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Shawish H.M.A.,University of Palestine | Ghalwa N.A.,Al - Azhar University of Gaza | Al-Dalou A.R.,University of Palestine | Zaggout F.R.,Al - Azhar University of Gaza | And 2 more authors.
Eurasian Journal of Analytical Chemistry | Year: 2011

The detection limit of tramadol hydrochloride was effectively improved by proper selection of a plasticizer and ion exchanger. Two plastic membrane electrodes for the determination of tramadol hydrochloride (TDCl) were fabricated and fully characterized in terms of composition, life span, usable pH range and working concentration range. The membranes of these electrodes consist of an ion-exchanger such as tramadolium-silicotungstate (TD-ST), silicomolybdate (TD-SM), dispersed in PVC matrix with different plasticizers, namely 2-nitrophenyl octyl ether (2-NPOE), dioctyl phthalate (DOP), dibutyl phthalate (DBP), tris(2-ethylhexyl) phosphate (TEPh), dioctyl sebacate (DOS), tributyl phosphate (TBPh) and dibutyl butyl phosphonate (DBBPh). Experiments showed that DBP was the best plasticizer and TD-PT The best ion pair for their combination produced the lowest detection limit. The present electrodes show clear discrimination of tramadol hydrochloride from several inorganic, organic ions, sugars and some common drug excipients. The sensors were applied for determination of tramadol hydrochloride in urine, milk and pharmaceutical preparations using potentiometric determination, standard addition and the calibration curve methods. The results obtained were satisfactory with excellent percentage recovery comparable and sometimes better than those obtained by other routine methods for the assay. © 2011 Moment Publication.


Abu Shawish H.M.,University of Palestine | Saadeh S.M.,Islamic University of Gaza | Al-Dalou A.R.,University of Palestine | Ghalwa N.A.,Al - Azhar University of Gaza | Assi A.A.A.,Laboratory of Pesticide
Materials Science and Engineering C | Year: 2011

Effect of the ISE membrane composition on the characteristics of the tramadol-PVC-electrodes has been investigated. The parameters studied include the effect of the plasticizer and the effect of the ion-pair complex. The plasticizers used were 2-nitrophenyl octyl ether (2-NPOE), dioctyl phthalate (DOP), dibutyl phthalate (DBP), tris(2-ethylhexyl) phosphate (TEPh), dioctyl sebacate (DOS), tributyl phosphate (TBPh) and dibutyl butyl phosphonate (DBBPh) and the ion-pair complexes were tramadolium-silicotungstate (TD-ST), silicomolybdate (TD-SM). These electrodes were fully characterized in terms of composition, life span, usable pH range and working concentration range. The results showed that the best combination was TD-ST as the ion-pair complex and DBP as the plasticizer that produced the electrode with favorable characteristics. Another electrode using TD-SM was tested and produced close results. The present electrodes show clear discrimination of tramadol hydrochloride from several inorganic, organic ions, sugars and some common drug excipients. The sensors were applied for determination of tramadol hydrochloride in urine, milk and pharmaceutical preparations using potentiometric determination, standard addition and the calibration curve methods. The results obtained were satisfactory with excellent percentage recovery comparable and sometimes better than those obtained by other routine methods for the assay. © 2010 Elsevier B.V. All rights reserved.


Abu-Shawish H.M.,University of Palestine | Ghalwa N.A.,Al - Azhar University of Gaza | Zaggout F.R.,Al - Azhar University of Gaza | Saadeh S.M.,Islamic University of Gaza | And 2 more authors.
Biochemical Engineering Journal | Year: 2010

A simple, rapid and sensitive method for the determination of tramadol hydrochloride in urine, milk and pharmaceutical preparations using two modified carbon paste electrodes was developed. One electrode (sensor A) is based on ion-association of tramadol hydrochloride with phosphotungstic acid (TD-PT) and the other (sensor B) with a mixture of phosphotungstic acid (TD-PT) and silicomolybdic acid (TD-SM). Among seven different solvent mediators tested, 2-nitrophenyl octyl ether (NPOE) exhibited a proper behavior including Nernstian slopes of the calibration curve at 57.8 ± 0.4 and 56.5 ± 0.8 mV per decade for sensors A and B. The response times were 8 and 5 s; detection limits 6.2 × 10-6 and 1.8 × 10-6 M; the concentration range 9.2 × 10-6 to 1.0 × 10-1 M and 5.5 × 10-6 to 1.0 × 10-1 M respectively. The present electrodes show good discrimination of tramadol hydrochloride from several inorganic, organic ions, sugars and some common drug excipients. The sensors were applied for the determination of tramadol hydrochloride in urine, milk and pharmaceutical preparations using potentiometric determination, standard addition and the calibration curve methods. The results obtained were satisfactory with excellent percentage recovery comparable and sometimes better than those obtained by other routine methods for the assay. © 2009 Elsevier B.V. All rights reserved.


Duan Y.,Laboratory of Pesticide | Ge C.,Laboratory of Pesticide | Zhang X.,Laboratory of Pesticide | Wang J.,Laboratory of Pesticide | Zhou M.,Laboratory of Pesticide
Australasian Plant Pathology | Year: 2014

The ascomycete Sclerotinia sclerotiorum is a devastating plant pathogen with a very broad host range. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay targeting the Ssos5 sequence for visual detection of S. sclerotiorum. The LAMP reaction was optimal at 63 °C for 45 min. When hydroxynaphthol blue (HNB) was added before amplification, samples with S. sclerotiorum DNA developed a characteristic sky blue colour but those without DNA or with the DNA of nine other plantpathogenic fungi did not. Results obtained with LAMP and HNB were confirmed when LAMP products were subjected to gel electrophoresis. The detection limit of this LAMP assay for S. sclerotiorum was 0.1 fg μL-1 of genomic DNA per reaction, while that of conventional PCR was 100 fg μL-1. Detection results were identical when 13 samples of rapeseed tissue infected with S. sclerotiorum were subjected to LAMP, conventional PCR, and conventional isolation. Because the LAMP assay reported here is simple, rapid, sensitive, and specific, it should be valuable for the detection of S. sclerotiorum in quarantine efforts and field surveys. © Australasian Plant Pathology Society Inc. 2013.

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