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Mahjoub M.A.,University of Monastir | Mighri Z.,University of Monastir | Chriaa J.,Laboratory of Environment Microbiology | Daami M.,Regional Pole of Agricultural Research 6 Development | And 2 more authors.
Journal of Essential Oil Research | Year: 2010

Essential oils from the aerial parts of Frankenia laevis were obtained by steam distillation and were analyzed by GC-FID and GC/MS. Forty-four compounds were identified, representing 91.5% of the oil constituents. Hexadecanoic acid was the major compound (19.8%), followed by methyl linoleate (16%), (E, E)-farnesyl acetate (9.5%), (E)- nerolidol (8.9%) and benzyl benzoate (6.8%). Minimum inhibitory concentrations, minimum bactericidal concentrations and inhibition zone diameters against grain-positive bacteria, gram-negative bacteria and phytopathogenic fungi were determined. Essential oil was particularly effective against Staphylococcus aureus, Micrococcus luteus and Salmonella typhimurium, nevertheless no antifungal activity was detected at the tested concentrations. © 2010 Allured Business Media.

Bahri-Sahloul R.,Tunis el Manar University | Ben Fredj R.,University of Sousse | Boughalleb N.,Higher Institute of Agronomy of Chott Mariem | Shriaa J.,Laboratory of Environment Microbiology | And 6 more authors.
Journal of Botany | Year: 2014

Objective. Plant cell culture is an innovative technology to produce a variety of substances. Numerous plants synthesize among their secondary metabolites phenolic compounds which possess antioxidant and antimicrobial effects. Hawthorn (Crataegus) is one of these plants which has long been used in folk medicine and is widely utilized in pharmaceutical preparations mainly in neuro- and cardiosedative actions. Methods and Results. The production of polyphenol by fifty-two-week-old Crataegus azarolus var. aronia calli was studied in relation to growth variation and antioxidant and antimicrobial capacity within a subcultured period. The DPPH and ABTS+ assays were used to characterize the antioxidant actions of the callus cultures. Antimicrobial activity was tested by using disc diffusion and dilution assays for the determination of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values of each active extract. High TEAC TEAC and antimicrobial activity was observed when maximal growth was reached. An optimum of total phenol, proanthocyanidins, flavonoid, (-)-epicatechin, procyanidin B2, chlorogenic acid, and hyperoside was produced during this period. Conclusion. Antioxidant and antimicrobial activities were strongly correlated with total phenols and total flavonoids. Crataegus azarolus var. aronia cells culture represents an important alternative source of natural antioxidants and antimicrobials. © 2014 Radhia Bahri-Sahloul et al.

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