several oncogenes. JQ1 may be a new drug for oral squamous cell carcinoma treatment.
Geng L.,Laiwu City Peoples Hospital |
Deng Y.,Laiwu City Peoples Hospital |
Wang Z.-Q.,Laiwu City Peoples Hospital
Cancer Research and Clinic | Year: 2013
Objective: To investigate the expressions of EGFR, Her-2 and VEGF in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma, as well as its clinic value of carcinogenesis, progression and metastasis in cervical squamous cell carcinoma. Methods: EGFR, Her-2 and VEGF were evaluated by immunohistochemistry in tissues from normal cervix, CIN and squamous cell carcinoma. Results: The expressions of EGFR, Her-2 and VEGF were negative in normal cervix. The positive rate of EGFR, Her-2 and VEGF in CIN tissues were 35.0% (14/40), 37.5% (15/40), 42.5% (17/40), while in cervical carcinoma tissues were 70.0% (35/50), 78.0% (39/50), 76.0% (38/50) respectively, which had statistical significance. There was relationship between the expressions of EGFR, Her-2, VEGF proteins with pathological grade andlymph node metastasis of squamous cell carcinoma. The expressions of Her-2 and VEGF correlated with the clinical stage of quamous cell carcinoma. Conclusion: The expressions of EGFR, Her-2 and VEGF may be correlated with the occurrence, development and metastasis of cervical carcinoma. It may be used as an important marker to evaluate the malignant degree of cervical carcinoma and it can be helpful in the treatment of cervical carcinoma.
PubMed | Laiwu City Peoples Hospital and Laicheng District Fangxia Hospital
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016
The aim of the present study was to investigate the expression levels of transforming growth factor (TGF)-1 and microRNA (miRNA)-145 in patients with diabetic foot ulcers (DFUs). A total of 26 patients with DFUs requiring amputation were enrolled in the study between January 2013 and August 2014. In addition, 15 trauma patients undergoing amputation over the same time period were included as a control group. Samples were collected from the blood, the dorsalis pedis arteries and muscles of the amputated limbs. The expression levels of TGF-1 mRNA and miRNA-145 in these samples was detected using reverse transcription-quantitative polymerase chain reaction. The expression levels of TGF-1 protein were evaluated using western blot analysis. In comparison with the control, the protein and mRNA expression levels of TGF-1 in the DFU patients was significantly higher in the serum and the dorsalis pedis arteries, and significantly lower in the muscles with ulcers. In contrast, the expression levels of miRNA-145 was significantly lower in the blood and the dorsalis pedis arteries, and significantly higher in the muscles with ulcers in DFU patients compared with the control. The results of the present study suggested that there exists an inverse correlation between the expression levels of miRNA-145 and TGF-1 in patients with DFU; thus suggesting that miRNA-145 may regulate the expression of TGF-1 in patients with DFUs.
Ren Y.,Laiwu City Peoples Hospital |
Ma G.,Laiwu City Peoples Hospital |
Peng L.,Laiwu City Peoples Hospital |
Ren Y.,Fangxia Hospital of Laicheng District |
Zhang F.,Laiwu City Peoples Hospital
Cell Biochemistry and Biophysics | Year: 2014
Our objective is to determine if actively screen the multi-drug resistant bacteria (MDRB) infection in intensive care unit (ICU) to prevent, control, and decrease the infection rate and transmission of MDRB. The patients admitted in ICU of one hospital in 2013 were analyzed. The throat swab, blood, defecation, and urine of patients were actively collected for bacteria cultures to screen Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii in patients. All patients received screening of MDRB infection and colonization within 2 days and after 2 days of admission, the results showed that there were 418 infectious bacterial strains in total and P. aeruginosa was the main bacterium. The asymptomatic infection rates of P. aeruginosa, K. pneumonia, E. coli, S. aureus, and A. baumannii were 39.02, 24.74, 44.00, 29.17, and 33.33 %, respectively; the symptomatic infection rates were 60.98, 75.26, 56.00, 70.83, and 66.67 %. 59.70 % patients received antibiotics treatment, 27.45 % patients received trachea cannula, 32.95 % patients received mechanism ventilation, 2.27 % patients received arterial cannula or venous cannula and 4.00 % patients received indwelling urinary catheters. The main MDRB in ICU is P. aeruginosa. The active screening of MDRB infection and colonization can provide the opportunity to take the life-saving measure against MDRB and treat patients. This can decrease the infection risk and the nosocomial transmission of MDRB. © 2014, Springer Science+Business Media New York.