Li J.,Laboratory of Stem Cells and Tissue Engineering |
Wei Q.,Laboratory of Stem Cells and Tissue Engineering |
Zuo G.-W.,Key Laboratories of Clinical Diagnostics |
Xia J.,Laboratory of Stem Cells and Tissue Engineering |
And 3 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014
Ginsenoside Rg1 is one effective anticancer and antioxidant constituent of total saponins of Panax ginseng (TSPG), which has been shown to have various pharmacological effects. Our previous study demonstrated that Rg1 had anti-tumor activity in K562 leukemia cells. The aim of this study was designed to investigate whether Rg1 could induce apoptosis in TF-1/Epo cells and further to explore the underlying molecular mechanisms. Here we found that Rg1 could inhibit TF-1/Epo cell proliferation and induce cell apoptosis in vitro in a concentration and time dependent manner. It also suppressed the expression of EpoR on the surface membrane and inhibited JAK2/STAT5 pathway activity. Rg1 induced up-regulation of Bax, cleaved caspase-3 and C-PAPR protein and down-regulation of Bcl-2 and AG490, a JAK2 specific inhibitor, could enhance the effects of Rg1. Our studies showed that EpoR-mediated JAK2/STAT5 signaling played a key role in Rg1-induced apoptosis in TF-1/Epo cells. These results may provide new insights of Rg1 protective roles in the prevention a nd treatment of leukemia.
Xu Y.-H.,Laboratory of Stem Cells and Tissue Engineering |
Li M.,Laboratory of Stem Cells and Tissue Engineering |
Liu J.,Laboratory of Stem Cells and Tissue Engineering |
Jiang R.,Laboratory of Stem Cells and Tissue Engineering |
Wang W.-W.,Laboratory of Stem Cells and Tissue Engineering
Acta Anatomica Sinica | Year: 2011
Objective: To observe the effect of transplanted bone marrow mesenchymal stem cells (MSCs) on repairing of the colon tissues of rats with ulcerative colitis. Methods: The MSCs from rats were isolated, cultured and labelled with fluor. Forty-five SD Rats were randomly grouped as A, B and C groups. Immune-combined TNBS/ethanol was induced in groups A and B. Group C was the control group. The rat in group A received a caudal vein injection of 1ml of a fluids containing the labelled MSCs, and the rat in group B received 1ml of PBS after the induction of colitis. 5 rats in each group were sacrificed at day 3, 7 and 14 after injection. The colonic tissue in 3 groups were collected and prepared as paraffin sections for examination of pathological changes. Some colonic tissues in group A were prepared as frozen sections to observe the distribution of transplanted cells in the colon under a fluorescent microscope, and expression of cytokeratin (CK) of transplanted cells by immunofluorescent staining under a laser confocal microscope. Results: The MSCs proliferated rapidly and had a typical fibroblast-like morphology. The repairing of injured colon in group A was much better than that in group B on day 7 and 14. The transplanted cells were seen in colon in group A, and the number of the cells increased as time going and was maximized on day 14 (P < 0.05). There were a few transplanted cells expressed CK in group A on day 7 and 14. Conclusion: The transplanted MSCs distribute in the injured colon of rats with ulcerative colitis, and some MSCs diferentiate into colonic epithelial cells and participate in repair of the injured colon.