Laiwu City Peoples Hospital
Laiwu City Peoples Hospital
Yu W.,Qingdao University |
Guo F.,Laiwu City Peoples Hospital |
Song X.,Qingdao University
Pharmaceutical biology | Year: 2017
CONTEXT: Previous studies have reported that caveolin-1 (Cav-1) is associated with lung fibrosis. However, the role of Cav-1 expression in pirfenidone-treated idiopathic pulmonary fibrosis (IPF) is unknown.OBJECTIVE: This study investigated Cav-1 expression in pirfenidone-treated IPF, and compared the effects of pirfenidone with acetylcysteine and prednisone on IPF.MATERIALS AND METHODS: Rat IPF model was established by endotracheal injection of 5 mg/kg bleomycin A5 into the specific pathogen-free Wistar male rats. Pirfenidone (P, 100 mg/kg once daily), prednisone (H, 5 mg/kg once daily) and acetylcysteine (N, 4 mg/kg 3 times per day) were used to treat the rat model by intragastric administration for 45 consecutive days, respectively. The normal rats without IPF were used as the controls. After 15, 30 and 45 days of drug treatment, lung histopathology was assessed. The expression of Cav-1 was determined using real-time quantitative PCR and Western blot; the expression of tumour necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor (PDGF) was determined by enzyme-linked immunosorbent assay.RESULTS: After 15, 30 and 45 days of drug treatment, comparison of the three drug-treated groups with the model group showed significantly lower (p < 0.05) significance of airsacculitis and fibrosis scores of lung tissues, as well as expression of TGF-β1, TNF-α and PDGF, but the expression of Cav-1 was higher (p < 0.05). Compared with the N group, the fibrosis score was significantly lower and the protein expression of Cav-1 was significantly higher in the P group (p < 0.05). Additionally, the expression of Cav-1 was negatively correlated with the airsacculitis and fibrosis scores (r = -0.506, p < 0.01; r = -0.676, p < 0.01) as well as expression of TGF-β1, TNF-α and PDGF (r = -0.590, p < 0.01; r = -0.530, p < 0.01; r = -0.553, p < 0.01).DISCUSSION AND CONCLUSION: Pirfenidone, prednisone and acetylcysteine can inhibit airsacculitis and pulmonary fibrosis in rat IPF models, which may be related with enhanced caveolin-1, reduced TNF-α, TGF-β1, PDGF.
Gu J.,Laiwu City Peoples Hospital |
Hao C.,Maternal and Child Health Care Hospital of Laiwu |
Yan X.,Maternal and Child Health Care Hospital of Laiwu |
Xuan S.,Laiwu City Peoples Hospital
Oncology Letters | Year: 2017
In the present study, we evaluated the effects of ultra- sound-guided ilioinguinal and iliohypogastric nerve blocks and intratracheal general anesthesia in the radical surgery of aged cervical cancer. From June 2014 to December 2014, 62 patients diagnosed with aged cervical cancer were enrolled in this study. The patients conformed to the indication of cervical cancer radical surgery. Patients were randomly divided into the test group and the control group according to the random number table with 31 individuals in each group. General anesthesia and compound ultrasound-guided ilioinguinal and iliohypogastric nerve blocks were applied in the test group, while intratracheal general anesthesia was used in the control group. The results showed that the operative time, awake time and the total dosage of propofol and sufentanil in the test group were significantly lower than those in the control group. The postoperative VAS score, the times to press the automatic control bump and contin- uous application time of the automatic control bump in the test group were significantly less than those in the control group. The occurrence rate of anesthesia-related adverse reactions in the test group during the perioperative period was significantly lower than that in the control group. Differences were statistically significant (P<0.05). We concluded that ultrasound-guided ilioinguinal and iliohypogastric nerve blocks significantly improved the analgesic effects during the perioperative and postoperative period in cervical cancer radical surgeries. © 2017, Spandidos Publications. All rights reserved.
