Laboratory of Cell Metabolism

Leuven, Belgium

Laboratory of Cell Metabolism

Leuven, Belgium

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Crick P.J.,University of Swansea | Crick P.J.,Swiss Tropical and Public Health Institute | Beckers L.,Laboratory of Cell Metabolism | Baes M.,Laboratory of Cell Metabolism | And 3 more authors.
Steroids | Year: 2015

Abstract Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5 pg/mL-2.6 ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma. © 2015 The Authors.


Peeters A.,Laboratory of Cell Metabolism | Swinnen J.V.,Catholic University of Leuven | Van Veldhoven P.P.,University of Sfax | Baes M.,Laboratory of Cell Metabolism
Biochimie | Year: 2011

Peroxisome deficiency in liver causes hepatosteatosis both in patients and in mice. Here, we studied the mechanisms that contribute to this lipid accumulation and to activation of peroxisome proliferator activated receptor α (PPARα) by using liver-specific Pex5 -/- mice (L-Pex5 -/- mice). Surprisingly, steatosis was accompanied both by increased mitochondrial β-oxidation capacity, confirming previous observations, and by impaired de novo lipid synthesis mediated by reduced expression of sterol regulatory element binding protein 1c and its targets. As a consequence, when challenged with a high fat diet, L-Pex5 -/- mice were protected from adiposity. Hepatic fatty acid uptake was strongly increased whereas the expression of apolipoproteins and the lipoprotein assembly factor microsomal triglyceride transfer protein were markedly reduced resulting in reduced secretion of very low density lipoproteins. Most of these changes seemed to be orchestrated by the endogenous activation of PPARα, challenging the assumption that PPARα activation in hepatocytes requires fatty acid synthase dependent de novo fatty acid synthesis. Expression of cholesterol synthesizing enzymes and cholesterol levels were not affected in peroxisome deficient liver. In conclusion, increased fatty acid uptake driven by endogenous PPARα activation and reduced fatty acid secretion cause hepatosteatosis in peroxisome deficient livers. © 2011 Elsevier Masson SAS. All rights reserved.


Crick P.J.,Swiss Tropical and Public Health Institute | Beckers L.,Laboratory of Cell Metabolism | Baes M.,Laboratory of Cell Metabolism | Van Veldhoven P.P.,LIPIT | And 2 more authors.
Steroids | Year: 2015

Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5. pg/mL-2.6. ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma. © 2015 The Authors.


PubMed | Laboratory of Cell Metabolism, University of Swansea and LIPIT
Type: Journal Article | Journal: Steroids | Year: 2015

Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5pg/mL-2.6ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma.

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