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De Backer E.,Ghent University | Dubreuil L.,Laboratory of Clinical Bacteriology | Brauman M.,Laboratoires IPRAD | Acar J.,University Pierre and Marie Curie | Vaneechoutte M.,Ghent University
Clinical Microbiology and Infection | Year: 2010

Bacterial vaginosis is a polymicrobial syndrome. The most important marker for bacterial vaginosis is the presence of Gardnerella vaginalis and Atopobium vaginae. In this study, the in vitro susceptibilities to metronidazole and secnidazole of 16 strains of A. vaginae were tested with the agar dilution method. We observed an MIC range for metronidazole of 4-64 mg/L (MIC50, 8 mg/L; MIC90, 32 mg/L) and an MIC range for secnidazole of 4-128 mg/L (MIC50, 16 mg/L; MIC90, 64 mg/L). According to these findings, we can conclude that the activity of secnidazole is similar to that of metronidazole. © 2009 The Authors. Journal Compilation © 2009 European Society of Clinical Microbiology and Infectious Diseases.

Tomanovic S.,University of Belgrade | Chochlakis D.,Laboratory of Clinical Bacteriology | Radulovic Z.,University of Belgrade | Milutinovic M.,University of Belgrade | And 4 more authors.
Experimental and Applied Acarology | Year: 2013

Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s. l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected. © 2012 Springer Science+Business Media B.V.

Sandalakis V.,Regional Laboratory of Public Health | Chochlakis D.,Regional Laboratory of Public Health | Goniotakis I.,Laboratory of Clinical Bacteriology | Tselentis Y.,Regional Laboratory of Public Health | Psaroulaki A.,Laboratory of Clinical Bacteriology
Journal of Water and Health | Year: 2014

In Greece standard tests are performed in the watering and cooling systems of hotels' units either as part of the surveillance scheme or following human infection. The purpose of this study was to establish the minimum inhibitory concentration (MIC) distributions of environmental Legionella isolates for six antimicrobials commonly used for the treatment of Legionella infections, by MIC-test methodology. Water samples were collected from 2004 to 2011 from 124 hotels from the four prefectures of Crete (Greece). Sixty-eight (68) Legionella isolates, comprising L. pneumophila serogroups 1, 2, 3, 5, 6, 8, 12, 13, 15, L. anisa, L. rubrilucens, L. maceachernii, L. quinlivanii, L. oakridgensis, and L. taurinensis, were included in the study. MIC-tests were performed on buffered charcoal yeast extract with α-ketoglutarate, L-cysteine, and ferric pyrophosphate. The MICs were read after 2 days of incubation at 36±1 °C at 2.5%CO2. A large distribution in MICs was recorded for each species and each antibiotic tested. Rifampicin proved to be the most potent antibiotic regardless of the Legionella spp.; tetracycline appeared to have the least activity on our environmental isolates. TheMICtest approach is an easy, although not so cost-effective, way to determine MICs in Legionella spp. These data should be kept inmind especially since these Legionella species may cause human disease. © IWA Publishing 2014.

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