Li Q.,Laiwu City Peoples Hospital |
Guo J.,Shouguang City Peoples Hospital |
Wang W.,Liaocheng Third Peoples Hospital |
Wang D.,Tongde Hospital of Zhejiang
Oncology Letters | Year: 2017
The expression of O6-methyl guanine DNA meth-yltransferase (MGMT) in different grade gliomas were analyzed in relation to its therapeutic effect and impact on disease prognosis. In total, 62 patients with glioma, who were admitted by neurosurgery and received surgical treatment and postoperative conventional chemoradiation, were selected for this study. Expression of MGMT was greater with an increase in brain glioma grade. Gender, age, tumor size and Karnofsky performance status (KPS) score did not differ with MGMT expression (P>0.05). Expression of MGMT in normal brain tissue was slightly significantly different than expression of MGMT in glioma tissue (P<0.05). The short-term efficacy and survival time of the MGMT-negative expression group were better than those of MGMT-positive expression. MGMT was only treated as an index to monitor tumor recurrence or metastasis and a reference to judge the prognosis of patients. The expression level of MGMT in glioma had no relation with age, gender, tumor size, surgical approach and KPS score. For glioma patients with positive expression of MGMT, antineo-plastic drugs of alkylating agent class should be avoided. © Spandidos Publications 2016. All rights reserved.
Ma M.,Laiwu City Peoples Hospital |
Zhai C.-X.,Laiwu City Peoples Hospital |
Sun C.-X.,Jilin University
Genetic Testing and Molecular Biomarkers | Year: 2017
Objective: This case-control study explored correlations between LP-PLA2 gene polymorphisms (A379V, V279F, and R92H) and susceptibility and severity of acute pancreatitis (AP) in a Chinese population. Materials and Methods: From October 2013 to October 2015, 94 AP patients were chosen as the case group. According to the Acute Physiology and Chronic Health Evaluation (APACHE) II score standard, AP patients were divided into a mild AP (MAP) group (n = 46) and severe AP (SAP) group (n = 48). The 48 SAP patients were further divided into an SAP with multiple organ dysfunction syndrome (MODS) group (n = 42) and SAP without MODS group (n = 6). Meanwhile, 96 healthy subjects who received physical examinations at the study hospitals were selected as the control group. Serum lipoprotein-associated phospholipase A2 (LP-PLA2) levels were detected by an enzyme-linked immunosorbent assay (ELISA). The A379V (s1051931), V279F (rs16874954), and R92H (rs13989) polymorphisms of the LP-PLA2 gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: There were significant differences in the frequencies of the LP-PLA2 gene polymorphisms between the AP group and the control group. The distribution of V279F-AA+AC genotype and R92H-AA+AG genotype in the AP group was higher than that in the control group, whereas the SAP group and SAP with MODS group distributions were higher than those in the MAP group and SAP without MODS group (both p < 0.05). G-C-A, G-A-G, and G-C-G haploids formed by A379V, V279F, and R92H may be associated with AP susceptibility. LP-PLA2 gene polymorphisms could affect serum LP-PLA2 level, whereas the V279F-A allele gene, the R92H-A allele gene, serum LP-PLA2 level, and serum amylase may be independent risk factors for AP (all p < 0.05). Conclusion: These results demonstrated that the LP-PLA2 gene polymorphisms, V279F and R92H, may be associated with susceptibility to and severity of AP. © Mary Ann Liebert, Inc. 2017.
Jia Z.-M.,Laiwu City Peoples Hospital
European review for medical and pharmacological sciences | Year: 2017
OBJECTIVE: We studied the influence of Dexmedetomidine on cognitive function in children during the recovery period of general anesthesia.PATIENTS AND METHODS: Ninety-three children who underwent general anesthesia were selected and randomly divided into (1) the control group, (2) the dexmedetomidine group, and (3) the dezocine group. Fentanyl, propofol, and rocuronium were used in all patients to induce anesthesia, while sevoflurane inhalation and propofol were used to maintain anesthesia. In the control group, 20 ml NS were infused intravenously 10 min before anesthetic induction. In the dexmedetomidine group, 1.0 μg/kg dexmedetomidine in 20 ml was infused for 10 min. In the dezocine group, 0.1 mg/kg dezocine in 20 ml was infused for 10 min. Mean arterial blood pressure, average heart rate, and average oxygen saturation (SaO2) were compared at the following time points: end of surgery (T0), before extubation (T1), during extubation (T2), and 30 min after extubation (T3). The VAS scale, Ramsay sedation score, delirium grading scale and occurrence of adverse reactions at 30 min after extubation were recorded. The occurrence of postoperative cognitive dysfunction (POCD) and the expression of serum neuron-specific enolase (NSE) and IL-6 at postoperative days 1 and 7 were recorded.RESULTS: Comparing mean arterial blood pressure, average heart rate, and average oxygen saturation (SaO2) at the different time points in the dexmedetomidine group, there were no statistically significant differences (p>0.05). The difference in the occurrence of adverse reactions in the different groups was statistically significant (p<0.05). The occurrence of postoperative cognitive dysfunction (POCD) at postoperative day 1 was significantly higher in the control group than the other two groups (p<0.05), and on the postoperative day 7th, the differences were not statistically significant (p>0.05). Regarding the expression of neuron-specific enolase (NSE) and IL-6, the levels were the highest in the control group, followed by the dezocine group (p<0.05).CONCLUSIONS: The dexmedetomidine is safer than dezocine in aspects of hemodynamics, sedation, analgesia, degree of delirium, occurrence of adverse reactions, and postoperative cognitive dysfunction (POCD). The improvement in the occurrence of postoperative cognitive dysfunction (POCD) is related to the levels of serum neuron-specific enolase (NSE) and IL-6.
