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Tagliamonte M.,Laboratory of Molecular Biology and Viral Oncogenesis | Visciano M.L.,Laboratory of Molecular Biology and Viral Oncogenesis | Tornesello M.L.,Laboratory of Molecular Biology and Viral Oncogenesis | De Stradis A.,National Res Council Institute Plant Virology | And 3 more authors.
Vaccine | Year: 2011

We have previously described the establishment and characterization of a stably transfected insect cell line for the constitutive and efficient expression of Pr55 HIV Gag proteins, which auto-assemble into enveloped Virus-Like Particles (VLPs) released into the cell culture supernatant. Such HIV-Gag VLPs have been shown to elicit a specific systemic humoral response in vivo, proving the appropriate antigenic presentation of the HIV Gag protein to the immune system. Here we describe the establishment of a stable double transfected insect cell line for the constitutive and reproducible production of Pr55Gag-VLPs expressing on their surface trimeric forms of HIV-1 envelope glycoproteins. The persistence of HIV coding genes has been verified in clonal resistant insect cells, the protein expression and conformation has been verified by Western blot analysis. The resulting HIV-VLPs have been visualized by standard transmission electron microscopy and their immunogenicity has been evaluated in vivo. This represents, to our knowledge, the first example of stable double transfected insect cell line for the constitutive production of enveloped HIV-Gag VLPs presenting trimeric HIV-gp140 on their surface. © 2011 Elsevier Ltd. Source


Shoja Z.,Tehran University of Medical Sciences | Tagliamonte M.,Laboratory of Molecular Biology and Viral Oncogenesis | Jalilvand S.,Tehran University of Medical Sciences | Ziaee A.-A.,University of Tehran | And 8 more authors.
New Microbiologica | Year: 2012

The full open reading frame of the outer protein layer VP7 from an isolate of human rotavirus identified in 2010 in an Iranian child admitted to hospital with gastroenteritis was amplified from a clinical stool specimen and subjected to molecular characterization. Genetic and phylogenetic analyses indicated that the analyzed gene falls into the G1 genotype forming a sub-cluster with sequences recently identified in Iran and geographically distant countries. Such results were confirmed by protein sequence alignment, showing a highly conserved "G1-like" amino acid sequence pattern within the known three main immunodominant regions. These results are extremely relevant in a perspective of vaccine development. Indeed, the present study confirms that the A group G1 genotype is the most prevalent Rotavirus circulating in Iran and supports the development of G1 genotype-based rotavirus vaccine for this country. Source


Tuccillo F.M.,Laboratory of Molecular Biology and Viral Oncogenesis | Palmieri C.,University of Catanzaro | Fiume G.,University of Catanzaro | De Laurentiis A.,University of Catanzaro | And 17 more authors.
Molecular Cancer Therapeutics | Year: 2014

CD43 is a sialoglycosylated membrane protein that is involved in cell proliferation and differentiation. CD43 glycoforms that are recognized by the UN1 monoclonal antibody (mAb) were expressed in lymphoblastoid T-cell lines and solid tumors, such as breast, colon, gastric, and squamous cell lung carcinomas, while unexpressed in the normal counterparts. The cancer association of UN1/CD43 epitope suggested the possibility to use the UN1 mAb for tumor diagnosis and therapy. In this study, we show that the UN1 mAb was endowed with antitumor activity in vivo because its passive transfer inhibited the growth of UN1-positive HPB-ALL lymphoblastoid T cells in mice. Furthermore, we demonstrate that tumor inhibition was due to UN1 mAb-dependent natural killer-mediated cytotoxicity. By screening a phage-displayed random peptide library, we identified the phagotope 2/165 as a mimotope of the UN1 antigen, as it harbored a peptide sequence that was specifically recognized by the UN1 mAb and inhibited the binding of the UN1 mAbto UN1-positive tumor cells. On the basis of sequence homology with the extracellular region of CD43 (amino acids 64 to 83), the 2/165 peptide sequence was likely mimicking the protein core of the UN1/CD43 epitope. When used as vaccine in mice, the 2/165 phagotope raised antibodies against the UN1/CD43 antigen, indicating that the 2/165 phagotope mimicked the UN1 antigen structure, and could represent a novel immunogen for cancer immunotherapy. These findings support the feasibility of using monoclonal antibodies to identify cancer-associated mimotopes for immunotherapy. © 2013 American Association for Cancer Research. Source


Buonaguro L.,Laboratory of Molecular Biology and Viral Oncogenesis | Tagliamonte M.,Laboratory of Molecular Biology and Viral Oncogenesis | Visciano M.L.,Laboratory of Molecular Biology and Viral Oncogenesis | Andersen H.,BIOQUAL Inc. | And 7 more authors.
Clinical and Vaccine Immunology | Year: 2012

Female rhesus macaques were immunized with HIV virus-like particles (HIV-VLPs) or HIV DNA administered as sequential combinations of mucosal (intranasal) and systemic (intramuscular) routes, according to homologous or heterologous primeboost schedules. The results show that in rhesus macaques only the sequential intranasal and intramuscular administration of HIV-VLPs, and not the intranasal alone, is able to elicit humoral immune response at the systemic as well as the vaginal level. Copyright © 2012, American Society for Microbiology. All Rights Reserved. Source


Visciano M.L.,Laboratory of Molecular Biology and Viral Oncogenesis | Tagliamonte M.,Laboratory of Molecular Biology and Viral Oncogenesis | Tornesello M.L.,Laboratory of Molecular Biology and Viral Oncogenesis | Buonaguro F.M.,Laboratory of Molecular Biology and Viral Oncogenesis | Buonaguro L.,Laboratory of Molecular Biology and Viral Oncogenesis
Journal of Translational Medicine | Year: 2012

Background: Virus-Like Particles (VLPs) represent an efficient strategy to present and deliver conformational antigens to the immune system, inducing both arms of the adaptive immune response. Moreover, their particulate structure surrounded by cell membrane provides an adjuvanted effect to VLP-based immunizations. In the present study, the elicitation of different patterns of IgG subclasses by VLPs, administered in CpG ODN1826 or poly(I:C) adjuvants, has been evaluated in an animal model.Results: Adjuvanted VLPs elicited a higher titer of total specific IgG compared to VLPs alone. Furthermore, while VLPs alone induced a balanced T H2 pattern, VLPs formulated with either adjuvant elicited a T H1-biased IgG subclasses (IgG2a and IgG3), with poly(I:C) more potent than CpG ODN1826.Conclusions: The results confirmed that adjuvants efficiently improve antigen immunogenicity and represent a suitable strategy to skew the adaptive immune response toward the differentiation of the desired T helper subset, also using VLPs as antigen. © 2012 Visciano et al; licensee BioMed Central Ltd. Source

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