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Manica G.C.M.,Federal University of Parana | Ramos E.A.S.,Federal University of Parana | de Oliveira M.A.S.,Federal University of Parana | Costa F.F.,Northwestern University | And 4 more authors.
Journal of Cancer Science and Therapy | Year: 2013

Invasive ductal carcinoma (IDC) and invasive lobular breast carcinoma (ILC) are the most common malignancies compromising the breast tissue in women worldwide. ILCs tend to form metastasisin remote locations, such as the gastrointestinal tract, peritoneal surface and retroperitoneum, compared with IDCs that move preferentially to the lymph nodes, lung, pleura, liver, brain and bones. The histological characteristics of these two tumors are very similar and there is no useful molecular marker to differentiate IDC from ILC to date. In this study, we cloned and expressed ADAM33 recombinant protein cystein rich domain in order to produce polyclonal antibodies and used it as a new immnunohistochemical molecular biomarker that specifically recognizes the ILC breast cancer subtype. © 2013 Manica GCM, et al.

Bittencourt A.L.,Federal University of Bahia | Oliveira P.D.,Federal University of Bahia | Andrade A.C.,Federal University of Bahia | Santos T.C.,Federal University of Bahia | And 3 more authors.
American Journal of Clinical Pathology | Year: 2013

Objectives: To evaluate the frequency of the different types of cutaneous lymphoma (CL) in 1 university hospital in Brazil and compare this frequency with those observed in other countries. Methods: After review, 72 (84.7%) cases of primary cutaneous T-cell lymphoma (CTCL) and 13 (15.3%) cases of primary cutaneous B-cell lymphoma (CBCL) were included. Results: Of the CTCLs, 40.3% were mycosis fungoides (MF); 26.4% were adult T-cell leukemias/lymphomas (ATLs); 23.6% were peripheral T-cell lymphomas, unspecified; and 8.3% were anaplastic large cell lymphomas. Of the MF cases, 17.2% progressed to transformed MF. Five-year survival for primary human T-cell lymphotropic virus type 1-negative CTCL, ATL, and CBCL was 64.0%, 42.1%, and 62.5%, respectively. MF and ATL were the most frequent primary CTCLs. Conclusions: The frequencies observed here are close to those observed in Peru but different from those of European countries. Unfortunately, the World Health Organization/ European Organization of Research and Treatment of Cancer classification does not include primary cutaneous ATL. © American Society for Clinical Pathology.

Eguia-Aguilar P.,Laboratory of Molecular Biology | Eguia-Aguilar P.,National Autonomous University of Mexico | Perezpena-Diazconti M.,Laboratory of Molecular Biology | Benadon-Darszon E.,Ambulatory Pediatrics | And 6 more authors.
Child's Nervous System | Year: 2014

Purpose: Astrocytomas are the most frequent type of tumor of the central nervous system in children. Hence, it is important to describe markers that may improve our understanding of their behavior. Mature microRNAs (miRNAs) may be such biological markers. They are small molecules of RNA that regulate gene expression post-transcriptionally. Due to their importance in cancer, the objective of the present study was to determine the profile of expression of precursor and mature forms of miR-124-3p, miR-128-1, and miR-221-3p using RT-qPCR in pediatric samples. Methods: A total of 57 astrocytomas embedded in paraffin were selected. As controls, the study included 13 samples of normal brain tissue. Results: Three of eight miRNAs were selected after a preliminary screening. All the miRNAs showed higher levels of expression in normal brain tissue. The expression of miR-124-3p and miR-128-1 decreased in astrocytomas than in normal brain tissue in all grades (p < 0.05 in both cases), and this reduction was most evident in GIV (407- and 1,469-fold, respectively); however, the expression of the precursor forms pre-miR-128-1 and pre-miR-221 was higher in GIV (3.5-fold) than in GI. The levels of miR-128-1 were higher in infratentorial tumors than in supratentorial cases (p = 0.006). Finally, the expression of miR-221-3p was higher in non-recurrent tumors and live patients (p = 0.0185 and p = 0.0004, respectively). Conclusions: The low expression of these miRNAs may constitute a potential marker of astrocytomas that correlates with localization, possibly due to alterations in the maturation processes of these miRNAs that produced low mature forms in patients with recurrent pediatric astrocytomas. © 2014 Springer-Verlag.

