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Geng X.M.,Laboratory of Agricultural Ecology | Geng X.M.,Nanjing Forestry University | Liu J.,Laboratory of Agricultural Ecology | Liu J.,Nanjing Forestry University | And 5 more authors.
Journal of the Faculty of Agriculture, Kyushu University | Year: 2015

Although it is often considered as a senescence phytohormone, abscisic acid (ABA) at low concentration together with sucrose as pulsing solution displayed preservative effect on cut lily flowers. In order to further clarify the preservation mechanism of ABA, the effects of ABA pretreatment on physiological index and ethylene release were investigated. The addition of 2 mg · L-1 ABA to sucrose increased proline content in petals. This result suggested that ABA application resulted in water stress of the cut flowers to some extent. However the pretreatment of the cut lilies with ABA at 2 mg · L-1 delayed the degradation of soluble proteins, and promoted the activity of superoxide dismutase (SOD) enzyme, which decreased the damage to cells induced by reactive oxygen species (ROS). It was also found that the pretreatment with 2mg L-1 ABA inhibited ethylene release.

Geng X.M.,Laboratory of Agricultural Ecology | Geng X.M.,Nanjing Forestry University | Li M.,Laboratory of Agricultural Ecology | Li M.,Nanjing Forestry University | And 5 more authors.
Journal of the Faculty of Agriculture, Kyushu University | Year: 2015

With the delay of harvest stage, the carbohydrate content of flower petals and the diameter of flower buds increased, but the vase life was shortened. Effects of abscisic acid (ABA) and sucrose on preservation of cut lilies were related to the harvest stage. Due to the lower level of soluble sugars and other energy source in early harvested cut lilies, the sucrose pretreatment turned to be particularly effective for extending the vase life and increasing the flower fresh weight and bud diameter. For the cut flowers harvested at middle stage, pretreatment with sucrose or ABA separately enhanced the preservation effect, which was not observed when pretreated with the combination of sucrose and ABA. The preservation effect of the harvested flowers at the late development stage was increased by the ABA pretreatments including ABA alone or ABA and sucrose (ABA+Suc). ABA improved sucrose uptake from the pretreatment solution and increased soluble sugar contents in cut flower petals. Combined with sucrose pretreatment, addition of ABA alleviated leaf chlorosis symptom of cut flowers harvested in early period.

Roan S.-F.,Chinese Culture University | Hsiung T.-C.,Chinese Culture University | Yang K.-Y.,National Taiwan University | Liu W.-T.,National Taiwan University | And 3 more authors.
Journal of the Faculty of Agriculture, Kyushu University | Year: 2013

Papaya seeds are considered as a recalcitrant material for protein preparation in two-dimensional gel electrophoresis (2-DE) because of abundance of interfering compounds. We examined two protein extraction methods, the classical trichloroacetic acid (TCA) / acetone precipitation (TCA-A) and phenol ammonium acetate-methanol precipitation (P-AA-M) methods, to evaluate their compatibility and efficiency of extracting papaya seed proteins in SDS-PAGE and two-dimensional (2D) gel electrophoresis analysis. Compared with TCA-A method, P-AA-M method showed better results, i.e., it increased at least five distinguished bands in SDS-PAGE, reduced problems of streaking and smearing in 2D gel, and enhanced resolution of detectable spots from 124±27 to 717±32. Comparison between traditional Coomassie Brilliant Blue R-250 staining and zinc-reverse staining was also conducted. The result showed that seven times more protein spots could be identified from 2D gel stained with zinc-reverse stain procedure. The resolution of 2D gel analysis for papaya seed proteome was further improved by utilizing pH 4-7 isoelectric focusing gel instead of pH 3-10 one. The protein spots picked from 2D gel through the protocol that we established were compatible with identification assessments of LC-MS/MS. Our data will serve as a platform and reference for future research in proteomics of papaya seeds or other recalcitrant plant materials.

Tateishi N.,Laboratory of Horticultural Science | Ozaki Y.,Laboratory of Horticultural Science | Ozaki Y.,Laboratory of Agricultural Ecology | Okubo H.,Laboratory of Horticultural Science
Journal of the Faculty of Agriculture, Kyushu University | Year: 2010

Partial cDNA sequences of three anthocyanin biosynthetic genes (F3H, flavanone 3-hydroxylase; DFR, dihydroflavonol 4-reductase; ANS, anthocyanidin synthase) were isolated from the petals of Camellia japonica. Their deduced partial amino acid sequences shared high homologies with those of woody plant species (CjF3Ha, 98.0%, CjF3Hb, 91.2% and CjDFR, 99.0% with Camellia sinensis; CjANS, 90.3% with Rhododendron x pulchrum). Some important amino acid residues for enzymatic activities were also conserved in the isolated clones, suggesting that the genes we identified in this study were the homologues of C. japonica. Gene-specific primer pairs were designed based on each partial cDNA sequence. The application of these primer pairs to RT-PCR analyses was tested.

Xuan B.,Laboratory of Horticultural Science | Xuan B.,Thai Nguyen University | Wakana A.,Laboratory of Horticultural Science | Kim J.H.,Laboratory of Horticultural Science | And 3 more authors.
Journal of the Faculty of Agriculture, Kyushu University | Year: 2010

Self-incompatibility S genotypes of 29 Citrus cultivars were estimated by the observation of pollen tube behavior in the lower one-third of styles with the aid of an epifluorescent microscope 7 or 8 days after controlled pollination with restricted number of pollen grains (about 100) on their stigmas. In eight crosses considered to be fully compatible on the basis of segregation distortion of glutamate oxaloacetate transaminase (GOT) isozymes in the progenies of cultivars used for the crosses, the rate of pollen tubes reaching the style base ranged from 7.7% to 24.6% of pollen grains putting on the stigmas, while in twelve crosses considered to be semi-compatible the rate of pollen tubes reaching the style base ranged from 1.6% to 19.3% of pollen grains on the stigmas. The rates were slightly different in different pistillate parents. Twenty cultivars whose genotypes were not determined were pollinated with restricted number of 'Banpeiyu' (S 1S 2) pollen grains and their genotypes were predicted by the pollen tube behaviors. Similarly, 13 controlled crosses were carried out with restricted number of pollen grains and their S genotypes were determined based on the number of pollen tubes reaching the style base. From these results, it became clear that the predicted S genotypes of the cultivars are well correspond with pollen tube behaviors in full or semi compatible crosses and, eventually, it was estimated that 15 cultivars have either S 1 or S 2 allele that 'Banpeiyu' pummelo has.

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