Laboratory of Medical Biology

Saint-Mandé, France

Laboratory of Medical Biology

Saint-Mandé, France
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Szulc P.,University of Lyon | Amri E.Z.,University of Nice Sophia Antipolis | Amri E.Z.,French Institute of Health and Medical Research | Varennes A.,Laboratory of Medical Biology | And 6 more authors.
Diabetes Research and Clinical Practice | Year: 2016

Aim Oxytocin regulates food intake, carbohydrate and lipid metabolism, and urinary sodium excretion. We assessed the association between serum oxytocin levels and presence of metabolic syndrome (MetS) in older men. Methods Cross-sectional study was performed in 540 volunteer men aged 50–85 yrs from the MINOS cohort. Oxytocin was measured in fasting serum by radioimmunoassay (Oxytocin RIA, Phoenix Pharmaceuticals). MetS was diagnosed using the harmonized definition. Results Serum oxytocin was higher in 166 men with MetS vs. controls (p < 0.005). After adjustment for confounders including leptin, higher oxytocin was associated with higher odds of MetS (OR = 1.38 per SD, 95%CI: 1.10–1.71, p < 0.005). Men with serum oxytocin >0.74 pg/mL (median) had higher odds of MetS vs. men with oxytocin ⩽0.74 pg/mL (OR = 2.06, 95%CI: 1.33–3.18, p < 0.005). Higher oxytocin levels and low testosterone levels (total or free) were significantly associated with higher odds of MetS jointly and independently of each other. Men having oxytocin >0.74 pg/mL and total testosterone <300 ng/dL (<10.4 nmol/L) had higher odds of MetS vs. men without these characteristics (OR = 3.95, 95%CI: 1.65–9.46, p < 0.005). Men having 25-hydroxycholecalciferol levels <30 ng/mL and oxytocin >0.74 pg/mL had higher odds of MetS vs. men without these characteristics (OR = 2.86, 95%CI: 1.47–5.58, p < 0.01). Men having oxytocin >0.74 pg/mL and osteocalcin levels <14.6 ng/mL (lowest quartile) had higher odds of MetS vs. men without these characteristics (OR = 4.12, 95%CI: 2.07–8.20, p < 0.001). Conclusion In older men, higher serum oxytocin levels are associated with higher odds of MetS regardless of potential confounders. © 2016 Elsevier Ireland Ltd


PubMed | University of Nice Sophia Antipolis, Nice University Hospital Center, University of Lyon and Laboratory of Medical Biology
Type: | Journal: Diabetes research and clinical practice | Year: 2016

Oxytocin regulates food intake, carbohydrate and lipid metabolism, and urinary sodium excretion. We assessed the association between serum oxytocin levels and presence of metabolic syndrome (MetS) in older men.Cross-sectional study was performed in 540 volunteer men aged 50-85yrs from the MINOS cohort. Oxytocin was measured in fasting serum by radioimmunoassay (Oxytocin RIA, Phoenix Pharmaceuticals). MetS was diagnosed using the harmonized definition.Serum oxytocin was higher in 166 men with MetS vs. controls (p<0.005). After adjustment for confounders including leptin, higher oxytocin was associated with higher odds of MetS (OR=1.38 per SD, 95%CI: 1.10-1.71, p<0.005). Men with serum oxytocin >0.74pg/mL (median) had higher odds of MetS vs. men with oxytocin 0.74pg/mL (OR=2.06, 95%CI: 1.33-3.18, p<0.005). Higher oxytocin levels and low testosterone levels (total or free) were significantly associated with higher odds of MetS jointly and independently of each other. Men having oxytocin >0.74pg/mL and total testosterone <300ng/dL (<10.4nmol/L) had higher odds of MetS vs. men without these characteristics (OR=3.95, 95%CI: 1.65-9.46, p<0.005). Men having 25-hydroxycholecalciferol levels <30ng/mL and oxytocin >0.74pg/mL had higher odds of MetS vs. men without these characteristics (OR=2.86, 95%CI: 1.47-5.58, p<0.01). Men having oxytocin >0.74pg/mL and osteocalcin levels <14.6ng/mL (lowest quartile) had higher odds of MetS vs. men without these characteristics (OR=4.12, 95%CI: 2.07-8.20, p<0.001).In older men, higher serum oxytocin levels are associated with higher odds of MetS regardless of potential confounders.


