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Manica G.C.M.,Federal University of Paraná | Ramos E.A.S.,Federal University of Paraná | de Oliveira M.A.S.,Federal University of Paraná | Costa F.F.,Northwestern University | And 4 more authors.
Journal of Cancer Science and Therapy | Year: 2013

Invasive ductal carcinoma (IDC) and invasive lobular breast carcinoma (ILC) are the most common malignancies compromising the breast tissue in women worldwide. ILCs tend to form metastasisin remote locations, such as the gastrointestinal tract, peritoneal surface and retroperitoneum, compared with IDCs that move preferentially to the lymph nodes, lung, pleura, liver, brain and bones. The histological characteristics of these two tumors are very similar and there is no useful molecular marker to differentiate IDC from ILC to date. In this study, we cloned and expressed ADAM33 recombinant protein cystein rich domain in order to produce polyclonal antibodies and used it as a new immnunohistochemical molecular biomarker that specifically recognizes the ILC breast cancer subtype. © 2013 Manica GCM, et al.


Bittencourt A.L.,Federal University of Bahia | Oliveira P.D.,Federal University of Bahia | Andrade A.C.,Federal University of Bahia | Santos T.C.,Federal University of Bahia | And 3 more authors.
American Journal of Clinical Pathology | Year: 2013

Objectives: To evaluate the frequency of the different types of cutaneous lymphoma (CL) in 1 university hospital in Brazil and compare this frequency with those observed in other countries. Methods: After review, 72 (84.7%) cases of primary cutaneous T-cell lymphoma (CTCL) and 13 (15.3%) cases of primary cutaneous B-cell lymphoma (CBCL) were included. Results: Of the CTCLs, 40.3% were mycosis fungoides (MF); 26.4% were adult T-cell leukemias/lymphomas (ATLs); 23.6% were peripheral T-cell lymphomas, unspecified; and 8.3% were anaplastic large cell lymphomas. Of the MF cases, 17.2% progressed to transformed MF. Five-year survival for primary human T-cell lymphotropic virus type 1-negative CTCL, ATL, and CBCL was 64.0%, 42.1%, and 62.5%, respectively. MF and ATL were the most frequent primary CTCLs. Conclusions: The frequencies observed here are close to those observed in Peru but different from those of European countries. Unfortunately, the World Health Organization/ European Organization of Research and Treatment of Cancer classification does not include primary cutaneous ATL. © American Society for Clinical Pathology.


Pereira M.C.M.C.,Escola Bahiana de Medicina e Saude Publica Dentistry Course | Pinho C.B.d.,Federal University of Bahia | Medrado A.R.P.,Escola Bahiana de Medicina e Saude Publica Dentistry Course | Andrade Z.d.A.,Laboratory of Experimental Pathology | Reis S.R.d.A.,Escola Bahiana de Medicina e Saude Publica Dentistry Course
Journal of Photochemistry and Photobiology B: Biology | Year: 2010

Laser biomodulation has been getting considerable attention when it comes to its effects on the inflammatory process. Its action upon mast cells have been already studied, but none of the previous papers related the resulting effect to the inflammatory and vascular status of the wounds. Therefore, the acute inflammatory process as well as the mast cells behavior and the vascular response were analyzed under the influence of laser treatment on cutaneous wounds. Surgical procedures were performed on 60 rats divided into sham and laser groups. Low-level laser therapy was performed following surgical procedures (670 nm, 9 mW, 4 J/cm2, 124 s). Histological specimens were analyzed for cell morphology and immunohistochemistry using anti-von Willebrand Factor and anti-Vascular Endothelial Growth Factor antibody. Laser treatment resulted in an increased acute inflammatory response in irradiated tissues; surgical wounds treated with laser therapy had increased polymorphonuclear cells, mast cells and vasodilation and lower numbers of vessels than those in control rats. Laser treatment resulted in higher expression of VEGF in irradiated tissues 6-24 h post-treatment (p = 0.002). It is possible to observe an amplification of acute inflammatory process during the first hours after surgical procedure in rats submitted to laser therapy. © 2010 Elsevier B.V. All rights reserved.


