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Lan F.,Tianjin Hospital | Pan Q.,Laiwu City Peoples Hospital | Yu H.,Peking University | Yue X.,Tianjin Neurosurgery Institute Tianjin
Journal of Neurochemistry | Year: 2015

Temozolomide (TMZ) has been widely used in the treatment of glioblastoma (GBM), although inherent or acquired resistance restricts the application. This study was aimed to evaluate the efficacy of sulforaphane (SFN) to TMZ-induced apoptosis in GBM cells and the potential mechanism. Biochemical assays and subcutaneous tumor establishment were used to characterize the function of SFN in TMZ-induced apoptosis. Our results revealed that β-catenin and miR-21 were concordantly expressed in GBM cell lines, and SFN significantly reduced miR-21 expression through inhibiting the Wnt/β-catenin/TCF4 pathway. Furthermore, down-regulation of miR-21 enhanced the pro-apoptotic efficacy of TMZ in GBM cells. Finally, we observed that SFN strengthened TMZ-mediated apoptosis in a miR-21-dependent manner. In conclusion, SFN effectively enhances TMZ-induced apoptosis by inhibiting miR-21 via Wnt/β-catenin signaling in GBM cells. These findings support the use of SFN for potential therapeutic approach to overcome TMZ resistance in GBM treatment. © 2015 International Society for Neurochemistry. Source

Li F.,Shandong University | Gao J.,Shandong University | Sokolove J.,Stanford University | Xu J.,Laiwu City Peoples Hospital | And 3 more authors.
International Journal of Immunogenetics | Year: 2014

Recent advances have highlighted a major genetic contribution to the pathogenesis of rheumatoid arthritis (RA).The aim of this study was to investigate whether polymorphisms of TNF-α (rs1800630, rs1800629) and TNFR1 (rs767455) were associated with susceptibility to and clinical outcome of RA in Chinese Han population. The target gene polymorphisms were genotyped in 256 patients with RA and 331 healthy controls using a high resolution melting (HRM) method. ESR, CRP, RF anti-CCP and anti-GPI level were also assayed and compared in genotypes of each polymorphism. Significant difference was observed in the genotype distributions and allele frequencies of TNF-α rs1800629 (P = 0.001, P < 0.001, respectively) between patients with RA and controls. There is no evidence to suggest an association between genotypes of the 3 SNPs according to age, gender, disease duration, DAS28 and serum level of autoantibodies. This study identifies a potentially important role for TNF-α rs1800629 polymorphisms in the susceptibility to RA.However, further studies in larger cohorts are required. © 2014 John Wiley & Sons Ltd. Source

Li F.,Shandong University | Xu J.,Laiwu City Peoples Hospital | Zheng J.,Shandong University | Sokolove J.,Stanford University | And 6 more authors.
Scientific Reports | Year: 2014

The aim of this study was to investigate the possible association in the interleukin-6 (IL-6) gene with Rheumatoid arthritis (RA) in Chinese Han population from Shandong Province. Target regions of IL-6 gene were amplified by polymerase chain reaction (PCR) and genotyped. A logistic regression analysis was performed to detect potential associations in our case-control sample, the odd ratio(OR) and 95% confidence intervals(CIs) were calculated. Furthermore, we systematically tracked all the published studies in the field and performed a meta-analysis for the single nucleotide polymorphisms (SNPs) under study. 256 RA patients and 331 healthy controls were recruited into the case-control study. We found allele frequencies of rs1800795, rs1800797 and rs1474347 in RA patients differ from control subjects (P = 0.016, 0.024, 0.020, respectively). Significant difference was observed in haplotype frequencies of GCCGCT between RA patients and controls (P = 0.0001, OR = 4.066, 95%CI = 1.891 ∼ 8.746), while GGCGCT frequencies was found lower in RA than controls (P = 0.006, OR = 0.669, 95%CI = 0.501 ∼ 0.894). The results of the meta-analysis showed association polymorphism within the IL-6 promoter with RA. These findings suggest that rare IL-6 gene polymorphisms may associate with RA susceptibility in Han Chinese populations; however further studies are needed to assess the validity of the association of IL-6 with RA. Source

Di H.,Laiwu City Peoples Hospital | Wu H.,Laiwu City Peoples Hospital | Gao Y.,Laiwu City Peoples Hospital | Li W.,Laiwu City Peoples Hospital | And 2 more authors.
Drug Development and Industrial Pharmacy | Year: 2016

Context: Combination anticancer therapy is promising to generate synergistic anticancer effects, to maximize the treatment effect and to overcome multi-drug resistance. Nanostructured lipid carriers (NLCs), composed of solid and liquid lipids, and surfactants are potentially good colloidal drug carriers. Objective: The aim of this study is to construct novel NLCs as nanocarriers for co-delivery of doxorubicin (DOX) and cisplatin (CDDP) to treat breast cancer. Methods: DOX and CDDP loaded NLCs (D–C-NLCs) were prepared by the solvent diffusion method. The in vitro cytotoxicity and synergistic studies of different formulations were evaluated on human breast cancer cells (doxorubicin resistant) (MCF-7/ADR cells). In vivo anti-tumor effects were observed on the murine bearing MCF-7/ADR cells model. Results: D–C-NLCs showed the highest cytotoxicity and synergistic effect of two drugs in tumor cells in vitro. The in vivo study revealed the greatest anti-tumor activity than the other formulations in the breast cancer model. Conclusion: The constructed NLCs could be used as a novel carrier for co-delivery of DOX and CDDP for breast cancer therapy. D–C-NLCs could be a promising targeted and combinational therapy nanomedicine. © 2016 Informa UK Limited, trading as Taylor & Francis Group Source

Lu X.,Shandong University | Si F.,Laiwu City Peoples Hospital | Zhang S.,Laiwu City Peoples Hospital | Liu Z.,Laiwu City Peoples Hospital
International Journal of Clinical and Experimental Pathology | Year: 2016

It is reported that miR-146a is associated with various tumors. However, its specific effect on glioma has not yet been studied. This paper is to explore the effect of miR-146a on glioma. RT-PCR method was used to detect the expression of miR-146a in neurogliocytoma tissue and corresponding non-tumor normal tissue, as well as in neurogliocytoma cell strain and neurogliocyte in normal people. IHC was used to detect the expression of MIF in neurogliocytoma tissue and corresponding non-tumor normal tissue. Relationship between MIF and miR-146a was detected with the luciferase reporter gene. Cell jamming technology was adopted, by which, miR-146a mimics and miR-146a negative control (NC) were transferred into cell line of neurogliocytoma for detecting the expressions of MIF mRNA and proteins. MTT method was used to detect cell viability and cloning experiments to detect the proliferative potential of cells. The expression quantity of miR-146a in neurogliocytoma tissue was lower than that in corresponding non-tumor normal tissue. The miR-146a in cell line of neurogliocytoma was lower than that in normal neuroglia tissue and the expression quantity in U87 was appropriate. The expression of MIF proteins in neurogliocytoma tissue was higher than that in corresponding non-tumor normal tissue. And luciferase reporter gene had verified that MIF was the downstream target gene of miR-146a and transferred to U87 cell by liposome. miR-146a shows low expressions in neurogliocytoma tissue and cell line, while MIF shows over expressions in neurogliocytoma tissue. The miR-146a expression can be up-regulated by targeted inhibiting the MIF expression, accordingly, to inhibit the proliferation of neurogliocytoma. Source

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