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Guangzhou, China

Liu S.,Laboratory of Surgery | Zeng Y.,Sun Yat Sen University | Li Y.,Laboratory of Surgery | Guo W.,Laboratory of Surgery | And 2 more authors.
Tumor Biology | Year: 2014

Vasoactive intestinal peptide (VIP) is a neurotransmitter that primarily functions as a vasodilator. VIP plays its role through binding to its receptors known as VIP/pituitary adenylate cyclase-activating peptide receptors (VPACs). In this study, we examined the expression of VPAC1 in human colon cancer tissues, analyzed the relationship between VPAC1 expression and cancer malignancy, and explored the possible mechanisms using immunohistochemistry and immunofluorescence double staining. The results showed that (1) poorly differentiated colon cancers have significantly higher VPAC1 expression than well-differentiated colon cancers do (p<0.01); (2) phospho-epithelial growth factor receptor (EGFR) overexpression/activation in the cytoplasm of cancer cells is related to VPAC1 overexpression; (3) blood vessels surrounding colon cancer have significantly more VPAC1-positive than normal colon mucosa does; (4) tumor-associated macrophages (TAMs) of colon cancer have a higher level of VPAC1 expression than macrophages in normal colon mucosa do. These data suggest that VPAC1 overexpression is associated with poorer differentiation of colon cancer, which is likely caused by subsequent EGFR activation in cancer cells. In addition, VPAC1 overexpression in both blood vessels and macrophages in tumors may also play an important role in the development of aggressive cancer. © 2014 International Society of Oncology and BioMarkers (ISOBM). Source

Zheng Q.,Laboratory of Surgery | Gong F.,Laboratory of Surgery | Xu Y.,Laboratory of Surgery | Zheng T.,Laboratory of Molecular Pathology | Ying M.,Fujian Provincial Tumor Hospital
Tumori | Year: 2011

Aims. To obtain floating spheres from the adherent gastric cancer cell line SGC-7901 and to analyze the properties of the spheres. Methods. Serum-free medium was applied to cultured SGC-7901 cells. Limiting dilution assay, tumor formation assay, microarray analysis, and real-time fluorescent quantitative PCR were used to test the stem cell properties of the spheres. Results. A subpopulation of SGC-7901 cells formed floating spheres in serum-free medium. These cells showed enhanced tumorigenic ability and also highly expressed certain stem-cell-associated proteins. Conclusions.We successfully propagated floating spheres with stemcell properties in the SGC-7901 gastric cell line. Source

Hong C.,Laboratory of Surgery | Wei Y.,Laboratory of Surgery | Jiang J.,Affiliated Hospital of Guiyang Medical College | Zhao C.,Laboratory of Surgery | And 3 more authors.
Asia-Pacific Journal of Clinical Oncology | Year: 2014

Aims: To explore the etiology of the neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) abnormalities in colorectal cancer. Methods: In total, 230 patients with histopathologically confirmed colorectal cancer from August 2009 to August 2011 were recruited to our study. The associations between lifestyles (smoking, alcohol and pickled food consumption) and pretreatment NLR and PLR were estimated using the Kruskal-Wallis tests and linear regression model. Results: The Kruskal-Wallis test showed a significant association between pickled food intake and pretreatment NLR but not PLR (P=0.002, 0.057, respectively). Pairwise comparisons showed that, compared with those with a moderately frequent (2-3 times/week) and an infrequent (≤once a week) intake of pickled food, high frequency (≥ four times/week) consumption of pickled food had a higher pretreatment NLR (P=0.01, 0.007, respectively). Multivariate linear regression analysis showed pretreatment NLR increased significantly in high frequency (≥ four times/week) consumption of pickled food (P<0.0001). No association between other lifestyle factors and pretreatment PLR was found. Conclusions: A higher frequency intake of pickled food possibly contributes to higher NLR, which may reflect a systemic inflammatory response in colorectal cancer. © 2013 Wiley Publishing Asia Pty Ltd. Source

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