Pereira M.C.M.C.,Escola Bahiana de Medicina e Saude Publica Dentistry Course | Pinho C.B.d.,Federal University of Bahia | Medrado A.R.P.,Escola Bahiana de Medicina e Saude Publica Dentistry Course | Andrade Z.d.A.,Laboratory of Experimental Pathology | Reis S.R.d.A.,Escola Bahiana de Medicina e Saude Publica Dentistry Course
Journal of Photochemistry and Photobiology B: Biology | Year: 2010

Laser biomodulation has been getting considerable attention when it comes to its effects on the inflammatory process. Its action upon mast cells have been already studied, but none of the previous papers related the resulting effect to the inflammatory and vascular status of the wounds. Therefore, the acute inflammatory process as well as the mast cells behavior and the vascular response were analyzed under the influence of laser treatment on cutaneous wounds. Surgical procedures were performed on 60 rats divided into sham and laser groups. Low-level laser therapy was performed following surgical procedures (670 nm, 9 mW, 4 J/cm2, 124 s). Histological specimens were analyzed for cell morphology and immunohistochemistry using anti-von Willebrand Factor and anti-Vascular Endothelial Growth Factor antibody. Laser treatment resulted in an increased acute inflammatory response in irradiated tissues; surgical wounds treated with laser therapy had increased polymorphonuclear cells, mast cells and vasodilation and lower numbers of vessels than those in control rats. Laser treatment resulted in higher expression of VEGF in irradiated tissues 6-24 h post-treatment (p = 0.002). It is possible to observe an amplification of acute inflammatory process during the first hours after surgical procedure in rats submitted to laser therapy. © 2010 Elsevier B.V. All rights reserved.

Collatusso C.,Cardiac Surgeon of Santa Casa de Curitiba | Roderjan J.G.,Cardiovascular Grafts Center | Vieira E.D.,Cardiovascular Grafts Center | da Costa F.D.A.,Cardiac Surgery Service of Santa Casa de Curitiba | de Noronha L.,Laboratory of Experimental Pathology
Brazilian Journal of Cardiovascular Surgery | Year: 2012

Objective: The aim of this study was to investigate the SDS-based decellularization process as an anticalcification method in glutaraldehyde-preserved bovine pericardium in subcutaneous rat model. Methods: Pericardium samples with 0.5 cm 2 area and divided into four groups: GDA group: 0.5% glutaraldehydepreserved pericardium (GDA); GDA-GL group: GDA + 0.2% glutamic acid (GL); D-GDA group: decellularized (D) pericardium with 0.1% SDS + GDA, and D-GDA-GL group: decellularized pericardium + GDA + 0.2% glutamic acid. Afterwards these samples were implanted in 18 rats in subcutaneous position up to 90 days. Each animal received samples of the four groups. The explants were performed at 45 and 90 days. The explants were subjected to histology in glass slides stained with hematoxilin-eosin and alizarin red, morphometry evaluation and the calcium content was measured by flame atomic absorption spectrometry. Results: The standard of inflammatory infiltrate was the same in all groups, however more intense in GDA and GDAGL groups in 45 days, increasing at 90 days. The calcium contents for 45 days were: 32.52 ± 3.19 μg/mg in GDA group; 22.12 ± 3.87 μg/mg in GDA-GL group; 1.06 ± 0.38 μg/mg in D-GDA group and 3.99 ± 5.78 μg/mg in D-GDA-GL (P< 0.001). For 90 days were 65.91 ± 24.67 μg/mg in GDA group; 38.37 ± 13.79 μg/mg in GDA-GL group; 1.24 ± 0.99 μg/mg in D-GDA group and 30.54 ± 8.21 μg/mg in D-GDA-GL (P< 0.001). Only D-GDA did not show increase rates of calcium at 45 to 90 days (P=0.314). Conclusion: SDS-based decellularization process reduced the inflammatory intensity and calcification in bovine pericardium in subcutaneous rat model for 90 days.

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