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Messora M.R.,University of Sao Paulo | Pereira L.J.,Federal University of Lavras | Foureaux R.,Federal University of Lavras | Oliveira L.F.F.,University of Sao Paulo | And 6 more authors.
Archives of Oral Biology | Year: 2016

Objectives The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1 - E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2 - E2). Methods In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2. Results In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P < 0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P < 0.05). Conclusions Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats. © 2016 Elsevier Ltd. All rights reserved.

De C. Foureaux R.,Federal University of Lavras | Messora M.R.,University of Sao Paulo | De Oliveira L.F.F.,University of Sao Paulo | Napimoga M.H.,Laboratory of Immunology and Molecular Biology | And 3 more authors.
Journal of Periodontology | Year: 2014

Background: This study evaluates the effects of probiotic therapy (PT) in rats with ligature-induced periodontitis associated with restraint stress. Methods: Sixty-four rats were divided into control, stress (STR), probiotic (PROB), periodontal disease (PD), STRPROB, STR-PD, STR-PROB-PD, and PROB-PD groups. The probiotic was added to the drinking water for 44 days. PD was induced by a ligature. In STR groups, the animals were subjected to restraint stress for 2.5 hours per day for 30 days. Results: Rats with PD exhibited increased alveolar bone loss (P <0.05), as well as increased levels of cyclooxygenase-2, serum C-terminal telopeptide (CTX), p38 mitogen-activated protein kinase (p38), and receptor activator of nuclear factor-kB ligand and decreased levels of osteoprotegerin (OPG). Stressed rats presented high levels of C-peptide, corticosterone, and glucose (P <0.05). In general, the presence of stress reduced the expression of CTX and p38 (P <0.05). PT reduced alveolar bone loss in unstressed animals. It also decreased expression of CTX and induced increased expression of OPG in unstressed animals with PD. However, PT was not effective in preventing bone loss or altering the expression of inflammatory markers in stressed animals. PT decreased the number of inflammatory cells in the periodontal tissue (P <0.05). Groups with stress and PD showed decreased villous height and crypt depth. Stress seemed to prevent part of the probiotic beneficial effects on the small intestine. Conclusions: Based on the methodology used, PT may reduce tissue breakdown resulting from PD in unstressed rats. The protocol used for restraint stress influenced the immunomodulatory effects of PT in intestinal and periodontal tissues.

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