Laboratory of Research on Biologically Compatible Compounds

Monastir, Tunisia

Laboratory of Research on Biologically Compatible Compounds

Monastir, Tunisia

Time filter

Source Type

Chargui I.,University of Sfax | Grissa I.,University of Sfax | Bensassi F.,Laboratory of Research on Biologically Compatible Compounds | Bensassi F.,National Institute for Agricultural Research | And 4 more authors.
Biomedical and Environmental Sciences | Year: 2012

Objective: To evaluate histopathological alterations of the liver and kidney of female rats exposed to low doses of DM and its potential genotoxic activity. Methods: Female Wistar rats were randomly assigned to control (3 groups, 6 rats in each) and treatment groups (3 groups, 6 rats in each). They were subjected to subcutaneous injections of DM (at doses of 0.003, 0.03, and 0.3 mg/kg bw/d) after 30, 45, and 60 d, respectively. Results: Significant alterations were recorded in liver parenchyma induced by hepatic vacuolization, fragmented chromatin in nuclei, dilatation of sinusoids and congestions. Lesions within proximal and distal tubules were observed in the kidneys. Tissue congestions and severe alterations within glomeruli were visible. DM as a pyrethroid insecticide induced significant increase (P≤0.05) of plasma MDA concentrations after 45 d. A significant increase (P≤0.05) in plasma ALT (after 45 and 60 d) and AST (after 60 d) concentrations was recorded as compared to controls. During the whole experimental period the toxic agent provoked significant DNA damages (P≤0.05), especially in the dominance of classes 3 and 4 of obtained comet. Conclusion: DM even at a very low dose displays harmful effects by disrupting hepatic and renal function and causing DNA damages in puberscent female rats. Low doses of DM are hepatotoxic and nephrotoxic. © 2012 The Editorial Board of Biomedical and Environmental Sciences.


Brahmi D.,Laboratory of Research on Biologically Compatible Compounds | Brahmi D.,Research Unit of Macromolecular Biochemistry and Genetic | Bouaziz C.,Laboratory of Research on Biologically Compatible Compounds | Ayed Y.,Laboratory of Research on Biologically Compatible Compounds | And 4 more authors.
Nutrition and Metabolism | Year: 2011

Background: Aflatoxin B1 (AFB1) is potent hepatotoxic and hepatocarcinogenic agent. In aflatoxicosis, oxidative stress is a common mechanism contributing to initiation and progression of hepatic damage. The aim of this work was to evaluate the hepatoprotective effect of cactus cladode extract (CCE) on aflatoxin B1-induced liver damage in mice by measuring malondialdehyde (MDA) level, the protein carbonyls generation and the heat shock proteins Hsp 70 and Hsp 27 expressions in liver. We also looked for an eventual protective effect against AFB1-induced genotoxicity as determined by chromosome aberrations test, SOS Chromotest and DNA fragmentation assay. We further evaluated the modulation of p53, bax and bcl2 protein expressions in liver. Methods. Adult, healthy balbC (20-25 g) male mice were pre-treated by intraperitonial administration of CCE (50 mg/Kg.b.w) for 2 weeks. Control animals were treated 3 days a week for 4 weeks by intraperitonial administration of 250 g/Kg.b.w AFB1. Animals treated by AFB1 and CCE were divided into two groups: the first group was administrated CCE 2 hours before each treatment with AFB1 3 days a week for 4 weeks. The second group was administrated without pre-treatment with CCE but this extract was administrated 24 hours after each treatment with AFB1 3 days a week for 4 weeks. Results: Our results clearly showed that AFB1 induced significant alterations in oxidative stress markers. In addition, it has a genotoxic potential and it increased the expression of pro apoptotic proteins p53 and bax and decreased the expression of bcl2. The treatment of CCE before or after treatment with AFB1, showed (i) a total reduction of AFB1 induced oxidative damage markers, (ii) an anti-genotoxic effect resulting in an efficient prevention of chromosomal aberrations and DNA fragmentation compared to the group treated with AFB1 alone (iii) restriction of the effect of AFB1 by differential modulation of the expression of p53 which decreased as well as its associated genes such as bax and bcl2. Conclusion: We concluded that CCE might have a hepatoprotective effect against aflatoxicosis in mice, probably acting by promoting the antioxidant defence systems. © 2011Brahmi et al; licensee BioMed Central Ltd.