Longuespee R.,University of Liège | Fleron M.,GIGA Research | Pottier C.,Laboratory of Experimental Pathology | Quesada-Calvo F.,University of Liège | And 7 more authors.
OMICS A Journal of Integrative Biology | Year: 2014

The concept of tissues appeared more than 200 years ago, since textures and attendant differences were described within the whole organism components. Instrumental developments in optics and biochemistry subsequently paved the way to transition from classical to molecular histology in order to decipher the molecular contexts associated with physiological or pathological development or function of a tissue. In 1941, Coons and colleagues performed the first systematic integrated examination of classical histology and biochemistry when his team localized pneumonia antigens in infected tissue sections. Most recently, in the early 21st century, mass spectrometry (MS) has progressively become one of the most valuable tools to analyze biomolecular compounds. Currently, sampling methods, biochemical procedures, and MS instrumentations allow scientists to perform "in depth" analysis of the protein content of any type of tissue of interest. This article reviews the salient issues in proteomics analysis of tissues. We first outline technical and analytical considerations for sampling and biochemical processing of tissues and subsequently the instrumental possibilities for proteomics analysis such as shotgun proteomics in an anatomical context. Specific attention concerns formalin fixed and paraffin embedded (FFPE) tissues that are potential "gold mines" for histopathological investigations. In all, the matrix assisted laser desorption/ionization (MALDI) MS imaging, which allows for differential mapping of hundreds of compounds on a tissue section, is currently the most striking evidence of linkage and transition between "classical" and "molecular" histology. Tissue proteomics represents a veritable field of research and investment activity for modern biomarker discovery and development for the next decade. © 2014, Mary Ann Liebert, Inc.


Vilela D.D.C.,Escola Bahiana de Medicina e Saude Publica Dentistry Course | Chamusca F.V.,Escola Bahiana de Medicina e Saude Publica Dentistry Course | Andrade J.C.S.,Laboratory of Experimental Pathology | Vallve M.L.F.,Laboratory of Experimental Pathology | And 4 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2012

This study evaluated the influence of hypothalamic-pituitary-adrenal (HPA) axis in cutaneous wounds subjected to laser biomodulation. A total of 48 rats were divided into two groups: Group I (GI) with 24 adrenalectomized animals and Group II (GII) with 24 non-adrenalectomized animals. Each group was divided into two subgroups: the irradiated subgroup which laser was applied to four points at the edges of the wound (670 nm laser, 9 mW) and control subgroup. Rats in each subgroup were sacrificed at 24 or 72 h. Adrenal glands were only removed from GI rats. Three days after adrenalectomy, a cutaneous wound was made. An immunohistochemical analysis was performed using anti-CD45 and anti-CD8 antibodies. Flow cytometry was used to count T lymphocytes and their subpopulations in blood. Decreases in the number of CD45-positive inflammatory cells and in the total numbers of CD8- and CD45-positive cells were observed in histological sections of adrenalectomized animals subjected to laser biomodulation at 24 h. Similar results were observed for distribution of total lymphocytes in blood (p < 0.05). The action of 670 nm laser does not depend exclusively on HPA axis. It is believed that corticosteroid-promoting enzymes liberated in non-adrenal tissues may influence immune response under the influence of this type of phototherapy. © 2012 Elsevier B.V. All rights reserved.


Collatusso C.,Cardiac Surgeon of Santa Casa de Curitiba | Roderjan J.G.,Cardiovascular Grafts Center | Vieira E.D.,Cardiovascular Grafts Center | da Costa F.D.A.,Hospital Ecoville | de Noronha L.,Laboratory of Experimental Pathology
Brazilian Journal of Cardiovascular Surgery | Year: 2012

Objective: The aim of this study was to investigate the SDS-based decellularization process as an anticalcification method in glutaraldehyde-preserved bovine pericardium in subcutaneous rat model. Methods: Pericardium samples with 0.5 cm 2 area and divided into four groups: GDA group: 0.5% glutaraldehydepreserved pericardium (GDA); GDA-GL group: GDA + 0.2% glutamic acid (GL); D-GDA group: decellularized (D) pericardium with 0.1% SDS + GDA, and D-GDA-GL group: decellularized pericardium + GDA + 0.2% glutamic acid. Afterwards these samples were implanted in 18 rats in subcutaneous position up to 90 days. Each animal received samples of the four groups. The explants were performed at 45 and 90 days. The explants were subjected to histology in glass slides stained with hematoxilin-eosin and alizarin red, morphometry evaluation and the calcium content was measured by flame atomic absorption spectrometry. Results: The standard of inflammatory infiltrate was the same in all groups, however more intense in GDA and GDAGL groups in 45 days, increasing at 90 days. The calcium contents for 45 days were: 32.52 ± 3.19 μg/mg in GDA group; 22.12 ± 3.87 μg/mg in GDA-GL group; 1.06 ± 0.38 μg/mg in D-GDA group and 3.99 ± 5.78 μg/mg in D-GDA-GL (P< 0.001). For 90 days were 65.91 ± 24.67 μg/mg in GDA group; 38.37 ± 13.79 μg/mg in GDA-GL group; 1.24 ± 0.99 μg/mg in D-GDA group and 30.54 ± 8.21 μg/mg in D-GDA-GL (P< 0.001). Only D-GDA did not show increase rates of calcium at 45 to 90 days (P=0.314). Conclusion: SDS-based decellularization process reduced the inflammatory intensity and calcification in bovine pericardium in subcutaneous rat model for 90 days.

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