Laboratory of Microbial Biotechnology

Fès, Morocco

Laboratory of Microbial Biotechnology

Fès, Morocco
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Sadiki M.,Laboratory of Microbial Biotechnology | Balouiri M.,Laboratory of Microbial Biotechnology | Barkai H.,Laboratory of Microbial Biotechnology | Maataoui H.,Laboratory of Microbial Biotechnology | And 4 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: The present study attempts to investigate in vitro antibacterial activity of M. communis and T. vulgaris essential oils alone and in combination against two bacterial strains Methods: The antibacterial activity of M. communis and T. vulgaris essential oils was assessed against Staphylococcus aureus and Escherichia coli by using the broth microdilution method then the fractional inhibitory concentration (FIC) index was used to define the interactions between both essential oils. Results: Our results show that T. vulgaris was more effective compared to M. communis when both essential oils are tested alone against bacterial strains studied. Moreover, all applications of combination between M. communis and T. vulgaris essential oils displayed a partial synergistic antibacterial effect. Only, one combination against S. aureus and two combinations against E. coli were proved highly synergistic effect, increasing the antibacterial activity. Conclusion: The antibacterial activity of both essential oils was enhanced by the combination (1/8 MIC Myrtle +1/4 MIC thyme) and two combinations (1/4 MIC Myrtle + 1/8 MIC thyme and 1/8 MIC myrtle + 1/4 MIC thyme) which proved a highly synergistic effect against S. aureus ATCC 25922 and E. coli O128B12 respectively. These findings suggest that the mixture of these essential oils at suitably low concentrations could be a promising alternative to replace synthetic antimicrobial agents and lead to new research about natural products to enhancing its antibacterial properties.


Nakkabi A.,Moulay Ismaï University | Sadiki M.,Laboratory of Microbial Biotechnology | Fahim M.,Moulay Ismaï University | Ittobane N.,Moulay Ismaï University | And 5 more authors.
International Journal of Environmental Research | Year: 2015

Polyurethanes (PURs) are polymers that can be generated by a step-growth polyaddition reaction of diisocyanates with polyols. The number of well-developed chemical procedures applicable to their manufacture, and the diversity of chemical structures in which polyurethanes can be built, account for the widespread use of these materials in the last few decades for both domestic and technical applications due to its excellent mechanical and thermal properties and their high resistance to the climatic changes. Nevertheless, its high resistance to degradation in aqueous media or by microorganism constitutes not only a great disadvantage for its use as material in medical applications (vascular grafts, artificial heart diaphragms, valves, catheters…) but also as one of the sources of the pollution of the environment. In this work, we report the degradation of a commercial poly (ester-urethane) by microorganisms isolated from cedar wood. This is the first study that demonstrates the degradation of polyurethane by isolated microorganisms from wood. Analyses were carried out by Infrared spectroscopy. © 2015, University of Tehran. All rights reserved.

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