Rjiba-Touati K.,Laboratory of Research on Biologically Compatible Compounds | Ayed-Boussema I.,Laboratory of Research on Biologically Compatible Compounds | Skhiri H.,University of Monastir | Belarbia A.,University of Sousse | And 3 more authors.
Mutation Research - Genetic Toxicology and Environmental Mutagenesis | Year: 2012

Cisplatin (Cisp) is one of the most effective chemotherapeutic agents. However, at higher doses several side effects may occur. Recombinant human erythropoietin (rhEPO), a glycoprotein regulating haematopoiesis, has recently been shown to exert an important cyto-protective effects in many tissues. The purpose of this study was to explore whether rhEPO protects against Cisp-induced genotoxicity in rat bone-marrow cells. Adult male Wistar rats were divided into six groups of 18 animals each: control group, rhEPO-alone group, Cisp-alone group and three rhEPO+Cisp-groups (pre-, co- and post-treatment condition, respectively). Our results show that Cisp induced a noticeable genotoxic effect in rat bone-marrow cells. In all types of treatment, rhEPO significantly decreased the frequency of micronuclei, the percentage of chromosome aberrations and the level of DNA damage. The protective effect of rhEPO was more efficient when it was administrated 24. h before exposure to Cisp. © 2012 Elsevier B.V.


El Golli-Bennour E.,Laboratory of Research on Biologically Compatible Compounds | Bacha H.,Laboratory of Research on Biologically Compatible Compounds
Toxicology | Year: 2011

The environment represents a key contributor to human health and disease. Exposure to many substances such as pollutants, toxins and chemicals, has detrimental effects on health and are considered to contribute substantially to most diseases of major public health significance. Environmental diseases as mycotoxicosis are those in general aroused or exacerbated by exposure to environmental stressors as mycotoxins. These hazardous compounds are secondary metabolites produced by fungi and occurred simultaneously in food, feed and raw materials. The present investigation was conducted to assess if (i) Hsp70 induction, a parameter of protective and adaptive response, is a systematic biomarker to mycotoxin intoxications and (ii) all mycotoxins undergo oxidative stress in there toxic signalling pathways, as the omnipresent process playing a role in the initiation or progression of numerous disorders. Overall, observations to date evoke that Hsp70 can act as biomarkers of oxidative injury instead they are not systematic to mycotoxin exposure. © 2011 Elsevier Ireland Ltd.


El Golli-Bennour E.,Laboratory of Research on Biologically Compatible Compounds | Kouidhi B.,Laboratory of Research on Biologically Compatible Compounds | Bouslimi A.,Laboratory of Research on Biologically Compatible Compounds | Abid-Essefi S.,Laboratory of Research on Biologically Compatible Compounds | And 2 more authors.
Journal of Biochemical and Molecular Toxicology | Year: 2010

Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are important food-borne mycotoxins that have been implicated in human health. In this study, independent and combinative toxicities of AFB1 and OTA were tested in cultured monkey kidney Vero cells. The experiments reported here were conducted to evaluate the effect of these toxins on cell viability followed by the determination of cell death pathways, using the quantification of DNA fragmentation and the expression of p53 and bcl-2 protein levels. Our results showed that AFB1 and OTA caused a marked decrease of cell viability in a dose-dependent manner. Under the same conditions, these mycotoxins increased fragmented DNA levels. In addition, p53 was activated in response to DNA damage and the expression of the antiapoptotic factor bcl-2 decreased significantly. According to these data, AFB1 and OTA seemed to be involved in an apoptotic process. Moreover, combined AFB1 and OTA induced all the toxicities observed with the mycotoxins separately. Therefore, this combination was classified as an additive response of the two mycotoxins. © 2010 Wiley Periodicals, Inc.


