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Yamamoto T.,Laboratory of Molecular Functions of Food | Takahashi H.,Laboratory of Molecular Functions of Food | Goto T.,Laboratory of Molecular Functions of Food | Takahashi N.,Laboratory of Molecular Functions of Food | Kawada T.,Laboratory of Molecular Functions of Food
Bioscience, Biotechnology and Biochemistry | Year: 2014

Several concentrations of theobromine (TB) and (-)-epicatechin (EC) were coadministered to rats, and plasma EC and its metabolites were determined using ultra-high-performance liquid chromatography-tandem mass spectrometry. It has been demonstrated that TB increases the absorption of EC in a dose-dependent manner. Cocoa powder had a similar effect, and the mechanism involved is not thought to depend on tight junctions. © 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.


Takahashi H.,Laboratory of Molecular Functions of Food | Suzuki H.,Kazusa DNA Research Institute | Suda K.,Kazusa DNA Research Institute | Yamazaki Y.,Laboratory of Molecular Functions of Food | And 11 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2013

A change in the free fatty acid (FFA) profile reflects an alteration in the lipid metabolism of peripheral tissue. A high-throughput quantitative analysis method for individual FFAs therefore needs to be established. We report here an optimized LC-MS assay for a highthroughput and high-sensitivity analysis of the 10 major long-chain FFAs in mouse plasma and liver. This assay enables quantification of individual FFAs by using trace amounts of samples (2μL of plasma and 10 mg of liver tissue). We apply this method to analyze the FFA profile of plasma and liver samples from an obese mouse model treated with bezafibrate, the peroxisome proliferatoractivated receptor α (PPARα) agonist, and show a change in the FFA profile, particularly in the palmitoleic and oleic acid contents. This assay is useful for quantifying individual FFAs and helpful for monitoring the condition of lipid metabolism.

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