Laboratory of Epidemiology and Public Health

Fès, Morocco

Laboratory of Epidemiology and Public Health

Fès, Morocco
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Bouletis A.D.,University of Thessaly | Arvanitoyannis I.S.,University of Thessaly | Hadjichristodoulou C.,Laboratory of Epidemiology and Public Health | Neofitou C.,University of Thessaly | And 2 more authors.
International Journal of Food Science and Technology | Year: 2014

The aim of this work was to evaluate the effect of various atmosphere compositions (20% CO2/80% N2 for modified atmosphere packaging (MAP) 1, 50% CO2/50% N2 for MAP 2, 70% CO2/30% N2 for MAP 3 and vacuum packaging) on the microbial (mesophiles, psychrophiles, Pseudomonas spp., Brochothrix thermosphacta and Enterobacteriaceae), physical, chemical [trimethylamine (TMA) and total volatile basic nitrogen (TVBN)] and sensorial characteristics of broadtail squid (Illex coindetii) stored for 10 days at 2 ± 1 °C. All microbial populations were severely restrained by MAP 3 with the exception of Enterobacteriaceae, which seemed to take advantage of the lack of competitive microflora and had enhanced microbial counts on MAP samples (P < 0.05). Colour attributes were better maintained on MAP-stored samples. Drip loss was less on vacuum-packaged squids. MAP 2 was the best atmosphere for the preservation of tissue consistency. TMA and TVBN formation was limited by high CO2 atmospheres, even though both elevated in all studied conditions. Shelf life based on sensory characteristics was determined to be 10, 8, 6, 6 and 4 days for MAP 3, MAP 2, MAP 1, vacuum and control samples, respectively. © 2013 The Authors. International Journal of Food Science and Technology © 2013 Institute of Food Science and Technology.


Bouletis A.D.,University of Thessaly | Arvanitoyannis I.S.,University of Thessaly | Hadjichristodoulou C.,Laboratory of Epidemiology and Public Health | Neofitou C.,University of Thessaly | And 2 more authors.
Journal of the Science of Food and Agriculture | Year: 2016

BACKGROUND: Seafood preservation and its shelf life prolongation are two of the main issues in the seafood industry. As a result, and in view of market globalization, research has been triggered in this direction by applying several techniques such as modified atmosphere packaging (MAP), vacuum packaging (VP) and active packaging (AP). However, seafood such as octopus, cuttlefish and others have not been thoroughly investigated up to now. The aim of this research was to determine the optimal conditions of modified atmosphere under which cuttlefish storage time and consequently shelf life time could be prolonged without endangering consumer safety. RESULTS: It was found that cuttlefish shelf life reached 2, 2, 4, 8 and 8 days for control, VP, MAP 1, MAP 2 and MAP 3 (20% CO2-80% N2, 50% CO2-50% N2 and 70% CO2-30% N2 for MAP 1, 2 and 3, respectively) samples, respectively, judging by their sensorial attributes. Elevated CO2 levels had a strong microbiostatic effect, whereas storage under vacuum did not offer significant advantages. All physicochemical attributes of MAP-treated samples were better preserved compared to control. CONCLUSION: Application of high CO2 atmospheres such as MAP 2 and MAP 3 proved to be an effective strategy toward preserving the characteristics and prolonging the shelf life of fresh cuttlefish and thereby improving its potential in the market. © 2016 Society of Chemical Industry.


PubMed | Laboratory of Epidemiology and Public Health and University of Thessaly
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

Seafood preservation and its shelf life prolongation are two of the main issues in the seafood industry. As a result, and in view of market globalization, research has been triggered in this direction by applying several techniques such as modified atmosphere packaging (MAP), vacuum packaging (VP) and active packaging (AP). However, seafood such as octopus, cuttlefish and others have not been thoroughly investigated up to now. The aim of this research was to determine the optimal conditions of modified atmosphere under which cuttlefish storage time and consequently shelf life time could be prolonged without endangering consumer safety.It was found that cuttlefish shelf life reached 2, 2, 4, 8 and 8 days for control, VP, MAP 1, MAP 2 and MAP 3 (20% CO2 -80% N2 , 50% CO2 -50% N2 and 70% CO2 -30% N2 for MAP 1, 2 and 3, respectively) samples, respectively, judging by their sensorial attributes. Elevated CO2 levels had a strong microbiostatic effect, whereas storage under vacuum did not offer significant advantages. All physicochemical attributes of MAP-treated samples were better preserved compared to control.Application of high CO2 atmospheres such as MAP 2 and MAP 3 proved to be an effective strategy toward preserving the characteristics and prolonging the shelf life of fresh cuttlefish and thereby improving its potential in the market. 2015 Society of Chemical Industry.


