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Kaiser J.,Brno University of Technology | Kaiser J.,Elettra - Sincrotrone Trieste | Hola M.,Masaryk University | Galiova M.,Brno University of Technology | And 10 more authors.
Urological Research | Year: 2011

The outcomes from the feasibility study on utilization of synchrotron radiation X-ray microtomography (SR-μCT) to investigate the texture and the quantitative mineralogical composition of selected calcium oxalate-based urinary calculi fragments are presented. The comparison of the results obtained by SR-μCT analysis with those derived from current standard analytical approaches is provided. SR-μCT is proved as a potential effective technique for determination of texture, 3D microstructure, and composition of kidney stones. © 2010 Springer-Verlag. Source


Kuta J.,Masaryk University | MacHat J.,Masaryk University | Benova D.,Masaryk University | Cervenka R.,Masaryk University | Koristkova T.,Laboratory Specializing in Urinary Stones Analyses
Central European Journal of Chemistry | Year: 2012

Chemical analysis of various biological matrices is routinely used for assessment of human exposure to various toxic metals. In this work, 489 samples of urinary calculi originating from almost the whole of The Czech Republic, were collected and subjected to mineralogical and elemental analysis. This study was aimed at mercury, the content of which was determined using thermo-oxidation - cold vapor - atomic absorption spectrometry. The effects of mineralogical composition, sex, age and region were recorded in order to verify the applicability of urinary calculi for biomonitoring. Relationships with other minor and trace elements were also investigated. Association of mercury with whewellite mineral was observed as well as a remarkable relationship with selenium, confirming the role of selenium in mercury excretion. No statistically significant effect was observed on the mercury content in stones with regard to the sex or region. Median values in age groups follow a trend with the maximum median value 0.365 mg kg -1 in the group of 41 - 50 year old donors, decreasing to 0.060 mg kg -1 for the oldest group (81 - 92 years). Our results confirm that urinary calculi can be helpful in providing complementary information on human exposure to mercury and its excretion. © Versita Sp. z o.o. Source


Kuta J.,Masaryk University | Smetanova S.,Masaryk University | Benova D.,Masaryk University | Koristkova T.,Laboratory Specializing in Urinary Stones Analyses | Machat J.,Masaryk University
Environmental Geochemistry and Health | Year: 2016

Monitoring of body burden of toxic elements is usually based on analysis of concentration of particular elements in blood, urine and/or hair. Analysis of these matrices, however, predominantly reflects short- or medium-term exposure to trace elements or pollutants. In this work, urinary stones were investigated as a matrix for monitoring long-term exposure to toxic and essential elements. A total of 431 samples of urinary calculi were subjected to mineralogical and elemental analysis by infrared spectroscopy and inductively coupled plasma mass spectrometry. The effect of mineralogical composition of the stones and other parameters such as sex, age and geographical location on contents of trace and minor elements is presented. Our results demonstrate the applicability of such approach and confirm that the analysis of urinary calculi can be helpful in providing complementary information on human exposure to trace metals and their excretion. Analysis of whewellite stones (calcium oxalate monohydrate) with content of phosphorus <0.6 % has been proved to be a promising tool for biomonitoring of trace and minor elements. © 2015, Springer Science+Business Media Dordrecht. Source

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