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Falasca L.,Laboratory of Electronic Microscopy
International journal of STD & AIDS | Year: 2012

Giant cell hepatitis (GCH) has been rarely described in adult HIV patients, and its outcome remain unknown. We report two cases of GCH among 81 HIV patients co-infected with the hepatitis C virus (HCV). Both patients had a sustained virological response, suppression of HCV viral load and HIV viral suppression after highly active antiretroviral therapy. Our findings would suggest that the presence of giant cells does not influence the clinical course of hepatitis. Source

Sun B.,Nanjing University | Sun M.-J.,Nanjing University | Sun M.-J.,China Pharmaceutical University | Gu Z.,Nanjing University | And 5 more authors.
Macromolecules | Year: 2010

Bifunctional composite nanoparticles with simultaneous response toward light excitation and external magnetic field are fabricated by electrostatic adsorption of a single layer of conjugated polyelectrolytes on the magnetic nanoparticle surfaces. Cell imaging is realized through incubation of the composite nanoparticles with human hepatoma cell Bel-7402. Both fluorescence microscopy imaging and flow cytometry analysis verify that the bifunctional nanoparticles efficiently penetrate the cell membranes. Transmission electron microscopy reveals that the nanoparticles are confined in the endosome and show clear signs of particle aggregation. The cellular uptake efficiencies of the magnetic-fluorescent nanoparticles can be enhanced greatly by a magnetic field. In vitro cell viability results indicate low cytotoxicity of the nanoparticles even after 72 h incubation. The current method of fluorescence labeling of nanomaterials by electrostatic adsorption is applicable to a variety of charged nanomaterials that exist with great diversity in chemistry and morphology, which is necessary for estimation of cell toxicity and fundamental understanding of phenomena related to the development of nanomaterial based diagnostics and therapeutics. © 2010 American Chemical Society. Source

Liu Y.,Nanjing University | Huang J.,Nanjing University | Sun M.-J.,China Pharmaceutical University | Yu J.-C.,Nanjing University | And 4 more authors.
Nanoscale | Year: 2014

The present study describes a flexible nanoplatform based on electrostatic assembly of conjugated polyelectrolytes (CPEs) and carboxylated multi-walled carbon nanotubes (cMWNTs). It is demonstrated that the obtained nanocomposites inherit intrinsic optical properties of CPEs and characteristic Raman vibration modes of MWNTs, providing a fluorescence-Raman dual-imaging method for intracellular tracking and locating of MWNTs. We suggest that the cellular internalization of the CPE-cMWNT nanocomposites is a surface charge-dependent process. The strengths of this nanoplatform include satisfying biocompatibility, enhanced protein-repellent property, and ease of implementation, making it available for both in vitro and in vivo applications. © 2013 The Royal Society of Chemistry. Source

Fasseas C.,Laboratory of Electronic Microscopy | Akoumianaki-Ioannidou A.,Agricultural University of Athens
Acta Horticulturae | Year: 2010

Laurus nobilis is an evergreen shrub or tree native to the Mediterranean region. Known since ancient times in Greece it has many medicinal and culinary uses, but is mainly used for ornamental purposes in parks and gardens as well as roadside trees. Its leaves are bifacial, hypostomatic and in the temperate zone they are initiated throughout the year whenever the conditions are favourable. During the early stages of development leaves are soft and bright green in colour becoming dark green, hard and leathery when mature. This hardening of the leaves, as is revealed with light and electron microscopy, is due to secondary development of the vascular tissues, thickening and lignification of the walls of the bundle sheath extension cells and the walls of the epidermal cells. In the latter, thickening is more prominent on the adaxial epidermis with all the walls becoming thick and lignified during maturation and to a lesser extent on the abaxial epidermis with the external periclinal walls becoming thicker than the internal. Radial (anticlinal) walls of epidermal cells have field pits apparently not traversed by plasmodesmata and together with the tubular marginal pits of the external (periclinal) wall seem to provide plasticity for water fluctuations. Stomata are sunken with the guard cells embedded in the midregion of the stomatal pore side cell wall of the subsidiary cells thus allowing the opening and closing of the stomata which otherwise would have been difficult due to the hardness of the lignified epidermal cells. These anatomical characteristics allow the plant to withstand long periods of drought and make it an ideal ornamental plant for urban and sub-urban areas in temperate climates. Source

Zavareze E.d.R.,Federal University of Pelotas | Storck C.R.,Federal University of Pelotas | de Castro L.A.S.,Laboratory of Electronic Microscopy | Schirmer M.A.,Federal University of Pelotas | Dias A.R.G.,Federal University of Pelotas
Food Chemistry | Year: 2010

The effect of heat-moisture treatment (HMT) on the properties of rice starches with high-, medium- and low-amylose content was investigated. The starches were adjusted to 15%, 20% and 25% moisture levels, and heated at 110 °C for 1 h. The swelling power, solubility, pasting properties, morphology, enzymatic susceptibility and X-ray crystallinity of the starches were evaluated. HMT reduced the swelling power and solubility of the starches. The strongest effect of HMT occurred on the high-amylose starch; the pasting temperature was increased and the peak viscosity, breakdown, final viscosity and the setback were reduced. HMT increased the starch's susceptibility to α-amylase and promoted a reduction in the starch relative crystallinity. © 2009 Elsevier Ltd. All rights reserved. Source

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