Achour S.,University Hospital | Achour S.,Université Ibn Tofail | Rhalem N.,Université Ibn Tofail | Rhalem N.,Moroccan Poison Control and Pharmacovigilance Center | And 7 more authors.
Eastern Mediterranean Health Journal | Year: 2013

In Morocco, acute Atractylis gummifera L. poisoning represents the leading cause of death by plant poisoning especially for children. All cases received in the Moroccan poison control centre from January 1981 to December 2009 (n = 467) were included in a retrospective study of the characteristics and risk factors of A. gummifera L. poisoning The most vulnerable age group was children (63.4% of cases). Most cases were due to accidental exposure (75.5%), but some were from therapeutic use (18.1%) or attempted abortion (7.4%). Patients presented with moderate poison severity signs (grade 2) in 22.3% of cases or severe signs (grade 3) in 21.0%. The mortality rate was 39.2%. The majority of deaths (81.1%) occurred in children aged < 15 years following accidental exposure. Multivariate logistic regression analysis revealed that risk factors for mortality were coma (OR = 20.5); hepatitis (OR = 52.7) and rural residence (OR = 7.26), while gastric decontamination was a protector factor (OR = 0.26).


Abourazzak F.E.,Hassan II University | Kadi N.,Hassan II University | Azzouzi H.,Hassan II University | Lazrak F.,Hassan II University | And 3 more authors.
Open Rheumatology Journal | Year: 2014

Objectives: To compare foot posture in people with and without medial compartment knee osteoarthritis (OA), and to assess association between its abnormalities and medial compartment knee OA. Methods: We compared the foot posture of patients with clinically and radiographically-confirmed medial compartment knee OA and asymptomatic healthy controls using the foot posture index (FPI), navicular height, and the medial arch. Results: We included 100 patients and 80 asymptomatic controls. The mean age of patients was 59 ± 7 (44-76) years and 48 ± 9 (28-60) years in the control (p=0.06). Patients group have more pronated foot for FPI (1.50 ± 2.68 vs 0.72 ± 2.63; p=0.05), more flat foot (42% vs 22%; p=0.03), and less pes cavus than the control group (58% vs 77%; p=0.004). However, there was no significant difference between the groups in the navicular height (3.90 ± 0.85 cm vs 4.00 ± 0.76 cm; p=0.41). In multivariate statistical analysis, after adjusting for age and body mass index, pronated foot in FPI (OR=1.22, 95%IC= [1.06-1.40], p=0.005), and pes cavus (OR=0.32, 95%IC= [0.11-0.93], p=0.03) had a significant correlation with the knee osteoarthritis. Conclusion: Pronated foot posture and flat foot are significantly associated with medial compartment knee osteoarthritis. © Abourazzak et al.; Licensee Bentham Open.


Mansouri S.,University Sidi Mohammed Ben Abdellah | Mansouri S.,Hassan II University | Tahiri L.,University Sidi Mohammed Ben Abdellah | Tahiri L.,Hassan II University | And 14 more authors.
Current Orthopaedic Practice | Year: 2013

Background Pigmented villonodular synovitis (PVNS) is a rare benign condition characterized by villous or nodular hyperplasia of the synovium. Few Moroccan studies on this disease have been reported in the literature. The purpose of this study was to evaluate the clinical, radiographic, and pathologic features and outcomes of PVNS in seven patients. Methods This retrospective study examined data from seven patients with pathologically-confirmed PVNS. Patients were selected from the Rheumatology and Orthopaedic Surgery departments over a period of 2 years (January 2009-December 2010). Collected data included disease localization, therapeuticmodalities, and outcomes. Results Five women and two men were included. The mean age was 30 years (20-50 years). The knee was involved in five patients, the elbow in one patient, and the hip in one patient. Mean duration of symptoms was 36 months (7 months-12 years). Clinical presentation included chronic pain, functional disability, and joint effusion. There was no inflammatory syndrome or coagulation disorder. Joint aspiration was performed in five patients. Radiographs revealed bone abnormalities in two patients. MRI was performed in all patients and showed images characteristic of PVNS. Synovial biopsy confirmed the diagnosis in all patients. All patients underwent surgical synovectomy with good functional results. None had synoviorthesis. Recurrence occurred in one patient 2 months after surgery. The mean follow-up time was 28 ± 6 months. Conclusions The diagnosis of PVNS is purely histological. MRI allows early recognition and improves the early management of this pathology. Synovectomy should be as complete as possible to reduce the risk of recurrence. © 2013 Wolters Kluwer Health.

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