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Maderna E.,Fondazione Istituto Neurologico C. Besta | Corsini E.,Laboratory of Clinical Investigation | Franzini A.,Fondazione Istituto Neurologico C. Besta | Giombini S.,Fondazione Istituto Neurologico C. Besta | And 6 more authors.
Neurological Sciences | Year: 2010

The biological mechanisms associated with the development and rupture of intracranial aneurysms are not fully understood. To clarify the role of VEGF and the related receptors in the pathophysiology of aneurysm, immunostaining for VEGF, VEGFR1 and VEGFR2 was performed on specimens from six unruptured aneurysms and on two specimens of normal arteries wall as a control. The results were correlated with NO concentration of CSF collected during surgery from 8 patients affected by unruptured aneurysms and in 11 control patients. The immunohistochemical data showed a different pattern of VEGF/VEGFR1/VEGFR2 in aneurysms when compared with control. The results of this preliminary study suggest an imbalance of VEGF, VEGFR1 and VEGFR2, and the interaction of VEGF and NO in the pathophysiology of unruptured aneurysms. Our data support the hypothesis of aneurysm formation associated with a loss of expression of VEGFR1, moderate expression of VEGFR2 and high concentration of nitrate. © 2010 Springer-Verlag. Source

Canazza A.,Laboratory of Clinical Investigation | Calatozzolo C.,Laboratory of Clinical Investigation | Calatozzolo C.,Neuropathology Unit | Fumagalli L.,Radiotherapy Unit | And 6 more authors.
Cancer Biology and Therapy | Year: 2011

A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 min. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CKdriven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGFβ at four days (real-time PCR), β1-integrin at 4-5 d (real-time PCR and protein gel blot) and no elevation in phosphorylated AKT at days 4 and 5 (protein gel blot). Our data suggests that glioma cell invasion as well as elevations of TGFβ and β1-integrin are associated with IIR and not CIR. © 2011 Landes Bioscience. Source

Canazza A.,Laboratory of Clinical Investigation | De Grazia U.,Laboratory of Clinical Investigation | Fumagalli L.,Radiotherapy Unit | Brait L.,CyberKnife Unit | And 5 more authors.
Neurological Sciences | Year: 2011

Radiosurgery is used increasingly upon recurrence of high-grade gliomas to deliver a high dose of focused radiation to a defined target. The purpose of our study was to compare intermittent irradiation (IIR) by using a CyberKnife (CK) with continuous irradiation (CIR) by using a conventional linear accelerator (LINAC). A significant decrease in surviving fraction was observed after IIR irradiation compared with after CIR at a dose of 8 Gy. Three hours after irradiation, most of the DNA damage was repaired in U87. Slightly higher basal levels of Ku70/80 mRNA were found in U87 compared with A172, while radiation treatment induced only minor regulation of Ku70/ 80 and Rad51 transcription in either cell lines. IIR treatment using CK significantly decreased the survival in U87 and A172 compared with CIR. Although the two cell lines differed in DNA repair capability, the role of Ku70/80 and Rad51 in the cell line radiosensitivity seemed marginal. © 2011 Springer-Verlag. Source

Shin Y.-K.,U.S. National Institute on Aging | Cong W.-N.,Laboratory of Clinical Investigation | Cai H.,Laboratory of Clinical Investigation | Kim W.,U.S. National Institute on Aging | And 3 more authors.
Journals of Gerontology - Series A Biological Sciences and Medical Sciences | Year: 2012

Normal aging is a complex process that affects every organ system in the body, including the taste system. Thus, we investigated the effects of the normal aging process on taste bud morphology, function, and taste responsivity in male mice at 2, 10, and 18 months of age. The 18-month-old animals demonstrated a significant reduction in taste bud size and number of taste cells per bud compared with the 2-and 10-month-old animals. The 18-month-old animals exhibited a significant reduction of protein gene product 9.5 and sonic hedgehog immunoreactivity (taste cell markers). The number of taste cells expressing the sweet taste receptor subunit, T1R3, and the sweet taste modulating hormone, glucagon-like peptide-1, were reduced in the 18-month-old mice. Concordant with taste cell alterations, the 18-month-old animals demonstrated reduced sweet taste responsivity compared with the younger animals and the other major taste modalities (salty, sour, and bitter) remained intact. © 2011 The Author. Source

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