Laboratory of Quality Control

Mohammedia, Morocco

Laboratory of Quality Control

Mohammedia, Morocco
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Daouam S.,Research and Development Virology | Daouam S.,Hassan II University | Fakri F.Z.,Research and Development Virology | Ennaji M.M.,Hassan II University | And 4 more authors.
Trials in Vaccinology | Year: 2014

Rift Valley Fever (RVF) is an emerging zoonotic disease present in sub-Saharan Africa and the Arabian Peninsula. Vaccination of cattle against RVF with a RVF virus clone 13 (CL13) strain has proven to be efficacious, and avoids the side effects caused by other available live vaccines. In order to determine the temperature stability of the CL13 vaccine, lyophilized and liquid forms were tested and titrated for the presence of live virus after storage for various time periods at various temperatures. Results showed that the virus could be stored lyophilized at 4 °C for more than 12 months, with no reduction of infectivity. However, the vaccine was shown to be unstable at room temperature and at 37 °C in both lyophilized and liquid forms. This data shows that the CL13 vaccine is highly reliant on a cold chain, emphasizing the need for the vaccine to be made thermostable in order to allow for efficient vaccine storage and delivery in endemic tropical countries. © 2014 Published by Elsevier Ltd.


Fakri F.,Research and Development Virology | Fakri F.,Institute Agronomique et Veterinaire Hassan II | Ghzal F.,Laboratory of Quality Control | Daouam S.,Research and Development Virology | And 5 more authors.
Trials in Vaccinology | Year: 2015

A combined vaccine against Peste des Petits Ruminants (PPR) and Sheep/Goat Pox (SGP) was developed and applied in the field, using a new association of vaccine strains: PPR Nigeria 75 strain with a titre of 104.1 TCID50 and Sheep Pox Romania strain with a titre of 104.0 TCID50. Safety and efficacy were evaluated on goats and sheep in comparison with monovalent PPR and SGP vaccines. Goats were challenged by PPR virulent strain and sheep by SP virulent strain. The result shows that the combined PPR/SGP vaccine confers a good protection against both PPR and SGP infection with no significant difference with monovalent vaccines. The combined vaccine was used in the field on sheep flocks and good sero-conversion was detected for both diseases as soon as 14 days post vaccination. © 2015 Published by Elsevier Ltd.

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