Bouaziz C.,Laboratory of Research on Biologically Compatible Compounds | Bouslimi A.,Laboratory of Research on Biologically Compatible Compounds | Kadri R.,Laboratory of Research on Biologically Compatible Compounds | Zaied C.,Laboratory of Research on Biologically Compatible Compounds | And 2 more authors.
Experimental and Toxicologic Pathology | Year: 2013

The aim of the present study was to investigate in vitro, whether cytolethality and oxidative damage is enhanced by combination of both mycotoxins as compared to their individual effect. In our paper, we applied a tiered in vitro experimental approach in order to predict the possible health risk effects of two interactive fusarial toxins. Considering the concomitant production of zearalenone (ZEN) and T-2 toxin, it is very likely that humans and animals are always exposed to the mixture rather than to individual compounds.Our results clearly showed that cultured renal cells respond to individual (ZEN) or T-2 toxin exposure by a moderate inhibition of cell proliferation, respectively. However, when combined, they exert a more significant decrease in cell viability. Similar results were found for the investigated oxidative status endpoints. When combined, ZEN and T-2 toxin increased ROS production and heat shock protein (Hsp) 70 expression as compared to the effect of each mycotoxin taken alone.We can conclude that the mixture of ZEN and T-2 toxin increased their toxic effects. The health risk is heightened by the interactions between co-occurring mycotoxins. © 2012 Elsevier GmbH.


Ayed-Boussema I.,Laboratory of Research on Biologically Compatible Compounds | Pascussi J.-M.,French Institute of Health and Medical Research | Maurel P.,French Institute of Health and Medical Research | Bacha H.,Laboratory of Research on Biologically Compatible Compounds | Hassen W.,Laboratory of Research on Biologically Compatible Compounds
International Journal of Toxicology | Year: 2012

Aflatoxin B1 (AFB1), one of the most common mycotoxins found in human foods and animal feed, is principally hepatotoxic and hepatocarcinogenic. The aim of the present study was to explore the effect of AFB1 on messenger RNA (mRNA) expression of pregnane X receptor (PXR), constitutive androstane receptor (CAR), and aryl hydrocarbon receptor (AhR) and some of their target cytochromes using primary cultures of human hepatocytes. Our results showed that AFB1, at noncytotoxic increasing concentrations, caused a significant upregulation of cytochrome P 2B6 (CYP2B6), CYP3A5, and to a lesser extent CYP3A4 and CYP2C9. Pregnane X receptor and CAR mRNA expression increased in the 3 treated livers. Aflatoxin B1 was found also to induce an overexpression of CYP1A1 and CYP1A2 genes accompanied by an increase in AhR mRNA expression. These findings suggest that AFB1 could activate PXR, CAR, and AhR; however, further investigations are needed to confirm nuclear receptor activation by AFB1. © 2011 American College of Toxicology.


El Golli-Bennour E.,Laboratory of Research on Biologically Compatible Compounds
Turkish Journal of Biochemistry | Year: 2011

The decision to attend graduate school to get a PhD is an important one, and not one to be made lightly or without consideration. In Tunisia, typical doctoral programs take four to seven years to complete and in most science fields students then spend two to four years in postdoctoral training before beginning their professional career. Committing to a PhD means sacrifices as doctoral students are generally not well paid and doctoral training is a "job" that requires perseverance and very hard work. Survey on Tunisian doctoral education and career preparation is a national survey of doctoral students intended to provide a snapshot of their experiences and goals. About 200 students completed the 15- questions survey. These students were from 5 selected universities and represented Biomedicine and Health Sciences sectors. In this report, we have tried to explain the reasons making doctoral study as a real abnegation. Hence, there are three major causes (i) doctoral study may increase personal debt: relative to peers, it will forego many years of income (ii) limitation in the opportunity to get international research training courses that approve and finalise dissertations and (iii) Once they obtain their diploma it is too difficult for PhD graduates to get an appropriate work in universities, research or public health establishments. Nevertheless, doctoral work is still usually an exciting and positive experience.