Lan F.,Tianjin Hospital |
Pan Q.,Laiwu City Peoples Hospital |
Yu H.,Peking University |
Yue X.,Tianjin Huanhu Hospital
Journal of Neurochemistry | Year: 2015
Temozolomide (TMZ) has been widely used in the treatment of glioblastoma (GBM), although inherent or acquired resistance restricts the application. This study was aimed to evaluate the efficacy of sulforaphane (SFN) to TMZ-induced apoptosis in GBM cells and the potential mechanism. Biochemical assays and subcutaneous tumor establishment were used to characterize the function of SFN in TMZ-induced apoptosis. Our results revealed that β-catenin and miR-21 were concordantly expressed in GBM cell lines, and SFN significantly reduced miR-21 expression through inhibiting the Wnt/β-catenin/TCF4 pathway. Furthermore, down-regulation of miR-21 enhanced the pro-apoptotic efficacy of TMZ in GBM cells. Finally, we observed that SFN strengthened TMZ-mediated apoptosis in a miR-21-dependent manner. In conclusion, SFN effectively enhances TMZ-induced apoptosis by inhibiting miR-21 via Wnt/β-catenin signaling in GBM cells. These findings support the use of SFN for potential therapeutic approach to overcome TMZ resistance in GBM treatment. © 2015 International Society for Neurochemistry.
Li F.,Shandong University |
Xu J.,Laiwu City Peoples Hospital |
Zheng J.,Shandong University |
Sokolove J.,Stanford University |
And 6 more authors.
Scientific Reports | Year: 2014
The aim of this study was to investigate the possible association in the interleukin-6 (IL-6) gene with Rheumatoid arthritis (RA) in Chinese Han population from Shandong Province. Target regions of IL-6 gene were amplified by polymerase chain reaction (PCR) and genotyped. A logistic regression analysis was performed to detect potential associations in our case-control sample, the odd ratio(OR) and 95% confidence intervals(CIs) were calculated. Furthermore, we systematically tracked all the published studies in the field and performed a meta-analysis for the single nucleotide polymorphisms (SNPs) under study. 256 RA patients and 331 healthy controls were recruited into the case-control study. We found allele frequencies of rs1800795, rs1800797 and rs1474347 in RA patients differ from control subjects (P = 0.016, 0.024, 0.020, respectively). Significant difference was observed in haplotype frequencies of GCCGCT between RA patients and controls (P = 0.0001, OR = 4.066, 95%CI = 1.891 ∼ 8.746), while GGCGCT frequencies was found lower in RA than controls (P = 0.006, OR = 0.669, 95%CI = 0.501 ∼ 0.894). The results of the meta-analysis showed association polymorphism within the IL-6 promoter with RA. These findings suggest that rare IL-6 gene polymorphisms may associate with RA susceptibility in Han Chinese populations; however further studies are needed to assess the validity of the association of IL-6 with RA.
Zhang R.,Jinan Military General Hospital |
Liu K.,Jinan Military General Hospital |
Yang L.,Jinan Maternity and Child Care Hospital |
Zhou T.,Laiwu City Peoples Hospital |
And 7 more authors.
