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Ymittos Athens, Greece

Raggi C.,U.S. National Institutes of Health | Berardi A.C.,Laboratory of Stem Cells
Muscles, Ligaments and Tendons Journal

Tissue maintenance and regeneration is dependent on stem cells and increasing evidence has shown to decline with age. Stem cell based-aging is thought to influence therapeutic efficacy. Mesenchymal stromal cells (MSCs) are involved in tissue regeneration. Here, we discuss the effects of age-related changes on MSC properties considering their possible use in research or regenerative medicine. © CIC Edizioni Internazionali. Source

Berardi A.C.,Laboratory of Stem Cells
Recent Patents on Drug Delivery and Formulation

The in vitro generation of hematopoietic stem cells (HSCs) and mature hematopoietic cells from hemangioblast derived from embryonic stem (ES) or induced pluripotent stem (iPS) cells promises to provide an alternative source of cells that could replace total bone marrow cells or HSC-enriched fractions. This mini-review deals with innovation related to hemangioblast-based methods for blood cells production as disclosed in recent patent literature and current barriers to clinical translation are discussed. © 2013 Bentham Science Publishers. Source

Osti L.,Unit of Arthoscopy and Sports Trauma Surgery | Berardocco M.,Laboratory of Stem Cells | Di Giacomo V.,University of Chieti Pescara | Di Bernardo G.,U.O.C. of Immunohaematology and Transfusion Medicine | And 2 more authors.
BMC Musculoskeletal Disorders

Background: Hyaluronic Acid (HA) has been already approved by Food and Drug Administration (FDA) for osteoarthritis (OA), while its use in the treatment of tendinopathy is still debated. The aim of this study was to evaluate in human rotator cuff tendon derived cells the effects of four different HA on cell viability, proliferation, apoptosis and the expression of collagen type I and collagen type III. Methods: An in vitro model was developed on human tendon derived cells from rotator cuff tears to study the effects of four different HA preparations (Ps) (sodium hyaluronate MW: 500-730 KDa - Hyalgan®, 1000 kDa Artrosulfur HA®, 1600 KDa Hyalubrix® and 2200 KDa Synolis-VA®) at various concentrations. Tendon derived cells morphology were evaluated after 0, 7 and 14 d of culture. Viability, proliferation, apoptosis were evaluated after 0, 24 and 48 h of culture. The expression and deposition of collagen type I and collagen type III were evaluated after 1, 7 and 14 d of culture. Results: All HAPs tested increased viability and proliferation, in dose dependent manner. HAPs already reduce apoptosis at 24 h compared to control cells (without HAPs). Furthermore, HAPs stimulated the synthesis of collagen type I in a dose dependent fashion over 14 d, without increase in collagen type III; moreover, in the presence of Synolis-VA® the expression and deposition of collagen type I was significantly higher as compare with the other HAPs. Conclusions: HAPs enhanced viability, proliferation and expression of collagen type I in tendon derived cells. © 2015 Osti et al. Source

Oliva F.,University of Rome Tor Vergata | Berardi A.C.,Laboratory of Stem Cells | Misiti S.,University of Rome La Sapienza | Maffulli N.,University of Salerno | Maffulli N.,Queen Mary, University of London
Muscles, Ligaments and Tendons Journal

Thyroid hormones (THs) T3 and T4, play an essential role in the development and metabolism of many tissues and organs, and have profound metabolic effects in adult life. THs action is mediated mainly by the thyroid hormone receptor (TRs) which seem to be ubiquitous. To-date thyroid-associated disease are not thought to be related in tendinopathies and tendons tears. Recent study demonstrated the presence of TRs in tendons and their possible role in the proliferation and apoptosis of human tenocyte isolated from tendon. We review new discovery that revisit our current thinking on the tendon biology focusing on thyroid hormones (THs) T3 and T4, and their possible role on human tenocyte. Source

Ciraci E.,Laboratory of Stem Cells | Della Bella S.,University of Milan | Salvucci O.,U.S. National Institutes of Health | Rofani C.,University of Rome La Sapienza | And 6 more authors.

A precise identification of adult human hemangioblast is still lacking. To identify circulating precursors having the developmental potential of the hemangioblast, we established a new ex vivo long-term culture model supporting the differentiation of both hematopoietic and endothelial cell lineages. We identified from peripheral blood a population lacking the expression of CD34, lineage markers, CD45 and CD133 (CD34-Lin-CD45 -CD133- cells), endowed with the ability to differentiate after a 6-week culture into both hematopoietic and endothelial lineages. The bilineage potential ofCD34-Lin-CD45-CD133 - cells was determined at the single-cell level in vitro and was confirmed by transplantation into NOD/SCID mice. In vivo,CD34 -Lin-CD45-CD133- cells showed the ability to reconstitute hematopoietic tissue and to generate functional endothelial cells that contribute to new vessel formation during tumor angiogenesis. Molecular characterization of CD34-Lin -CD45-CD133- cells unveiled a stem cell profile compatible with both hematopoietic and endothelial potentials, characterized by the expression of c-Kit and CXCR4 as well as EphB4, EphB2, and ephrinB2. Further molecular and functional characterization of CD34-Lin -CD45-CD133- cells will help dissect their physiologic role in blood and blood vessel maintenance and repair in adult life. Source

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