Rjiba-Touati K.,Laboratory of Research on Biologically Compatible Compounds | Ayed-Boussema I.,Laboratory of Research on Biologically Compatible Compounds | Belarbia A.,University of Sousse | Achour A.,University of Sousse | Bacha H.,Laboratory of Research on Biologically Compatible Compounds
Drug and Chemical Toxicology | Year: 2012

Cisplatin (Cisp) is one of the most effective chemotherapeutic agents. However, at higher doses, liver and heart injuries may occur. Recombinant human erythropoietin (rhEPO) has recently been shown to exert an important cytoprotective effect in many tissues. For that reason, we tried to check the protective effect of rhEPO against Cisp-induced genotoxicity and oxidative stress in liver and heart tissues. Our experiments were performed using six groups of adult male Wistar rats. The control group was treated only with saline solution. The rhEPO group was given a single dose of rhEPO. The Cisp group was given a single injection of Cisp. The rhEPO+Cisp groups were given rhEPO simultaneously, 24 hours before, and 5 days after Cisp injection. Our results clearly showed that Cisp induced noticeable DNA damage in the liver and heart, accompanied by a significant increase in protein carbonyl level, reduced glutathione (GSH) depletion, and a decrease in catalase activity. Rats treated with rhEPO, simultaneously, before, or after Cisp injection, remarkably decreased DNA damage. It decreased also the protein carbonyl level, restored GSH depletion, and enhanced catalase activity. Our results highlight an interesting cytoprotective strategy using rhEPO against Cisp-induced liver and heart injuries. © 2012 Informa Healthcare USA, Inc.


Golli-Bennour E.E.,Laboratory of Research on Biologically Compatible Compounds | Bouslimi A.,Laboratory of Research on Biologically Compatible Compounds | Zouaoui O.,Laboratory of Research on Biologically Compatible Compounds | Nouira S.,Laboratory of Research on Biologically Compatible Compounds | And 2 more authors.
Experimental and Toxicologic Pathology | Year: 2012

Amiodarone is a potent anti-arrhythmic drug used for the treatment of cardiac arrhythmias. Although, the effects of amiodarone are well characterized on post-ischemic heart and cardiomyocytes, its toxicity on extra-cardiac tissues is still poorly understood. To this aim, we have monitored the cytotoxicity effects of this drug on three cultured cell lines including hepatocytes (HepG2), epithelial cells (EAhy 926) and renal cells (Vero). We have investigated the effects of amiodarone on (i) cell viabilities, (ii) heat shock protein expressions (Hsp 70) as a parameter of protective and adaptive response and (iii) oxidative damage.Our results clearly showed that amiodarone inhibits cell proliferation, induces an over-expression of Hsp 70 and generates significant amount of reactive oxygen species as measured by lipid peroxidation occurrence. However, toxicity of amiodarone was significantly higher in renal and epithelial cells than in hepatocytes. Vitamin E supplement restores the major part of cell mortalities induced by amiodarone showing that oxidative damage is the predominant toxic effect of the drug.Except its toxicity for the cardiac system, our findings demonstrated that amiodarone can target other tissues. Therefore, kidneys present a high sensibility to this drug which may limit its use with subjects suffering from renal disorders. © 2010 Elsevier GmbH.

Loading Laboratory of Research on Biologically Compatible Compounds collaborators
Loading Laboratory of Research on Biologically Compatible Compounds collaborators