European Radiology | Year: 2014
Objectives: The purpose of this investigation was to examine the association between cognition disorders and microstructural white matter (WM) changes in maintenance hemodialysis end-stage renal disease (ESRD) patients. Methods: Twenty-six maintenance hemodialysis ESRD patients and 28 healthy controls underwent diffusion tensor imaging (DTI), Mini Mental State Examination (MMSE), Trial Marking Test-A&B (TMT-A&B), and white matter hyperintensity (WMH) assessment. Tract-based spatial statistics (TBSS) analyses was performed to evaluate WM changes in the patients. Relationships between behavioural performances, clinical data, and the DTI index were tested, respectively, by correlation analysis at the voxel level. Results: ESRD patients showed significant decreased fractional anisotropy (FA) in 14 WM regions, and increased mean diffusivity (MD) and radial diffusivity (RD) in widespread regions. Significant positive correlations between FA values and MMSE scores were found in the right anterior corona radiata and the left anterior thalamic radiation; significant negative correlations between the TMT-B time consumption and FA values were identified in the bilateral superior longitudinal fasciculus. Positive linear relationships between MD, RD values, and the duration of hemodialysis were found in several WM regions. Conclusion: Structural damages to radiation and associative fibre tracts, caused by brain oedema and WM demyelination, may account for the cognitive deficits in ESRD patients. Key Points: • Lower FA, higher MD and RD values were found in ESRD patients. • Cognitive deficits in ESRD patients mainly focused on executive dysfunction. • Cognitive impairment in ESRD patients may be associated with damages to WM. • MD and RD are valuable in monitoring WM alteration in ESRD patients. © 2014, European Society of Radiology.
Geng L.,Laiwu City Peoples Hospital |
Deng Y.,Laiwu City Peoples Hospital |
Wang Z.-Q.,Laiwu City Peoples Hospital
Cancer Research and Clinic | Year: 2013
Objective: To investigate the expressions of EGFR, Her-2 and VEGF in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma, as well as its clinic value of carcinogenesis, progression and metastasis in cervical squamous cell carcinoma. Methods: EGFR, Her-2 and VEGF were evaluated by immunohistochemistry in tissues from normal cervix, CIN and squamous cell carcinoma. Results: The expressions of EGFR, Her-2 and VEGF were negative in normal cervix. The positive rate of EGFR, Her-2 and VEGF in CIN tissues were 35.0% (14/40), 37.5% (15/40), 42.5% (17/40), while in cervical carcinoma tissues were 70.0% (35/50), 78.0% (39/50), 76.0% (38/50) respectively, which had statistical significance. There was relationship between the expressions of EGFR, Her-2, VEGF proteins with pathological grade andlymph node metastasis of squamous cell carcinoma. The expressions of Her-2 and VEGF correlated with the clinical stage of quamous cell carcinoma. Conclusion: The expressions of EGFR, Her-2 and VEGF may be correlated with the occurrence, development and metastasis of cervical carcinoma. It may be used as an important marker to evaluate the malignant degree of cervical carcinoma and it can be helpful in the treatment of cervical carcinoma.
Ren Y.,Laiwu City Peoples Hospital |
Ma G.,Laiwu City Peoples Hospital |
Peng L.,Laiwu City Peoples Hospital |
Ren Y.,Fangxia Hospital of Laicheng District |
Zhang F.,Laiwu City Peoples Hospital
Cell Biochemistry and Biophysics | Year: 2014
Our objective is to determine if actively screen the multi-drug resistant bacteria (MDRB) infection in intensive care unit (ICU) to prevent, control, and decrease the infection rate and transmission of MDRB. The patients admitted in ICU of one hospital in 2013 were analyzed. The throat swab, blood, defecation, and urine of patients were actively collected for bacteria cultures to screen Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii in patients. All patients received screening of MDRB infection and colonization within 2 days and after 2 days of admission, the results showed that there were 418 infectious bacterial strains in total and P. aeruginosa was the main bacterium. The asymptomatic infection rates of P. aeruginosa, K. pneumonia, E. coli, S. aureus, and A. baumannii were 39.02, 24.74, 44.00, 29.17, and 33.33 %, respectively; the symptomatic infection rates were 60.98, 75.26, 56.00, 70.83, and 66.67 %. 59.70 % patients received antibiotics treatment, 27.45 % patients received trachea cannula, 32.95 % patients received mechanism ventilation, 2.27 % patients received arterial cannula or venous cannula and 4.00 % patients received indwelling urinary catheters. The main MDRB in ICU is P. aeruginosa. The active screening of MDRB infection and colonization can provide the opportunity to take the life-saving measure against MDRB and treat patients. This can decrease the infection risk and the nosocomial transmission of MDRB. © 2014, Springer Science+Business Media New York.