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Piccione G.,Laboratory of Veterinary Chronophysiology | Giannetto C.,Laboratory of Veterinary Chronophysiology | Faggio C.,Messina University | Alberghina D.,Laboratory of Veterinary Chronophysiology | Panzera M.,Laboratory of Veterinary Chronophysiology
Biological Rhythm Research

The aim of the present study was to investigate the influence of three-time feeding on insulin daily rhythm in horses. At this purpose, five clinically healthy Italian saddle horses were housed under natural photoperiod (L/D) followed by constant darkness (D/D). Horses were fed three times a day (6:00; 12:00; 18:00) with hay and a mix of cereals. Blood samples were collected every 3 h over 48-h period (24 h during L/D and 24 h during D/D). On each serum sample insulin concentration was assessed. Two-way for repeated measures analysis of variance (ANOVA) was applied to investigate statistically differences due to time of day and experimental conditions. Cosinor rhythmometry was applied to investigate daily rhythmicity in the two experimental periods. Our results showed a significant effect of time of day and experimental conditions on serum insulin concentrations. Daily rhythmicity of insulin serum concentrations was observed during L/D and D/D. Statistically differences in MESOR (Midline-Estimating Statistic of Rhythm), amplitude and acrophases were observed between L/D and D/D. MESOR and amplitude statistically decreased in D/D respect to L/D, and acrophase was postponed in L/D respect to D/D. Robustness of rhythm did not change between the two experimental period. In conclusion, we can hypothesised that the daily rhythm of insulin in horses is endogenously generated; and that during the routine management of the stabled horses providing feed three times a day favourite the physiological secretion of insulin in this species. © 2013 Copyright Taylor and Francis Group, LLC. Source

D'Alterio G.,University of Padua | Casella S.,Laboratory of Veterinary Chronophysiology | Gatto M.,University of Padua | Gianesella M.,University of Padua | And 2 more authors.
Czech Journal of Animal Science

The circadian rhythm of body core and surface temperature in 10 Comisana sheep kept under a natural photoperiod (06:30/19:00) was evaluated. Every 3 h for 24 consecutive hours rectal temperature (RT) and foot temperature were recorded. Particularly, foot temperature was recorded by infrared thermography, an ideal technique for evaluating the temperature not only at one point but also at the eight points as follows: in the right front of the interdigital area (FA1), in the left front of the interdigital area (FA2), in the right rear of the interdigital area (RA1), in the left rear of the interdigital area (RA2), in the right front of the interdigital line (FL1), in the left front of the interdigital line (FL2), in the right rear of the interdigital line (RL1) and in the left rear of the interdigital line (RL2). Two-way repeated measures ANOVA using SPSS, followed by Scheffé's test, showed a significant effect of the time of day and side of temperature collection (P < 0.05) on temperature values. The single cosinor procedure showed a daily rhythmicity of RT and foot temperature in all tested sides. The circadian oscillations of RT reflected the familiar circadian patterns of endogenous sources and the results of foot temperatures supported the idea that the daily rhythm was, at least in part, influenced by variation in the blood flow to the extremities. The infrared thermography providing more information on the development of disturbances in the peripheral circulation may be used with an advantage in occupational health examinations and in special clinical work. Source

Fazio F.,Laboratory of Veterinary Chronophysiology | Faggio C.,Messina University | Marafioti S.,Laboratory of Veterinary Chronophysiology | Torre A.,Messina University | And 2 more authors.
Cahiers de Biologie Marine

The present study is aimed to assess the influence of different habitats, Faro Lake and Tyrrhenian Sea, on the haematological profile of Gobius niger. For both sites of collection water chemical-physical parameters were measured. Blood samples were taken from 20 fish captured in Faro Lake (group A) and from 20 fish captured in Tyrrhenian Sea (group B) to measure haematological profile that included Red Blood Cell Count (RBC), Hematocrit (Hct), Hemoglobin concentration (Hgb), White Blood Cell Count (WBC), Thrombocyte Count (TC), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Red cell Distribution Width (RDW), Mean Thrombocyte Volume (MTV), Thrombocyte Distribution Width (TDW) and Thrombocytocrit (Tct). Results showed significantly statistical differences in RBC, Hct, MCH and MCHC between two different locations that are related to variations of the chemical and physical parameters of the water of two different sites of collection. Our findings emphasize the fact that changes in blood characteristics are important indices in monitoring the effects of habitat changes on the physiology of G. niger. Source

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