Fadhlaoui-Zid K.,Tunis el Manar University | Rodriguez-Botigue L.,University Pompeu Fabra | Naoui N.,Laboratory of Medical Biology | Benammar-Elgaaied A.,Tunis el Manar University | And 4 more authors.
American Journal of Physical Anthropology | Year: 2011

Human population movements in North Africa have been mostly restricted to an east-west direction due to the geographical barriers imposed by the Sahara Desert and the Mediterranean Sea. Although these barriers have not completely impeded human migrations, genetic studies have shown that an east-west genetic gradient exists. However, the lack of genetic information of certain geographical areas and the focus of some studies in parts of the North African landscape have limited the global view of the genetic pool of North African populations. To provide a global view of the North African genetic landscape and population structure, we have analyzed ∼2,300 North African mitochondrial DNA lineages (including 269 new sequences from Libya, in the first mtDNA study of the general Libyan population). Our results show a clinal distribution of certain haplogroups, some of them more frequent in Western (H, HV0, L1b, L3b, U6) or Eastern populations (L0a, R0a, N1b, I, J) that might be the result of human migrations from the Middle East, sub-Saharan Africa, and Europe. Despite this clinal pattern, a genetic discontinuity is found in the Libyan/Egyptian border, suggesting a differential gene flow in the Nile River Valley. Finally, frequency of the post-LGM subclades H1 and H3 is predominant in Libya within the H sequences, highlighting the magnitude of the LGM expansion in North Africa. © 2011 Wiley-Liss, Inc.


Weiss E.,University Paris Diderot | Zahar J.-R.,University of Angers | Lesprit P.,Laboratory of Biology | Ruppe E.,University Paris Diderot | And 27 more authors.
Clinical Microbiology and Infection | Year: 2015

Empirical broad spectrum antimicrobial therapy prescribed in life-threatening situations should be de-escalated to mitigate the risk of resistance emergence. Definitions of de-escalation (DE) vary among studies, thereby biasing their results. The aim of this study was to provide a consensus definition of DE and to establish a ranking of β-lactam according to both their spectra and their ecological consequences. Twenty-eight experts from intensive care, infectious disease and clinical microbiology were consulted using the Delphi method (four successive questionnaires) from July to November 2013. More than 70% of similar answers to a question were necessary to reach a consensus. According to our consensus definition, DE purpose was to reduce both the spectrum of antimicrobial therapy and the selective pressure on microbiota. DE included switching from combination to monotherapy. A six-rank consensual classification of β-lactams allowing gradation of DE was established. The group was unable to differentiate ecological consequences of molecules included in group 4, i.e. piperacillin/tazobactam, ticarcillin/clavulanic acid, fourth-generation cephalosporin and antipseudomonal third-generation cephalosporin. Furthermore, no consensus was reached on the delay within which DE should be performed and on whether or not the shortening of antibiotic therapy duration should be included in DE definition. This study provides a consensual ranking of β-lactams according to their global ecological consequences that may be helpful in future studies on DE. However, this work also underlines the difficulties of reaching a consensus on the relative ecological impact of each individual drug and on the timing of DE. © 2015 European Society of Clinical Microbiology and Infectious Diseases.


Vasilev V.,University of Liège | Daly A.F.,University of Liège | Thiry A.,University of Liège | Petrossians P.,University of Liège | And 6 more authors.
Journal of Clinical Endocrinology and Metabolism | Year: 2014

Context: McCune Albright syndrome (MAS) is a clinical association of endocrine and nonendocrine anomalies caused by postzygotic mutation of the GNAS1 gene, leading to somatic activation of the stimulatory α-subunit of G protein (Gsα). Important advances have been made recently in describing pathological characteristics of many MAS-affected tissues, particularly pituitary, testicular, and adrenal disease. Other rarer disease related features are emerging.Objective: The objective of the investigation was to study the pathological and genetic findings of MAS on a tissue-by-tissue basis in classically and nonclassically affected tissues.Design: This was a comprehensive autopsy and genetic analysis.Setting: The study was conducted at a tertiary referral university hospital.Patients: An adult male patient with MAS and severe disease burden including gigantism was the subject of the study.Intervention(s): Interventions included clinical, hormonal, and radiographic studies and gross and microscopic pathology analyses, conventional PCR, and droplet digital PCR analyses of affected and nonaffected tissues.Main Outcome Measure: Pathological findings and the presence of GNAS1 mutations were measured.Results: The patient was diagnosed with MAS syndrome at 6 years of age based on the association of café-au-lait spots and radiological signs of polyostotic fibrous dysplasia. Gigantism developed and hyperprolactinemia, hypogonadotropic hypogonadism, and hyperparathyroidism were diagnosed throughout the adult period. The patient died at the age of 39 years from a pulmonary embolism. A detailed study revealed mosaiscism for the p.R201C GNAS1 mutation distributed across many endocrine and nonendocrine tissues. These genetically implicated tissues included rare or previously undescribed disease associations including primary hyperparathyroidism and hyperplasia of the thymus and endocrine pancreas.Conclusions: This comprehensive pathological study of a single patient highlights the complex clinical profile of MAS and illustrates important advances in understanding the characteristics of somatic GNAS1-related pathology across a wide range of affected organs. Copyright © 2014 by the Endocrine Society.

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