Hope B.C.,King's College |
Ameratunga R.,Auckland Hospital and Labplus |
Austin P.M.,Labplus |
Evans H.M.,Starship Childrens Hospital |
And 3 more authors.
Journal of Pediatric Gastroenterology and Nutrition | Year: 2013
OBJECTIVE: The aim of the present study was to evaluate a panel of different antibody assays, including second-generation antigliadin kits, in a local paediatric population thought to be at risk for coeliac disease (CD). METHODS: Seventy-nine children, who tested positive for immunoglobulin A (IgA) antibodies to tissue transglutaminase (TG), underwent duodenal biopsy. At endoscopy, serum was collected from all of the patients, and 9 different coeliac antibody assays were performed, both as isolated assays and in combination. These included immunoglobulin A (IgA) anti-tissue transglutaminase (TGA), and IgA plus IgG anti-deamidated gliadin peptide (DGPAG). A diagnosis of CD was made if the biopsies showed Marsh grade 3 lesions. RESULTS: Twenty-four of 79 children had CD confirmed histologically. Only 39 of 79 were positive for Inova TGA, and 35 of 79 were positive for Inova DGPAG. Twenty-four of 39 who were TGA positive and 24 of 35 who were DGPAG positive had confirmed CD on biopsy. There was good correlation between TGA and DGPAG-positive predictive values. None of the modified gliadin tests produced false-negative results, and neither did the TGA. CONCLUSIONS: The Inova DGPAG and TGA assays have similar use in predicting CD in a selected paediatric population; however, in children who are positive for TGA when screened for CD, more than half have negative TGA serology when repeat testing is done at the time of biopsy. Those with persistent TGA positivity have only a 61.5% probability of having histologic CD, compared with 68.6% of those children positive for DGPAG. Copyright © 2013 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition.
Buckley E.,Auckland City Hospital |
Sidebotham D.,Auckland City Hospital |
McGeorge A.,Auckland City Hospital |
Roberts S.,Labplus |
And 3 more authors.
British Journal of Anaesthesia | Year: 2010
We report four patients with pandemic H1N1 2009 influenza virus and secondary bacterial infection who were treated with extracorporeal membrane oxygenation (ECMO) for cardiorespiratory failure. Three of the four patients had profound shock, necessitating support with venoarterial ECMO. Two patients died during ECMO support. The two survivors had prolonged hospital stays, which were complicated by renal failure and limb ischaemia.
Siebers R.,University of Otago |
Siebers R.,New Zealand Institute of Medical Laboratory Science |
New Zealand Journal of Medical Laboratory Science | Year: 2011
The New Zealand Institute of Medical Laboratory Science (NZIMLS) is the organisation that represents those engaged in the profession of Medical Laboratory Science in New Zealand. Its role is to promote professional excellence through communication, education and a code of ethics to achieve the best laboratory service for the benefit of the patient. As such it provides a variety of services for its members. In 2006 a members survey was conducted to determine how well the NZIMLS, and services it provided, performed. Results from that survey identified various areas of concern. Five years on the survey was repeated. A web-based questionnaire was filled in by 379 members, a response rate of 19.7% of the total membership (n=2,013). Top services and activities rated by the members were the journal, Council newsletter, CPD programme and the Executive Office; while the web site and promotion of the profession were rated low. Top activities of importance to members were Council governance, web site, CPD programme and QMLT/QSST examinations. Results from the survey will be valuable to the NZIMLS Council in improving its services to its members.
New Zealand Journal of Medical Laboratory Science | Year: 2010
Objective: The distinction between benign mesothelial cells and malignant mesothelial cells in fl uid cytology is a common scenario facing pathologists. This study aims to examine the usefulness of Glut-1 as a tool in making this distinction when applied to cell block material. Glut-1 staining was interpreted in conjunction with desmin. Glut-1 staining in mesothelioma was compared to epithelial membrane antigen staining. Methods: 34 cytology cases (17 benign and 17 mesothelioma) reported at Labplus between 2002 and 2009 were reviewed. Glut-1, desmin and epithelial membrane antigen immunohistochemistry were applied to the cell blocks. All cases in both categories had the diagnoses confi rmed histologically. The staining results were then divided into negative, focal positive (<30% staining) and diffuse positive (>30% staining). Results: Glut-1 positivity was seen in 82.4% of malignant mesotheliomas. Only one case of mesothelioma showed focal weak positivity for desmin while the other 16 cases were completely negative. All 17 cases of benign mesothelial proliferation were negative for Glut-1. Of the 17 cases of benign mesothelial proliferation, two showed focal positivity for desmin, four were negative, while the rest were all strongly positive. Conclusion: Glut-1 immunohistochemistry was proven to be a useful adjunct to routine fl uid cytology in patients with possible mesothelioma. Negative staining with Glut-1 does not rule out mesothelioma, especially when clinical suspicion is high. Glut-1 is best interpreted together with other markers including epithelial membrane antigen and desmin.
Comparison of two methods for the diagnosis of chronic granulomatous disease - neutrophil oxidative burst measured by the nitroblue tetrazolium slide test versus the dihydrorhodamine 123 flow cytometric assay
Dimitrova G.,LabPlus |
Bunkall C.,LabPlus |
Lim D.,LabPlus |
Kendrick C.,Massey University
New Zealand Journal of Medical Laboratory Science | Year: 2013
Objective: The neutrophil respiratory burst is crucial for the ability of the host to kill ingested microorganisms. The detection of this activity is an essential part of the laboratory investigation of patients with suspected chronic granulomatous disease (CGD). In this study the traditional qualitative nitroblue tetrazolium (NBT) slide test was compared with a quantitative whole blood dihydrorhodamine 123 (DHR 123) assay using flow cytometry. Methods: A total of 20 samples submitted to Labplus at Auckland Hospital were screened for CGD by both the NBT and the DHR 123 assays. Results: While the NBT method was able to demonstrate reduced NADPH oxidase in CGD patients, it is highly subjective and cannot identify carrier states of X-linked-type CGD. In contrast, the quantitative whole blood dihydrorhodamine 123 (DHR 123) flow cytometric method evaluated in this study was more rapid, allowing the proportion of affected cells to be determined, and was able to identify the carrier state of X-linked CGD. Conclusions: The DHR 123 assay proved to be a more sensitive and more convenient method for the measurement of neutrophil oxidative burst activity and showed a number of advantages over the qualitative NBT slide test for the diagnosis of CGD. © 2013 The authors.