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Andritoiu C.V.,Vasile Goldis Western University of Arad | Andritoiu C.V.,Technical University Gheorghe Asachi | Andritoiu C.V.,Apitherapy Medical Center | Bartic M.,Apitherapy Medical Center | And 2 more authors.
Annals of the Romanian Society for Cell Biology | Year: 2015

This experiment studies the therapeutic effect of a new ointment based on lyophilized albumin extracted from egg on cutaneous excision lesion. The therapeutical assessments of the proposed ointment were done in vivo on the excisions excided on Wistar rat. The wounds were treated topically once a day, along 21 days. Clinical and macroscopic tests, tissue epitalization time wound contraction rate and histopathological examination were evaluated. The analyzes results revealed a significant healing effect of the new lyophized-albumin on the skin excision lesions by stimulation of the regeneration and the maturation of granulation tissue, antiinflammatory effect, collagenation effect at the lesion site resulting an incision-like mark hard to be seen without vicious healing in terms of both the clinic, macroscopic and histological point of view. © The Romanian Society for Cell Biology. Source

Sava I.,Grigore T. Popa University of Medicine and Pharmacy | Sava A.,Grigore T. Popa University of Medicine and Pharmacy | Sapte E.,Ovidius University | Mihailov C.,Ovidius University | And 4 more authors.
Romanian Journal of Morphology and Embryology | Year: 2013

Background: Intraventricular tumors represent a diagnostic problem, due to a wide range of differential diagnosis, with an important variability of tumoral histological types in adult and pediatric population. Patient, Methods and Results: Our case is represented by a patient, aged 48 years, without any history of significant personal pathology, accusing nausea, vomiting, and intensive headache. In the morning, he became confused, having hallucinations for a short period of time, and has accused drowsiness for several weeks. Imaging (CT and MRI) shows a neoformation in the third ventricle, accompanied by bilateral lateral ventricles dilatation, with predominantly annular enhancement. During surgery, through the middle third transcallosal interhemispheric approach, it was revealed a reddish, well-demarcated intraventricular mass, well vascularized and with a firm consistency. Final pathologic diagnosis was metastatic clear cell renal carcinoma. Initial postoperative evolution was good, and then neurological and respiratory condition worsened as a bronchopneumonia lead to patient's death in 12 days after surgery. Conclusions: Clear cell carcinoma metastasis located in the third ventricle should be taken into consideration for patients presenting a single intraventricular lesion even they have no documented primary malignancy. Source

Sava A.,Grigore T. Popa University of Medicine and Pharmacy | Dumitrescu G.,Laboratory of Pathological Anatomy | Haba D.,Grigore T. Popa University of Medicine and Pharmacy | Hodorog D.,Grigore T. Popa University of Medicine and Pharmacy | And 2 more authors.
Romanian Journal of Morphology and Embryology | Year: 2013

Fahr syndrome (FS) refers to basal ganglia calcification that is associated with many neurological and psychiatric abnormalities and appears as secondary to other diseases. We described a case of FS patient who was admitted in the Department of Neurology of "Prof. Dr. Nicolae Oblu" Clinical Emergency Hospital, Iassy, Romania, with seizure and mood disorders. On CT, the cause of seizure was found to be the bilateral calcifications of cerebellum, basal ganglia, thalamus and internal capsule. As the patient died after 15 days of hospitalization due to new seizures and gastrointestinal infection, an autopsy was made. Grossly, there were bilateral symmetrically gritty yellow areas in basal ganglia, thalami, internal capsule, cerebral cortex, cerebellar folia, dentate nucleus, and brain stem. A detailed histopathological examination revealed five types of calcium deposits within the walls of capillaries, small and medium-sized arteries from the intracerebral affected areas, chronic lymphocytic thyroiditis and fibro-adipose tissue instead of parathyroids. We consider that intracerebral symmetrical calcifications were the results of the hypoparathyroidism determined by an ancient autoimmune parathyroiditis that evolved to fibrosis as at microscopy we found an autoimmune thyroiditis. Source

Adamkov M.,Comenius University | Halasova E.,Comenius University | Kajo K.,Biocyt Diagnostic Center | Machalekova K.,Biocyt Diagnostic Center | And 3 more authors.
Neoplasma | Year: 2010

The antiapoptotic protein survivin can be detected in most types of malignant tumors, but it is rarely expressed in corresponding normal adult tissues. Therefore, survivin appears to represent a promising diagnostic biomarker. We examined survivin expression in 13 cases of normal breast tissue, 38 cases of fibroadenomas and 80 cases of breast carcinomas by immunohistochemical staining using anti-survivin antibody (DAKO, Clone 12C4). In each section, the intensity of staining, percentage of labeled cells, and the subcellular location of survivin antigen were assessed. Survivin was detected in 4/13 cases of normal breast tissue (30.7%), in 28/38 cases of fibroadenomas (73.7%), and in 67/80 cases of carcinomas (83.8%). Normal breast tissue showed cytoplasmic positivity only. In fibroadenomas, 19 cases (50.0%) revealed cytoplasmic reaction, and in 9 cases (23.7%), small foci of cells with combined nuclear and cytoplasmic location were identified. In carcinomas, cytoplasmic staining was found in 12/80 cases (15.0%), nuclear staining in 10/80 cases (12.5%), and combined cytoplasmic and nuclear staining in 45/80 cases (56.3%). Subcellular location of survivin between benign and malignant lesions revealed significant differences (p<0,001). Our findings point at practical use of survivin detection. We confirm the importance of nuclear staining of survivin antigen in breast carcinoma, which seems to be a notable diagnostic marker for estimation of the degree of neoplasia. Source

Adamkov M.,Comenius University | Kajo K.,Biocyt Diagnostic Center | Vybohova D.,Comenius University | Krajcovic J.,Comenius University | And 2 more authors.
Neoplasma | Year: 2012

The antiapoptotic protein survivin is widely expressed in most human cancers, including carcinomas of the breast. It is rarely detected in corresponding normal adult tissues. Therefore, survivin comes into the limelight as a promising diagnostic biomarker and prognostic parameter. Immunohistochemically, we examined the expression of this protein in 126 cases of ductal breast carcinoma to determine the association with clinicomorphological parameters such as age of patients, grade, stage and size of the primary tumor, lymph node metastasis, vascular invasion as well as estrogen and progesterone status. In each section, the subcellular location of survivin antigen, the intensity of staining and the percentage of labeled cells were assessed. Overall, survivin was expressed in 111 cases (88.1%). The statistical analysis revealed a significant correlation between the nuclear location of survivin and tumor grade 3. Furthermore, a significant relation was also found between vascular invasion and nuclear and combined nuclear and cytoplasmic survivin expression, together with a higher intensity of immunoreaction. However, no significant correlations were shown with other clinicomorphological parameters, such as stage and size of the tumor, lymph node metastasis, estrogen and progesterone receptors and age. Our findings revealed that survivin was frequently overexpressed in carcinoma cells, where it was present in different subcellular compartments. The nuclear positivity of survivin or combined nuclear and cytoplasmic expression was shown to be a poor prognostic parameter in ductal breast carcinoma. Source

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