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Lahore, Pakistan

Hussain T.,The University of Lahore | Babar M.E.,The University of Lahore | Musthafa M.M.,University of Malaya | Saif R.,The University of Lahore | And 8 more authors.
Journal of Animal and Plant Sciences | Year: 2015

The taxonomy of wild sheep has been a complex debate with different classifications and revisions suggested based on their morphology, geographical distribution and chromosome number. In Pakistan the Punjab Urial (O. vigneipunjabiensis), is an important sub-species of O. vignei, however a scarce molecular data is reported that urged us to investigate its genetic diversity and phylogeny using mitochondrial DNA, ATPase6 and ATPase8 genes. A total of 842 bp complete coding region of both genes were amplified by PCR followed by sequencing. The sequences were aligned and edited using CodonCode Aligner and single nucleotide polymorphisms (SNPs) were identified. The boot strapped Neighbor joining tree constructed from MEGA6.1 explained the genetic relationships of O. v. punjabiensisis with Ovisammon (Argali) than the Ovisaries (sheep). The phylogenetic analysis also showed the genetic positioning of Punjab Urial with respect to other 23 different reported mammalian species as well. The study gave us useful genomic information about genetic diversity in Punjab Urial and its phylogenetic relationships with related taxa, emphasizing on need of executionof conservation strategies to protect this unique genetic resource of Pakistan. © 2015, Pakistan Agricultural Scientists Forum.All rights reserved.


Shahzad S.,Lahore Zoo | Saleem M.B.,Lahore Zoo | Mahmood A.K.,University of Veterinary and Animal Sciences | Shelly S.Y.,Lahore College for Women University | And 3 more authors.
Journal of Animal and Plant Sciences | Year: 2015

Conjunctivitis is an inflammation of the conjunctiva, often called pink eye and is common in young felids wild, captive or domestic. Two, 2½ months old puma cubs were reported with conjunctivitis at Lahore Zoo. They appeared dull and sluggish with protruded third eyelids along with ocular discharge and slight corneal opacity in both eyes. The cubs were treated with antibiotics and supplemented with vitamin A. Cubs responded the treatment within three days and recovered completely in four weeks. © 2015, Pakistan Agricultural Scientists Forum. All rights reserved.


Shahzad S.,Lahore Zoo | Tipu M.Y.,University of Veterinary and Animal Sciences | Aslam A.,University of Veterinary and Animal Sciences | Hussain T.,The University of Lahore | And 6 more authors.
Journal of Animal and Plant Sciences | Year: 2015

Theileria is a tick-borne protozoan infesting wild and domestic ruminants globally. This research was conducted to evaluate hemato-biochemical alterations in theileria infected Punjab Urial and domestic sheep and to study comparative efficiency of traditional screening methods with modern techniques. A total of 98 blood samples with thin blood smears of Urial and Domestic Sheep from Punjab, Pakistan with history of tick infestation, were collected and screened through microscopic techniques and PCR for Theileriosis respectively during summer 2013. Hematological parameters were analyzed by automatic hematology analyzer (Diatron Abacus) and Serum was analyzed by Semi-automatic Chemistry Analyzer (Micro Lab 300). Hematobiochemical findings revealed significant decrease (P<0.05) in Erythrocyte count, Leukocyte count, Hemoglobin Concentration, Pack Cell Volume, Total Proteins, Albumin and Creatinine in infected animals of both Urial and domestic sheep as compared with their control, while significant increase (P<0.05) in ALT, AST and Total Bilirubin was seen. Microscopic examination detected 47.6% of Urial and 35.6% of domestic sheep positive for Theileria while PCR analysis detected 54.7% positive samples in Urial and 51.7% in domestic sheep. This study concluded the basic data for values of hematology and serum biochemistry in Urial and domestic sheep with a higher susceptibility of Urial towards Theileriosis. © 2015 Pakistan Agricultural Scientists Forum. All rights reserved.

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