Laboratory of Pharmaco Toxicological Analysis

Castel Guelfo di Bologna, Italy

Laboratory of Pharmaco Toxicological Analysis

Castel Guelfo di Bologna, Italy
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Mandrioli R.,Laboratory of Pharmaco Toxicological Analysis | Mercolini L.,Laboratory of Pharmaco Toxicological Analysis | Raggi M.A.,Laboratory of Pharmaco Toxicological Analysis
Current Drug Metabolism | Year: 2010

A review with 132 references. Several kinds of anxiolytic and hypnotic drugs are currently available on the market. Although BZDs are surely the most frequently prescribed among them, several chemically unrelated compounds have been commercialised, which can provide similar or even higher efficacy and tolerability. These drugs can prove useful for patients who are non-responder or intolerant to benzodiazepine treatment, thus giving broader therapeutic options to the clinician. The most important studies on the metabolic characteristics of several non-benzodiazepine anxiolytics and hypnotics are reported and briefly discussed in this review; moreover, the analytical methods related to these studies are also described and commented upon and their characteristics are highlighted. Finally, an update is included on recent (2007-2010) metabolism and pharmacokinetic studies on benzodiazepines. A monograph is included for each of the following drugs: zolpidem, zaleplon, zopiclone, ramelteon, buspirone and tandospirone; updates are included for the following benzodi-azepines: alprazolam, bromazepam, diazepam, flunitrazepam, lorazepam, midazolam, oxazepam and triazolam. © 2010 Bentham Science Publishers Ltd.


Saracino M.A.,Laboratory of Pharmaco Toxicological Analysis | Tallarico K.,Laboratory of Pharmaco Toxicological Analysis | Raggi M.A.,Laboratory of Pharmaco Toxicological Analysis
Analytica Chimica Acta | Year: 2010

A rapid and reliable analytical method suitable for the simultaneous determination of the antiepileptic drug, oxcarbazepine and its metabolites in human plasma and saliva by means of liquid chromatography with diode array detection (DAD) has been developed. Oxcarbazepine and its metabolites (10,11-dihydro-10-hydroxycarbamazepine, trans-10,11-dihydro-10,11-dihydroxycarbamazepine and 3-hydroxycarbamazepine) were baseline separated within 6.5 min on a reversed-phase C18 column with a phosphate buffer-acetonitrile-triethylamine mixture as the mobile phase. The DAD detector was set at 240 nm. A sample preparation method for biological samples using a microextraction by packed sorbent technique has been implemented, employing a C18 sorbent inserted into a microvolume syringe and using only a small volume (25 μL) of plasma or saliva. The extraction yield values were satisfactory for all analytes (>86.5%) as well as the precision data, which were always in the low percentage of relative standard deviation values (<4.6%). The method was successfully applied to both plasma and saliva samples drawn from psychiatric and neurological patients undergoing treatment with oxcarbazepine (Tolep®) tablets. © 2009 Elsevier B.V. All rights reserved.


Saracino M.A.,University of Bologna | Marcheselli C.,University of Bologna | Somaini L.,Addiction Treatment Center | Pieri M.C.,Addiction Treatment Center | And 3 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

A novel test has been developed for the analysis of methadone in dried blood spot specimens from patients undergoing methadone maintenance treatment. An isocratic reversed-phase high-performance liquid chromatography method with coulometric detection has been optimized for the determination of methadone. The clean-up of dried blood spots was performed by means of an original microextraction by packed sorbent procedure after microwaveassisted extraction of the drug with a suitable solvent. Extraction yields were satisfactory, always being higher than 90.0 %. The calibration curve was linear over the 4- 500 ng mL-1 concentration range. The method had satisfactory sensitivity (limit of quantitation of 4 ng mL-1), precision (relative standard deviation less than 5.8 %), selectivity and accuracy (recovery greater than 87.0 %). It was successfully applied to dried blood spot samples collected from heroinaddicted patients undergoing methadone maintenance therapy at dosages between 40 and 240 mg day-1. The statistical analysis (Bland-Altman plot) showed that the results were in good agreement with those found from the analysis of plasma samples obtained from the same patients. Thus, the method has proved to be suitable for the monitoring of methadone by means of dried blood spots. © Springer-Verlag 2012.

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