Entity

Time filter

Source Type


Losino N.,Laboratory Of Regulacion Of La Expresion Genica En El Crecimiento Supervivencia Y Diferenciacion Celular | Luzzani C.,Laboratory Of Regulacion Of La Expresion Genica En El Crecimiento Supervivencia Y Diferenciacion Celular | Solari C.,Laboratory Of Regulacion Of La Expresion Genica En El Crecimiento Supervivencia Y Diferenciacion Celular | Boffi J.,Laboratory Of Regulacion Of La Expresion Genica En El Crecimiento Supervivencia Y Diferenciacion Celular | And 8 more authors.
Stem Cells and Development | Year: 2011

Murine embryonic stem cells (mESCs) are pluripotent cells that can be propagated in an undifferentiated state in continuous culture on a feeder layer or without feeders in the presence of leukemia inhibitory factor (LIF). Although there has been a great advance since their establishment, ESC culture is still complex and expensive. Therefore, finding culture conditions that maintain the self-renewal of ESCs, preventing their differentiation and promoting their proliferation, is still an area of great interest. In this work, we studied the effects of the conditioned medium from a bovine granulosa cell line (BGC-CM) on the maintenance of self-renewal and pluripotency of mESCs. We found that this medium is able to maintain mESCs' self-renewal while preserving its critical properties without LIF addition. mESCs cultured in BGC-CM expressed the stem cell markers Oct4, Sox2, Nanog, SSEA-1, Klf4, Rex1, and ECAT1. Moreover, mESCs cultured in BGC-CM gave rise to embryoid bodies and teratomas that differentiated effectively to diverse cell populations from endoderm, mesoderm, and ectoderm. Further, we found that mESCs cultured in BGC-CM have an increased proliferation rate compared with cells grown in the mESC standard culture medium supplemented with LIF. These findings may provide a powerful tool to culture mESCs for long periods of time with high proliferation rate while preserving its basic characteristics, contributing to the application of these cells to assess potential tissue engineering and cellular therapy applications. © Copyright 2011, Mary Ann Liebert, Inc. Source

Discover hidden collaborations