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Zhou Y.,Southwest University | Ming J.,Southwest University | Ming J.,Cornell University | Deng L.,Southwest University | And 2 more authors.
Biological Control | Year: 2014

The separate or combined effects of Pichia membranaefaciens and salicylic acid (SA) on the control of blue and green mold decay in citrus fruits were investigated. Results indicate that combining P. membranaefaciens (1×108CFUml-1) with SA (10μgml-1) either in a point-inoculated or dipped treatment provided a more effective control of blue and green mold than separately applying yeast or SA. SA (10μgml-1) did not significantly affect P. membranaefaciens growth in vitro but slightly increased the yeast population in fruit wounds. P. membranaefaciens plus SA effectively enhanced the phenylalanine ammonia-lyase, peroxidase, polyphenoloxidase, chitinase, and β-1,3-glucanase activities and stimulated the synthesis of phenolic compounds. The combined treatment did not impair quality parameters such as weight loss or titratable acidity, but resulted in low average natural infection incidence and increased total soluble solids and ascorbic acid contents in citrus fruits after 14d at 20°C. © 2014 Elsevier Inc. Source


Du M.,Southwest University | Du M.,Chongqing Special Food Engineering and Technology Research Center | Du M.,Laboratory of Quality and Safety Risk Assessment for Agro products on Storage and Preservation Chongqing | You Y.,Chongqing University of Arts and Sciences | And 4 more authors.
Food Science and Biotechnology | Year: 2015

Variations in amino acid and volatile component contents, and in total flavonoid, polyphenol contents and their vitro antioxidant activities at different aging times of pomelo wine were investigated; scavenging rates against DPPH, ABTS, and ferric reducing antioxidant powers were also explored. Most amino acids reached maximum values in the third aging year; and volatile components of pomelo wine also indicated good wine quality in the third year. Total flavonoid and polyphenol contents in pomelo wine were increased and antioxidant activities enhanced during the first 3 years. Samples aged for 3 years had the highest total flavonoid and total polyphenol contents, and the strongest antioxidant activities (38.23±2.16% for DPPH· scavenging effect, 91.84±4.14% for ABTS· scavenging effect, and 0.90±0.07 mmol/L for FRAP value). These parameters gradually decreased after the 3 year period. A significant (p<0.05) correlation between antioxidant activity, and total flavonoid and polyphenol contents was identified. © 2015, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht. Source


Zhou Y.,Southwest University | Deng L.,Southwest University | Zeng K.,Southwest University | Zeng K.,Laboratory of Quality and Safety Risk Assessment for Agro products on Storage and Preservation Chongqing
Crop Protection | Year: 2014

Effects of hot water treatment (HWT; 53°C for 2min) and Pichia membranaefaciens, either alone or in combination, on controlling Penicillium italicum, Penicillium digitatum, and natural infection in citrus fruit were investigated. Results showed that the combined treatment significantly reduced disease incidence and lesion diameters of blue and green molds in artificially inoculated fruit whether the pathogens were inoculated after or before the treatment compared with the treatment of P. membranaefaciens or HWT alone. The combination of P. membranaefaciens with HWT was as effective as the fungicide treatment in natural infection trials. Application of HWT did not affect the growth of P.membranaefaciens in the wounds of citrus fruit at 20°C or 4°C. P.membranaefaciens combined with HWT effectively enhanced the phenylalanine ammonia-lyase, peroxidase, polyphenoloxidase, chitinase, and β-1,3-glucanase activities and stimulated the synthesis of phenolic compounds. These results suggest that the use of HWT is a useful approach to improve the efficacy of P. membranaefaciens in postharvest diseases of citrus fruit. © 2014 Elsevier Ltd. Source


Zhou Y.,Southwest University | Li S.,Southwest University | Zeng K.,Southwest University | Zeng K.,Laboratory of Quality and Safety Risk Assessment for Agro products on Storage and Preservation Chongqing
Journal of the Science of Food and Agriculture | Year: 2016

BACKGROUND: Nitric oxide (NO) is an important signaling molecule involved in numerous plant responses to biotic and abiotic stresses. To investigate the effects of NO on the control of postharvest anthracnose caused by Colletotrichum gloeosporioides in citrus fruit and its possible mechanisms, citrus fruit were treated with an NO donor. RESULTS: The results showed that exogenous NO released from 50μmol L-1 sodium nitroprusside aqueous solution could effectively reduce the disease incidence and lesion diameter of citrus fruit inoculated with C.gloeosporioides during storage at 20°C. Exogenous NO could regulate hydrogen peroxide levels, stimulate the synthesis of phenolic compounds, and induce phenylalanine ammonia-lyase, peroxidase, polyphenol oxidase, catalase activities, and the ascorbate-glutathione cycle. Furthermore, exogenous NO could inhibit weight loss, improve the ascorbic acid and titratable acidity content, and delay the increase in total soluble solids content in citrus fruit during storage at 20°C. CONCLUSIONS: The results suggest that the use of exogenous NO is a potential method for inducing the disease resistance of fruit to fungal pathogens and for extending the postharvest life of citrus fruit. © 2016 Society of Chemical Industry. Source


Deng L.,Southwest University | Deng L.,Laboratory of Quality and Safety Risk Assessment for Agro products on Storage and Preservation Chongqing | Zhou Y.,Southwest University | Zeng K.,Southwest University | Zeng K.,Laboratory of Quality and Safety Risk Assessment for Agro products on Storage and Preservation Chongqing
Crop Protection | Year: 2015

In this study, the ability of pre-harvest oligochitosan spray to control Colletotrichumgloeosporioides of navel orange during ambient temperature storage was examined. Navel orange trees were sprayed thrice with oligochitosan (15gL-1 water) after physiological fruit drop. Results indicated that disease incidence and lesion diameter were lower in oligochitosan-treated fruit compared with the respective control. The inhibitory effects of volatiles in navel orange rind on the spore germination of C.gloeosporioides were significantly enhanced in the treated fruit compared with the control. Biochemical evaluations revealed that the contents of hydroxyproline-rich glycoprotein were increased. Protopectin degradation was delayed during storage. In addition, the activities of defense-related enzymes, including pectin methylesterase, peroxidase, chitinase, and phenylalanine ammonia-lyase, were increased in oligochitosan-treated navel orange fruit rinds. Our results suggested that pre-harvest oligochitosan spray can be a potential alternative to conventional control methods to prevent post-harvest anthracnose in navel orange. © 2015 Elsevier Ltd. Source

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