Key Laboratory of Pediatrics

Chongqing, China

Key Laboratory of Pediatrics

Chongqing, China
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Hu Y.,Chongqing Medical University | Hu Y.,Key Laboratory of Child Development and Disorders | Hu Y.,Key Laboratory of Pediatrics | Hu Y.,Cooperation Technology | And 8 more authors.
International Journal of Neuroscience | Year: 2012

The objective of this study was to explore the clinical manifestations and electroencephalogram (EEG) features in children with frontal and temporal onset seizures. The method used was video-EEG monitoring that was conducted for 24 h in children with seizure disorders. The results were as follows: There were fewer children with temporal EEG onset seizure (TOS) than with frontal EEG onset seizure (FOS) (p = 0.132). Within the TOS category, PTOS was most frequent, and ATOS was rare (p = 0.001). The mean duration of ATOS was longer than that of TOS and PTOS (p < 0.05). There were no significant differences in seizure frequency and nocturnal attacks between children with TOS and children with FOS. Furthermore, we observed the interictal EEG from three aspects: the background, the location of discharges, and the time of discharges. The frequency of the multi-focal and bilateral discharges of FOS was higher than that of TOS (p < 0.01). The FOS discharged easily and quickly spread to the bilateral hemisphere and formed secondary bilateral synchrony. Focal discharges predominated in TOS, and rarely showed the paroxysm of bilateral synchronous rhythm. Bursts of fast rhythms predominated in the onset of TOS. In contrast, there were a variety of ictal EEG in FOS. Finally, it was concluded that in the group of children studied, the clinical and EEG characteristics of TOS were different from those of FOS. © 2012 Informa Healthcare USA, Inc.

Qin X.,Chongqing Medical University | Qin X.,Ministry of Education Key Laboratory of Child Development and Disorders | Qin X.,Key Laboratory of Pediatrics | Qin X.,Cooperation Technology | And 12 more authors.
Archives of Virology | Year: 2013

Human respiratory syncytial virus (RSV) is recognized as a leading pathogen responsible for severe respiratory infections in the pediatric population, particularly in infants and young children. A previous study by the same study group revealed that the RSV BA strain was prevalent in southwestern China in epidemic seasons from 2008 to 2009. The aim of this study was to investigate the epidemiology of RSV in the following two years, the genetic variability of the G gene, and mutations at the 276th amino acid in the fusion (F) protein of RSV strains. Nine BA substrains were found in 16 subgroup B viruses by phylogenetic analysis. The G gene of genotype BA was predicted to encode proteins with five different lengths. The findings indicate that subgroup A and B RSVs alternately circulate in southwestern China and that genotype BA strains appear to be the long-term circulating ones. These epidemiological data may help in future vaccine design and further investigation of G protein function. © 2013 Springer-Verlag Wien.

Lai F.-F.,Chongqing Medical University | Lai F.-F.,Key Laboratory of Pediatrics | Yan Q.,Xiamen University | Ge S.-X.,Xiamen University | And 6 more authors.
Journal of Medical Virology | Year: 2016

Hand, foot, and mouth disease (HFMD) has become very common in children, with widespread occurrence across China. The aim of this study was to investigate the epidemiologic and etiologic characteristics of HFMD, including etiologic variations in Chongqing, China. An epidemiologic investigation was based on 3,472 patients who presented with HFMD manifestations and were admitted at the Children's Hospital of Chongqing Medical University between 2010 and 2013. Fecal specimens from 830 patients were analyzed by nested RT-PCR to identify the enterovirus pathogens, and the molecular characterization of HFMD was illustrated by phylogenetic tree analysis. The results of this study indicate that the peak of the HFMD epidemic in Chongqing between 2010 and 2013 occurred between April and July each year. The median age of onset was 2.24 years old, and children under the age of five accounted for 96.4% of all the HFMD cases; the male-to-female ratio was 1.89:1. Enterovirus 71 accounted for a major proportion of the isolated strains every year, including the majority (74%) of severe cases. However, the proportion of Coxsackie A (CV-A) 6 infections increased from 2.11% in 2010 to 16.36% in 2013, while the proportion of CV-A16 infections decreased from 31.23% in 2010 to 4.67% in 2013. Molecular epidemiologic study showed that all enterovirus 71 strains belonged to subgenotype C4a, whereas all CV-A16 strains belonged to genotype B1, including subgenotype B1a and subgenotype B1b. © 2016 Wiley Periodicals, Inc.

Qin X.,Chongqing Medical University | Qin X.,Ministry of Education Key Laboratory of Child Development and Disorders | Qin X.,Key Laboratory of Pediatrics | Qin X.,Cooperation Technology | And 20 more authors.
World Journal of Pediatrics | Year: 2013

Background: X-linked agammagobulinemia (XLA) is a primary immunodeficiency caused by Bruton's tyrosine kinase (BTK) gene mutation. XLA patients have an extremely small amount of peripheral B cells and profound deficiency in all immunoglobulin isotypes. We analyzed the clinical, immunologic, and molecular characteristics of children with XLA in an attempt to improve the diagnosis and treatment of XLA in China. Methods: Twenty children with XLA-compatible phenotypes from 18 unrelated families were enrolled in this study. The BTK gene was amplified and sequenced, followed by mutation analysis in these children and their female relatives. Results: Eighteen different mutations of the BTK gene were identified in the 20 patients. Eleven mutations had been reported previously including eight missense mutations (c.994C>T, c.1987C>A, c.1885G>T, c.502T>C, c.1085C>T, c.1816C>T, c.214C>T, c.1912G>A) and three nonsense mutations (c.1267T>A, c.1793C>G, c.1618C>T). Seven novel mutations of the BTK gene were also presented and included five missense mutations (c.134T>A, c.1646T>A, c.1829C>G, c.711G>T, c.1235G>A), one splice-site mutation (c.523+1G>A) and one insertion mutation (c.1024-1025in sTTGCTAAAGCAACTGCTAAAGCAAG). Eight out of 18 mutations of the BTK gene were located in the TK domain, 4 in the PH domain, 4 in the SH2 domain and 2 in the TH domain. Genetic study for carrier status was carried out in 18 families with definite BTK gene mutations. Nine carriers with BTK gene mutations were identified. Six families without carriers were detected, and 3 patients were not tested in this study. Conclusion: Our results support that molecular genetic testing represents an important tool for early confirmed diagnosis of congenital agammaglobulinemia and may allow accurate carrier detection and prenatal diagnosis. © 2013 Children's Hospital, Zhejiang University School of Medicine and Springer-Verlag Berlin Heidelberg.

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