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Riondino S.,Laboratory of Thrombosis and Haemostasis | La Farina F.,Laboratory of Thrombosis and Haemostasis | Martini F.,Laboratory of Thrombosis and Haemostasis | Guadagni F.,Interinstitutional Multidisciplinary BioBank BioBIM | Ferroni P.,Laboratory of Thrombosis and Haemostasis
International Archives of Occupational and Environmental Health | Year: 2011

Purpose Progressive functional impairments develop with chronic repetitive tasks possibly involving inflammatory mediators. Aim of this study was to analyze systemic inflammatory changes in relation to the possible occurrence of pain and/or disability in video terminal operators (VTOs) undergoing upper-extremity repetitive stress due to chronic overuse. Methods Pain assessments, classification, and grade of impairment relied on self-report questionnaires administered to 21 VTOs and to 21 matched controls. The inflammatory status of the enrolled subjects was analyzed by determination of serum high sensitive C-reactive protein (hs-CRP) as well as systemic levels or monocyte expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Results Serum levels of both cytokines were increased in VTOs compared to controls (P = 0.005 for TNF-α and P = 0.004 for IL-6). TNF-α levels correlated to IL-6 (P = 0.019), which, in turn, was associated to increased hs-CRP (P = 0.012). DASH score allowed to categorize VTOs according to disability. VTOs with mild (DASH = 22) or moderate (DASH = 46) disability (n = 10) had higher serum hs-CRP (P = 0.001) and IL-6 (P = 0.035) levels than VTOs without disabilities (DASH < 17) (n - 11). Monocyte stimulatory TNF-α expression was increased in individuals with mild/moderate disability. Monocyte expression of TNF-α was independently associated to that of IL-6, which, in turn, was associated to increased systemic hs-CRP levels together with mild/moderate functional impairment and weekly commitment to the display screen. Conclusions The results here reported indicate the occurrence of a low-grade inflammatory condition in VTOs with mild/moderate disability, which might allow the early recognition of arising musculoskeletal disorders induced by repetitive stress. © Springer-Verlag 2010.

Riondino S.,Laboratory of Thrombosis and Haemostasis | Martini F.,Laboratory of Thrombosis and Haemostasis | La Farina F.,Laboratory of Thrombosis and Haemostasis | Spila A.,Interinstitutional Multidisciplinary BioBank BioBIM | And 2 more authors.
Clinical Biochemistry | Year: 2010

Objectives: To investigate whether sCD40L dosage might represent a useful tool to explore in vivo platelet function. Design and methods: sCD40L and sP-selectin levels and light transmission aggregometry (LTA) were analyzed in 69 healthy donors. Immunoassays were performed on platelet-depleted citrate plasma samples. The effects of in vitro aspirin treatment on the release of sCD40L were investigated in 15 subjects following platelet stimulation. The effects of a 1-month therapeutic course of low-dose aspirin on sP-selectin and sCD40L levels were also investigated. Results: A significant correlation was observed between sCD40L and sP-selectin (p<0.01). In vitro aspirin treatment remarkably decreased sCD40L levels following platelet activation by exogenous agonists. sCD40L directly correlated with LTA (Rho = 0.62, p<0.0001). In vivo aspirin treatment significantly reduced both sP-selectin and sCD40L levels (both p<0.01) in a direct correlation (Rho = 0.66, p<0.05). Conclusions: Citrated plasma samples reflect sCD40L released from platelets, thus yielding the most valid estimates of in vivo circulating levels of this platelet activation markers. © 2010 The Canadian Society of Clinical Chemists.

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